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Science 28 April 1989:
Vol. 244. no. 4903, pp. 466 - 469
DOI: 10.1126/science.2497520
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Articles

Science, Vol 244, Issue 4903, 466-469
Copyright © 1989 by American Association for the Advancement of Science

articles

Functionally distinct NF-kappa B binding sites in the immunoglobulin kappa and IL-2 receptor alpha chain genes

SL Cross, NF Halden, MJ Lenardo, and WJ Leonard

Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892.

The interleukin-2 receptor alpha (IL-2R alpha) chain gene contains a sequence similar to the immunoglobulin (Ig) kappa (kappa) enhancer NF-kappa B binding site. This site, which is bound by the nuclear protein, NF-kappa B, is critical for Ig kappa gene expression. The major T cell nuclear factor that binds to the IL-2R alpha site in vitro appears indistinguishable from NF-kappa B. NF-kappa B binds to IL-2R alpha and kappa sequences with similar affinities; however, only the kappa site potently activates transcription from heterologous promoters. Thus, high-affinity NF-kappa B binding in vitro cannot be equated with transcriptional activation in vivo. Mutation of the NF-kappa B binding site in the context of an IL-2 R alpha promoter construct markedly diminished promoter activity in human T cell lymphotropic virus type I (HTLV-I)-transformed MT-2 cells but not in phorbol myristate acetate-stimulated Jurkat T cells.


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