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Science 21 April 1989:
Vol. 244. no. 4902, pp. 359 - 362
DOI: 10.1126/science.2523562

Articles

Science, Vol 244, Issue 4902, 359-362
Copyright © 1989 by American Association for the Advancement of Science


articles

Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome

QL Choo, G Kuo, AJ Weiner, LR Overby, DW Bradley, and M Houghton

Chiron Corporation, Emeryville, CA 94608.

A random-primed complementary DNA library was constructed from plasma containing the uncharacterized non-A, non-B hepatitis (NANBH) agent and screened with serum from a patient diagnosed with NANBH. A complementary DNA clone was isolated that was shown to encode an antigen associated specifically with NANBH infections. This clone is not derived from host DNA but from an RNA molecule present in NANBH infections that consists of at least 10,000 nucleotides and that is positive-stranded with respect to the encoded NANBH antigen. These data indicate that this clone is derived from the genome of the NANBH agent and are consistent with the agent being similar to the togaviridae or flaviviridae. This molecular approach should be of great value in the isolation and characterization of other unidentified infectious agents.


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B. C. A. Koo, P. McPoland, J. P. Wagoner, O. J. Kane, V. Lohmann, and S. J. Polyak (2006)
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E. Blanchard, S. Belouzard, L. Goueslain, T. Wakita, J. Dubuisson, C. Wychowski, and Y. Rouille (2006)
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Intrahepatic Hepatitis C Virus Replication Correlates with Chronic Hepatitis C Disease Severity In Vivo.
S. Pal, M. C. Shuhart, L. Thomassen, S. S. Emerson, T. Su, N. Feuerborn, J. Kae, and D. R. Gretch (2006)
J. Virol. 80, 2280-2290
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Association between Hepatitis C Virus and Very-Low-Density Lipoprotein (VLDL)/LDL Analyzed in Iodixanol Density Gradients.
S. U. Nielsen, M. F. Bassendine, A. D. Burt, C. Martin, W. Pumeechockchai, and G. L. Toms (2006)
J. Virol. 80, 2418-2428
   Abstract »    Full Text »    PDF »
Application of sequence-based methods in human microbial ecology.
L. Weng, E. M. Rubin, and J. Bristow (2006)
Genome Res. 16, 316-322
   Abstract »    Full Text »    PDF »
Hepatitis C: a review for primary care physicians.
T. Wong and S. S. Lee (2006)
Can. Med. Assoc. J. 174, 649-659
   Abstract »    Full Text »    PDF »
Management of Hepatitis C Infection in the HIV-Infected Patient.
K. McNelis (2006)
Journal of Pharmacy Practice 19, 37-52
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Hepatitis C Virus Induces Toll-Like Receptor 4 Expression, Leading to Enhanced Production of Beta Interferon and Interleukin-6.
K. Machida, K. T. H. Cheng, V. M.-H. Sung, A. M. Levine, S. Foung, and M. M. C. Lai (2006)
J. Virol. 80, 866-874
   Abstract »    Full Text »    PDF »
Selective transmission of hepatitis C virus genotypes and quasispecies in humans and experimentally infected chimpanzees.
O. V. Nainan, L. Lu, F.-X. Gao, E. Meeks, B. H. Robertson, and H. S. Margolis (2006)
J. Gen. Virol. 87, 83-91
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