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Science 21 April 1989: Vol. 244. no. 4902, pp. 359 - 362 DOI: 10.1126/science.2523562
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Articles
Science, Vol 244, Issue 4902, 359-362
Copyright © 1989 by American Association for the Advancement of Science
Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome
QL Choo,
G Kuo,
AJ Weiner,
LR Overby,
DW Bradley,
and
M Houghton
Chiron Corporation, Emeryville, CA 94608.
A random-primed complementary DNA library was constructed from plasma containing the uncharacterized non-A, non-B hepatitis (NANBH) agent and screened with serum from a patient diagnosed with NANBH. A complementary DNA clone was isolated that was shown to encode an antigen associated specifically with NANBH infections. This clone is not derived from host DNA but from an RNA molecule present in NANBH infections that consists of at least 10,000 nucleotides and that is positive-stranded with respect to the encoded NANBH antigen. These data indicate that this clone is derived from the genome of the NANBH agent and are consistent with the agent being similar to the togaviridae or flaviviridae. This molecular approach should be of great value in the isolation and characterization of other unidentified infectious agents.
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- Effect of Hepatitis C Virus (HCV) NS5B-Nucleolin Interaction on HCV Replication with HCV Subgenomic Replicon..
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- From the Cover: Cell culture-grown hepatitis C virus is infectious in vivo and can be recultured in vitro.
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PNAS
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- Analysis of Mutations within the 5' Untranslated Region, Interferon Sensitivity Region, and PePHD Region as a Function of Response to Interferon Therapy in Hepatitis C Virus-Infected Patients in India..
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J. Clin. Microbiol.
44, 709-715
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- SCH 503034, a Mechanism-Based Inhibitor of Hepatitis C Virus NS3 Protease, Suppresses Polyprotein Maturation and Enhances the Antiviral Activity of Alpha Interferon in Replicon Cells.
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Antimicrob. Agents Chemother.
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- Intrahepatic Hepatitis C Virus Replication Correlates with Chronic Hepatitis C Disease Severity In Vivo.
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- Association between Hepatitis C Virus and Very-Low-Density Lipoprotein (VLDL)/LDL Analyzed in Iodixanol Density Gradients.
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- Application of sequence-based methods in human microbial ecology.
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- Hepatitis C: a review for primary care physicians.
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- Management of Hepatitis C Infection in the HIV-Infected Patient.
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Journal of Pharmacy Practice
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- Hepatitis C Virus Induces Toll-Like Receptor 4 Expression, Leading to Enhanced Production of Beta Interferon and Interleukin-6.
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- Selective transmission of hepatitis C virus genotypes and quasispecies in humans and experimentally infected chimpanzees.
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