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Science 26 May 1989:
Vol. 244. no. 4907, pp. 971 - 974
DOI: 10.1126/science.2499046
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Articles

Science, Vol 244, Issue 4907, 971-974
Copyright © 1989 by American Association for the Advancement of Science

articles

A G protein gamma subunit shares homology with ras proteins

N Gautam, M Baetscher, R Aebersold, and MI Simon

Division of Biology, California Institute of Technology, Pasadena 91125.

Guanine nucleotide binding proteins (G proteins) that transduce signals from cell surface receptors to effector molecules are made up of three subunits, alpha, beta, and gamma. A complementary DNA clone that encodes a 71-amino acid protein was isolated from bovine brain; this protein contains peptide sequences that were derived from the purified gamma subunit of Gi and Go. The primary sequence of this G protein gamma subunit (G gamma) has 55 percent homology to the gamma subunit of transducin (T gamma) and also has homology to functional domains of mammalian ras proteins. The probe for isolating the clone was generated with the use of the polymerase chain reaction (PCR). The extent of divergence between T gamma and G gamma, the isolation of homologous PCR-generated fragments, and the differences between the predicted amino acid sequence of G gamma and that derived from the gamma subunit of Gi and Go indicate that gamma subunits are encoded by a family of genes.


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