Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 2 June 1989:
Vol. 244. no. 4908, pp. 1057 - 1062
DOI: 10.1126/science.2727693
Prev | Table of Contents | Next

Articles

Science, Vol 244, Issue 4908, 1057-1062
Copyright © 1989 by American Association for the Advancement of Science

articles

Ectopic expression of the serotonin 1c receptor and the triggering of malignant transformation

D Julius, TJ Livelli, TM Jessell, and R Axel

Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY 10032.

Neurotransmitter receptors are usually restricted to neuronal cells, but the signaling pathways activated by these receptors are widely distributed in both neural and non-neural cells. The functional consequences of activating a brain-specific neurotransmitter receptor, the serotonin 5HT1c receptor, in the unnatural environment of a fibroblast were examined. Introduction of functional 5HT1c receptors into NIH 3T3 cells results, at high frequency, in the generation of transformed foci. Moreover, the generation and maintenance of transformed foci requires continued activation of the serotonin receptor. In addition, the injection of cells derived from transformed foci into nude mice results in the generation of tumors. The serotonin 5HT1c receptor therefore functions as a protooncogene when expressed in NIH 3T3 fibroblasts.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Nucleotide P2Y1 receptor regulates EGF receptor mitogenic signaling and expression in epithelial cells.
S. Buvinic, M. Bravo-Zehnder, J. L. Boyer, J. P. Huidobro-Toro, and A. Gonzalez (2007)
J. Cell Sci. 120, 4289-4301
   Abstract »    Full Text »    PDF »
Production and characterization of monoclonal antibodies sensitive to conformation in the 5HT2c serotonin receptor.
F. Mancia, S. Brenner-Morton, R. Siegel, Z. Assur, Y. Sun, I. Schieren, M. Mendelsohn, R. Axel, and W. A. Hendrickson (2007)
PNAS 104, 4303-4308
   Abstract »    Full Text »    PDF »
Platelet-derived serotonin mediates liver regeneration..
M. Lesurtel, R. Graf, B. Aleil, D. J. Walther, Y. Tian, W. Jochum, C. Gachet, M. Bader, and P.-A. Clavien (2006)
Science 312, 104-107
   Abstract »    Full Text »    PDF »
Mammalian G Proteins and Their Cell Type Specific Functions.
N. Wettschureck and S. Offermanns (2005)
Physiol Rev 85, 1159-1204
   Abstract »    Full Text »    PDF »
Mining the Receptorome.
B. N. Armbruster and B. L. Roth (2005)
J. Biol. Chem. 280, 5129-5132
   Full Text »    PDF »
The peripheral cannabinoid receptor Cb2, frequently expressed on AML blasts, either induces a neutrophilic differentiation block or confers abnormal migration properties in a ligand-dependent manner.
M. Alberich Jorda, N. Rayman, M. Tas, S. E. Verbakel, N. Battista, K. van Lom, B. Lowenberg, M. Maccarrone, and R. Delwel (2004)
Blood 104, 526-534
   Abstract »    Full Text »    PDF »
Thrombin Protease-activated Receptor-1 Signals through Gq- and G13-initiated MAPK Cascades Regulating c-Jun Expression to Induce Cell Transformation.
M. J. Marinissen, J.-M. Servitja, S. Offermanns, M. I. Simon, and J. S. Gutkind (2003)
J. Biol. Chem. 278, 46814-46825
   Abstract »    Full Text »    PDF »
Constitutive Inositol Phosphate Formation in Cytomegalovirus-Infected Human Fibroblasts Is due to Expression of the Chemokine Receptor Homologue pUS28.
R. Minisini, C. Tulone, A. Luske, D. Michel, T. Mertens, P. Gierschik, and B. Moepps (2003)
J. Virol. 77, 4489-4501
   Abstract »    Full Text »    PDF »
The peripheral cannabinoid receptor Cb2, a novel oncoprotein, induces a reversible block in neutrophilic differentiation.
M. A. Jorda, B. Lowenberg, and R. Delwel (2003)
Blood 101, 1336-1343
   Abstract »    Full Text »    PDF »
Calpain as an effector of the Gq signaling pathway for inhibition of Wnt/beta -catenin-regulated cell proliferation.
G. Li and R. Iyengar (2002)
PNAS 99, 13254-13259
   Abstract »    Full Text »    PDF »
EGF receptor transactivation is obligatory for protein synthesis stimulation by G protein-coupled receptors.
L. Voisin, S. Foisy, E. Giasson, C. Lambert, P. Moreau, and S. Meloche (2002)
Am J Physiol Cell Physiol 283, C446-C455
   Abstract »    Full Text »    PDF »
Hematopoietic cells expressing the peripheral cannabinoid receptor migrate in response to the endocannabinoid 2-arachidonoylglycerol.
M. Alberich Jorda, S. E. Verbakel, P. J. M. Valk, Y. V. Vankan-Berkhoudt, M. Maccarrone, A. Finazzi-Agro, B. Lowenberg, and R. Delwel (2002)
Blood 99, 2786-2793
   Abstract »    Full Text »    PDF »
Characterization of the murine gammaherpesvirus 68 ORF74 product: a novel oncogenic G protein-coupled receptor.
M. N. Wakeling, D. J. Roy, A. A. Nash, and J. P. Stewart (2001)
J. Gen. Virol. 82, 1187-1197
   Abstract »    Full Text »
Serotonin and the 5-HT2B Receptor in the Development of Enteric Neurons.
E. Fiorica-Howells, L. Maroteaux, and M. D. Gershon (2000)
J. Neurosci. 20, 294-305
   Abstract »    Full Text »    PDF »
ACCELERATED COMMUNICATION: Possible Role of Valvular Serotonin 5-HT2B Receptors in the Cardiopathy Associated with Fenfluramine.
L. W. Fitzgerald, T. C. Burn, B. S. Brown, J. P. Patterson, M. H. Corjay, P. A. Valentine, J.-H. Sun, J. R. Link, I. Abbaszade, J. M. Hollis, et al. (2000)
Mol. Pharmacol. 57, 75-81
   Abstract »    Full Text »
Expression of Serotonin Receptor 2c in Rat Type II Pneumocytes.
D. Wang, M. Post, and E. Cutz (1999)
Am. J. Respir. Cell Mol. Biol. 20, 1175-1180
   Abstract »    Full Text »
Serotonin 5-HT2A receptor induces TGF-beta 1 expression in mesangial cells via ERK: proliferative and fibrotic signals.
J. S. Grewal, Y. V. Mukhin, M. N. Garnovskaya, J. R. Raymond, and E. L. Greene (1999)
Am J Physiol Renal Physiol 276, F922-F930
   Abstract »    Full Text »    PDF »
Inhibition of Constitutive Signaling of Kaposi's Sarcoma-associated Herpesvirus G Protein-Coupled Receptor by Protein Kinases in Mammalian Cells in Culture.
E. Geras-Raaka, L. Arvanitakis, C. Bais, E. Cesarman, E. A. Mesri, and M. C. Gershengorn (1998)
J. Exp. Med. 187, 801-806
   Abstract »    Full Text »    PDF »
Mas Oncogene Signaling and Transformation Require the Small GTP-Binding Protein Rac.
I. E. Zohn, M. Symons, M. Chrzanowska-Wodnicka, J. K. Westwick, and C. J. Der (1998)
Mol. Cell. Biol. 18, 1225-1235
   Abstract »    Full Text »
The Pathways Connecting G Protein-coupled Receptors to the Nucleus through Divergent Mitogen-activated Protein Kinase Cascades.
J. S. Gutkind (1998)
J. Biol. Chem. 273, 1839-1842
   Full Text »    PDF »
The small GTP-binding protein Rho links G protein-coupled receptors and Galpha 12 to the serum response element and to cellular transformation.
C. Fromm, O. A. Coso, S. Montaner, N. Xu, and J. S. Gutkind (1997)
PNAS 94, 10098-10103
   Abstract »    Full Text »    PDF »
Pharmacology of Muscarinic Receptor Subtypes Constitutively Activated by G Proteins.
E. S. Burstein, T. A. Spalding, and M. R. Brann (1997)
Mol. Pharmacol. 51, 312-319
   Abstract »    Full Text »    PDF »
5-HT2B receptor-mediated serotonin morphogenetic functions in mouse cranial neural crest and myocardiac cells.
D. Choi, S. Ward, N Messaddeq, J. Launay, and L Maroteaux (1997)
Development 124, 1745-1755
   Abstract »    PDF »
Agonist Activation of delta -Opioid Receptor but not {micro}-Opioid Receptor Potentiates Fetal Calf Serum or Tyrosine Kinase Receptor-Mediated Cell Proliferation in a CellLine-Specific Manner.
P. Y. Law, T. M. McGinn, K. M. Campbell, L. E. Erickson, and H.H. Loh (1997)
Mol. Pharmacol. 51, 152-160
   Abstract »    Full Text »
Ras Involvement in Signal Transduction by the Serotonin 5-HT2B Receptor.
J.-M. Launay, G. Birraux, D. Bondoux, J. Callebert, D.-S. Choi, S. Loric, and L. Maroteaux (1996)
J. Biol. Chem. 271, 3141-3147
   Abstract »    Full Text »    PDF »
Cell-Based Assays for G-Protein-Coupled/Tyrosine Kinase-Coupled Receptors.
M. R. Brann, T. Messier, C. Dorman, and D. Lannigan (1996)
J Biomol Screen 1, 43-45
   Abstract »    PDF »
Cloning and Analysis of Neurotrophic Factor Receptors Using Function-based Strategies.
D.J. Glass, D.R. Gies, T. Stitt, S. Davis, P. Hantzopoulos, N.Y. Ip, M. Goldfarb, and G.D. Yancopoulos (1992)
Cold Spring Harb Symp Quant Biol 57, 53-62
   Abstract »    PDF »
Growth factors and cancer.
S. Aaronson (1991)
Science 254, 1146-1153
   Abstract »    PDF »
Expression cDNA cloning of the KGF receptor by creation of a transforming autocrine loop.
T Miki, T. Fleming, D. Bottaro, J. Rubin, D Ron, and S. Aaronson (1991)
Science 251, 72-75
   Abstract »    PDF »
Two G protein oncogenes in human endocrine tumors.
J Lyons, C. Landis, G Harsh, L Vallar, K Grunewald, H Feichtinger, Q. Duh, O. Clark, E Kawasaki, H. Bourne, et al. (1990)
Science 249, 655-659
   Abstract »    PDF »
Short-term Electrophysiological Actions of Insulin on Aplysia Neurons: Identification of a Possible Novel Modulatory Second-messenger Mechanism.
J.H. Schwartz, E. Shapiro, S.D. Brown, and A.R. Saltiel (1990)
Cold Spring Harb Symp Quant Biol 55, 95-100
   Abstract »    PDF »
Human Colorectal Cancers Express a Constitutively Active Cholecystokinin-B/Gastrin Receptor That Stimulates Cell Growth.
M. R. Hellmich, X.-L. Rui, H. L. Hellmich, R. Y. D. Fleming, B. M. Evers, and C. M. Townsend Jr. (2000)
J. Biol. Chem. 275, 32122-32128
   Abstract »    Full Text »    PDF »
5-HT2A receptors: location and functional analysis in intestines of wild-type and 5-HT2A knockout mice.
E. Fiorica-Howells, R. Hen, J. Gingrich, Z. Li, and M. D. Gershon (2002)
Am J Physiol Gastrointest Liver Physiol 282, G877-G893
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)