Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 14 April 1989:
Vol. 244. no. 4901, pp. 211 - 214
DOI: 10.1126/science.2704996
Prev | Table of Contents | Next

Articles

Science, Vol 244, Issue 4901, 211-214
Copyright © 1989 by American Association for the Advancement of Science

articles

Regulation of calcium concentration in voltage-clamped smooth muscle cells

PL Becker, JJ Singer, JV Walsh Jr, and FS Fay

Department of Physiology, University of Massachusetts Medical School, Worcester 01655.

The regulation of intracellular calcium concentration in single smooth muscle cells was investigated by simultaneously monitoring electrical events at the surface membrane and calcium concentration in the cytosol. Cytosolic calcium concentration rose rapidly during an action potential or during a voltage-clamp pulse that elicited calcium current; a train of voltage-clamp pulses caused further increases in the calcium concentration up to a limit of approximately 1 microM. The decline of the calcium concentration back to resting levels occurred at rates that varied with the calcium concentration in an apparently saturable manner. Moreover, the rate of decline at any given calcium concentration was enhanced after a higher, more prolonged increase of calcium. The process responsible for this enhancement persisted for many seconds after the calcium concentration returned to resting levels. Thus, the magnitude and duration of a calcium transient appear to regulate the subsequent calcium removal.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A Close Association of RyRs with Highly Dense Clusters of Ca2+-activated Cl- Channels Underlies the Activation of STICs by Ca2+ Sparks in Mouse Airway Smooth Muscle.
R. Bao, L. M. Lifshitz, R. A. Tuft, K. Bellve, K. E. Fogarty, and R. ZhuGe (2008)
J. Gen. Physiol. 132, 145-160
   Abstract »    Full Text »    PDF »
Ca2+ spark sites in smooth muscle cells are numerous and differ in number of ryanodine receptors, large-conductance K+ channels, and coupling ratio between them.
R. ZhuGe, K. E. Fogarty, S. P. Baker, J. G. McCarron, R. A. Tuft, L. M. Lifshitz, and J. V. Walsh Jr. (2004)
Am J Physiol Cell Physiol 287, C1577-C1588
   Abstract »    Full Text »    PDF »
Ca2+ Syntillas, Miniature Ca2+ Release Events in Terminals of Hypothalamic Neurons, Are Increased in Frequency by Depolarization in the Absence of Ca2+ Influx.
V. De Crescenzo, R. ZhuGe, C. Velazquez-Marrero, L. M. Lifshitz, E. Custer, J. Carmichael, F. A. Lai, R. A. Tuft, K. E. Fogarty, J. R. Lemos, et al. (2004)
J. Neurosci. 24, 1226-1235
   Abstract »    Full Text »    PDF »
The Calpain System.
D. E. GOLL, V. F. THOMPSON, H. LI, W. WEI, and J. CONG (2003)
Physiol Rev 83, 731-801
   Abstract »    Full Text »    PDF »
Spontaneous Transient Outward Currents Arise from Microdomains Where BK Channels Are Exposed to a Mean Ca2+ Concentration on the Order of 10 {micro}M during a Ca2+ Spark.
R. ZhuGe, K. E. Fogarty, R. A. Tuft, and J. V. Walsh Jr (2002)
J. Gen. Physiol. 120, 15-28
   Abstract »    Full Text »    PDF »
Cross-bridge regulation by Ca2+-dependent phosphorylation in amphibian smooth muscle.
C. J. Wingard, J. M. Nowocin, and R. A. Murphy (2001)
Am J Physiol Regulatory Integrative Comp Physiol 281, R1769-R1777
   Abstract »    Full Text »    PDF »
Signal Transduction in Smooth Muscle: Invited Review: Mechanisms of calcium handling in smooth muscles.
K. M. Sanders (2001)
J Appl Physiol 91, 1438-1449
   Abstract »    Full Text »    PDF »
Coupling of a P2Z-like purinoceptor to a fatty acid-activated K+ channel in toad gastric smooth muscle cells.
H. Zou, M. Ugur, R. M Drummond, and J. J Singer (2001)
J. Physiol. 534, 59-70
   Abstract »    Full Text »    PDF »
The effect of cyclopiazonic acid on excitation-contraction coupling in guinea-pig ureteric smooth muscle: role of the sarcoplasmic reticulum.
T. V Burdyga and S. Wray (1999)
J. Physiol. 517, 855-865
   Abstract »    Full Text »    PDF »
Mitochondrial regulation of the cytosolic Ca2+ concentration and the InsP3-sensitive Ca2+ store in guinea-pig colonic smooth muscle.
J. G McCarron and T. C Muir (1999)
J. Physiol. 516, 149-161
   Abstract »    Full Text »    PDF »
The Influence of Sarcoplasmic Reticulum Ca2+ Concentration on Ca2+ Sparks and Spontaneous Transient Outward Currents in Single Smooth Muscle Cells.
R. ZhuGe, R. A. Tuft, K. E. Fogarty, K. Bellve, F. S. Fay, and J. V. Walsh Jr. (1999)
J. Gen. Physiol. 113, 215-228
   Abstract »    Full Text »    PDF »
A method for isolating adult and neonatal airway smooth muscle cells and measuring shortening velocity.
S. P. Driska, R. E. Laudadio, M. R. Wolfson, and T. H. Shaffer (1999)
J Appl Physiol 86, 427-435
   Abstract »    Full Text »    PDF »
Properties of voltage-activated [Ca2+]i transients in single smooth muscle cells isolated from pregnant rat uterus.
A V Shmigol, D A Eisner, and S. Wray (1998)
J. Physiol. 511, 803-811
   Abstract »    Full Text »    PDF »
Physiological Features of Visceral Smooth Muscle Cells, With Special Reference to Receptors and Ion Channels.
H. KURIYAMA, K. KITAMURA, T. ITOH, and R. INOUE (1998)
Physiol Rev 78, 811-920
   Abstract »    Full Text »    PDF »
The effects of extracellular and intracellular pH on intracellular Ca2+ regulation in guinea-pig detrusor smooth muscle.
C Wu and C H Fry (1998)
J. Physiol. 508, 131-143
   Abstract »    Full Text »    PDF »
Variability in spontaneous subcellular calcium release in guinea-pig ileum smooth muscle cells.
D V Gordienko, T B Bolton, and M B Cannell (1998)
J. Physiol. 507, 707-720
   Abstract »    Full Text »    PDF »
Calcium-calmodulin-dependent mechanisms accelerate calcium decay in gastric myocytes from Bufo marinus.
J G McGeown, J G McCarron, R M Drummond, and F S Fay (1998)
J. Physiol. 506, 95-107
   Abstract »    Full Text »    PDF »
Changes in intracellular Ca2+ concentration induced by L-type Ca2+ channel current in guinea pig gastric myocytes.
S. J. Kim, S. C. Ahn, J. K. Kim, Y. C. Kim, I. So, and K. W. Kim (1997)
Am J Physiol Cell Physiol 273, C1947-C1956
   Abstract »    Full Text »    PDF »
Regulation of intracellular calcium in human esophageal smooth muscles.
S. M. Sims, Y. Jiao, and H. G. Preiksaitis (1997)
Am J Physiol Cell Physiol 273, C1679-C1689
   Abstract »    Full Text »    PDF »
Predicted changes in concentrations of free and bound ATP and ADP during intracellular Ca2+ signaling.
M. E. Kargacin and G. J. Kargacin (1997)
Am J Physiol Cell Physiol 273, C1416-C1426
   Abstract »    Full Text »    PDF »
Ca2+-induced Ca2+ Release from the Endoplasmic Reticulum Amplifies the Ca2+ Signal Mediated by Activation of Voltage-gated L-type Ca2+ Channels in Pancreatic beta -Cells.
R. Lemmens, O. Larsson, P.-O. Berggren, and MS Islam (2001)
J. Biol. Chem. 276, 9971-9977
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)