Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 14 April 1989:
Vol. 244. no. 4901, pp. 221 - 224
DOI: 10.1126/science.2539643
Prev | Table of Contents | Next

Articles

Science, Vol 244, Issue 4901, 221-224
Copyright © 1989 by American Association for the Advancement of Science

articles

Expression of a cloned rat brain potassium channel in Xenopus oocytes

MJ Christie, JP Adelman, J Douglass, and RA North

Vollum Institute, Oregon Health Sciences University, Portland 97201.

Potassium channels are ubiquitous membrane proteins with essential roles in nervous tissue, but little is known about the relation between their function and their molecular structure. A complementary DNA library was made from rat hippocampus, and a complementary DNA clone (RBK-1) was isolated. The predicted sequence of the 495-amino acid protein is homologous to potassium channel proteins encoded by the Shaker locus of Drosophila and differs by only three amino acids from the expected product of a mouse clone MBK-1. Messenger RNA transcribed from RBK-1 in vitro directed the expression of potassium channels when it was injected into Xenopus oocytes. The potassium current through the expressed channels resembles both the transient (or A) and the delayed rectifier currents reported in mammalian neurons and is sensitive to both 4-aminopyridine and tetraethylammonium.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Two Opposing Roles of 4-AP-Sensitive K+ Current in Initiation and Invasion of Spikes in Rat Mesencephalic Trigeminal Neurons.
M. Saito, Y. Murai, H. Sato, Y.-C. Bae, T. Akaike, M. Takada, and Y. Kang (2006)
J Neurophysiol 96, 1887-1901
   Abstract »    Full Text »    PDF »
Kv1.3 potassium channels in human alveolar macrophages.
A. B. Mackenzie, H. Chirakkal, and R. A. North (2003)
Am J Physiol Lung Cell Mol Physiol 285, L862-L868
   Abstract »    Full Text »    PDF »
Potassium channels Kv1.1, Kv1.2 and Kv1.6 influence excitability of rat visceral sensory neurons.
P. A Glazebrook, A. N Ramirez, J. H Schild, C.-C. Shieh, T. Doan, B. A Wible, and D. L Kunze (2002)
J. Physiol. 541, 467-482
   Abstract »    Full Text »    PDF »
Functional and molecular expression of a voltage-dependent K+ channel (Kv1.1) in interstitial cells of Cajal.
W. J Hatton, H. S Mason, A. Carl, P. Doherty, M. J Latten, J. L Kenyon, K. M Sanders, and B. Horowitz (2001)
J. Physiol. 533, 315-327
   Abstract »    Full Text »    PDF »
A Slow Transient Potassium Current Expressed in a Subset of Neurosecretory Neurons of the Hypothalamic Paraventricular Nucleus.
J. A. Luther, K. Cs. Halmos, and J. G. Tasker (2000)
J Neurophysiol 84, 1814-1825
   Abstract »    Full Text »    PDF »
Determinants of Apamin and d-Tubocurarine Block in SK Potassium Channels.
T. M. Ishii, J. Maylie, and J. P. Adelman (1997)
J. Biol. Chem. 272, 23195-23200
   Abstract »    Full Text »    PDF »
Identification and Functional Characterization of a K+ Channel alpha -Subunit with Regulatory Properties Specific to Brain.
A. Castellano, M. D. Chiara, B. Mellstrom, A. Molina, F. Monje, J. R. Naranjo, and J. Lopez-Barneo (1997)
J. Neurosci. 17, 4652-4661
   Abstract »    Full Text »    PDF »
Inactivation of a Voltagedependent K+ Channel by beta  Subunit. MODULATION BY A PHOSPHORYLATION-DEPENDENT INTERACTION BETWEEN THE DISTAL C TERMINUS OF alpha  SUBUNIT AND CYTOSKELETON.
J. Jing, T. Peretz, D. Singer-Lahat, D. Chikvashvili, W. B. Thornhill, and I. Lotan (1997)
J. Biol. Chem. 272, 14021-14024
   Abstract »    Full Text »    PDF »
An Antisense Oligonucleotide on the Mouse Shaker-like Potassium Channel Kv1.1 Gene Prevents Antinociception Induced by Morphine and Baclofen.
N. Galeotti, C. Ghelardini, L. Papucci, S. Capaccioli, A. Quattrone, and A. Bartolini (1997)
J. Pharmacol. Exp. Ther. 281, 941-949
   Abstract »    Full Text »
Expression of Kv1.1 Delayed Rectifier Potassium Channels in Lec Mutant Chinese Hamster Ovary Cell Lines Reveals a Role for Sialidation in Channel Function.
W. B. Thornhill, M. B. Wu, X. Jiang, X. Wu, P. T. Morgan, and J. F. Margiotta (1996)
J. Biol. Chem. 271, 19093-19098
   Abstract »    Full Text »    PDF »
Selective Interaction of Voltage-gated K[IMAGE] Channel beta-Subunits with alpha-Subunits.
K. Nakahira, G. Shi, K. J. Rhodes, and J. S. Trimmer (1996)
J. Biol. Chem. 271, 7084-7089
   Abstract »    Full Text »    PDF »
The alpha-Dendrotoxin Footprint on a Mammalian Potassium Channel.
J. Tytgat, T. Debont, E. Carmeliet, and P. Daenens (1995)
J. Biol. Chem. 270, 24776-24781
   Abstract »    Full Text »    PDF »
An amino acid mutation in a potassium channel that prevents inhibition by protein kinase C.
A. Busch, M. Varnum, R. North, and J. Adelman (1992)
Science 255, 1705-1707
   Abstract »    PDF »
A distinct potassium channel polypeptide encoded by the Drosophila eag locus.
J Warmke, R Drysdale, and B Ganetzky (1991)
Science 252, 1560-1562
   Abstract »    PDF »
Biophysical and molecular mechanisms of Shaker potassium channel inactivation.
T Hoshi, W. Zagotta, and R. Aldrich (1990)
Science 250, 533-538
   Abstract »    PDF »
Restoration of inactivation in mutants of Shaker potassium channels by a peptide derived from ShB.
W. Zagotta, T Hoshi, and R. Aldrich (1990)
Science 250, 568-571
   Abstract »    PDF »
K+ current diversity is produced by an extended gene family conserved in Drosophila and mouse.
A Wei, M Covarrubias, A Butler, K Baker, M Pak, and L Salkoff (1990)
Science 248, 599-603
   Abstract »    PDF »
A family of three mouse potassium channel genes with intronless coding regions.
K. Chandy, C. Williams, R. Spencer, B. Aguilar, S Ghanshani, B. Tempel, and G. Gutman (1990)
Science 247, 973-975
   Abstract »    PDF »
Differences in Gating among Amino-terminal Variants of Shaker Potassium Channels.
R.W. Aldrich, T. Hoshi, and W.N. Zagotta (1990)
Cold Spring Harb Symp Quant Biol 55, 19-27
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)