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Science 21 April 1989:
Vol. 244. no. 4902, pp. 337 - 339
DOI: 10.1126/science.2496466
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Articles

Science, Vol 244, Issue 4902, 337-339
Copyright © 1989 by American Association for the Advancement of Science

articles

Activation of an excluded immunoglobulin allele in a human B lymphoma cell line

N Berinstein, S Levy, and R Levy

Department of Medicine, Stanford University Medical Center, CA 94305.

Mature B cells that express surface immunoglobulin (Ig) are usually committed to their original Ig product. It was shown that such a cell can replace its light chain by rearranging and expressing a new light chain from the other allele. Anti-idiotype antibodies were used to isolate idiotypic variants from a surface IgM+lambda+ human B cell tumor line. The variants expressed a new lambda light chain. Both the original and the new lambda transcripts were present in the variant cells, but only the new one was expressed as a protein on the cell surface. Therefore, although the cell exhibited allelic exclusion and had only one Ig receptor at a time, the commitment to a particular light chain gene was reversible.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Receptor revision of immunoglobulin heavy chain genes in human MALT lymphomas.
D Lenze, A Greiner, C Knorr, I Anagnostopoulos, H Stein, and M Hummel (2003)
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Circulating Human B Cells That Express Surrogate Light Chains Display a Unique Antibody Repertoire.
E. Meffre, M. Chiorazzi, and M. C. Nussenzweig (2001)
J. Immunol. 167, 2151-2156
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Light Chain Shifting: Identification of a Human Plasma Cell Line Actively Undergoing Light Chain Replacement.
H. Tachibana, H. Haruta, and K. Yamada (1999)
Blood 93, 198-207
   Abstract »    Full Text »    PDF »
One-megabase sequence analysis of the human immunoglobulin lambda gene locus..
K Kawasaki, S Minoshima, E Nakato, K Shibuya, A Shintani, J L Schmeits, J Wang, and N Shimizu (1997)
Genome Res. 7, 250-261
   Abstract »    PDF »
Inducing the Loss of Immunoglobulin &lgr;Light Chain Production and the Rearrangement of a Previously Excluded Allele in Human Plasma B Cell Lines with Concanavalin A.
H. Tachibana, Y. Ushio, C. Krungkasem, and S. Shirahata (1996)
J. Biol. Chem. 271, 17404-17410
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