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Science 5 May 1989:
Vol. 244. no. 4904, pp. 582 - 585
DOI: 10.1126/science.2470149
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Articles

Science, Vol 244, Issue 4904, 582-585
Copyright © 1989 by American Association for the Advancement of Science

articles

Commitment of mouse fibroblasts to adipocyte differentiation by DNA transfection

S Chen, LC Teicher, D Kazim, RE Pollack, and LS Wise

Department of Biological Sciences, Columbia University, New York, NY 10027.

Cells of the mouse cell line 3T3-F442A can be induced by various hormones to differentiate into adipocytes, whereas cells of 3T3-C2, a subclone of 3T3, cannot. However, transfection of DNA from uninduced 3T3-F422A cells into 3T3-C2 cells permits recovery of 3T3-C2 transfectants that differentiate into adipocytes in the presence of insulin. DNA isolated from human fat tissue, when transfected into 3T3-C2 mouse cells, also gives rise to mouse transfectants that are induced to differentiate into adipocytes by the addition of insulin. Apparently, transfection of a trans-regulatory gene (or genes) from 3T3-F442A or human fat cells into 3T3-C2 cells is sufficient to commit 3T3-C2 cells to adipocyte differentiation.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Stable stem cell commitment to the adipocyte lineage by inhibition of DNA methylation: Role of the BMP-4 gene.
R. R. Bowers, J. W. Kim, T. C. Otto, and M. D. Lane (2006)
PNAS 103, 13022-13027
   Abstract »    Full Text »    PDF »
pp60Src Mediates Insulin-stimulated Sequestration of the beta 2-Adrenergic Receptor: Insulin Stimulates pp60Src Phosphorylation and Activation.
E. Shumay, X. Song, H.-y. Wang, and C. C. Malbon (2002)
Mol. Biol. Cell 13, 3943-3954
   Abstract »    Full Text »    PDF »
Proadipocyte cell lines: models of cellular proliferation and differentiation.
M. Smyth, R. Sparks, and W Wharton (1993)
J. Cell Sci. 106, 1-9
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