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Science 7 April 1989:
Vol. 244. no. 4900, pp. 70 - 72
DOI: 10.1126/science.2468182
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Articles

Science, Vol 244, Issue 4900, 70-72
Copyright © 1989 by American Association for the Advancement of Science

articles

Immune response to cholera toxin epitope inserted in Salmonella flagellin

SM Newton, CO Jacob, and BA Stocker

Department of Microbiology and Immunology, Stanford University School of Medicine, CA 94305.

Bacterial flagella are potent immunogens and aromatic-dependent (aro) Salmonella as live vaccines evoke humoral and cellular immune responses. Such strains expressing epitopes of protective antigens as inserts in flagellin would provide a novel way to vaccinate against diseases caused by unrelated pathogens. A synthetic oligonucleotide specifying an epitope of cholera toxin subunit B was inserted in a Salmonella flagellin gene. The chimeric flagellin functioned normally and the epitope was expressed at the flagellar surface. Parenteral administration to mice of an aro A flagellin-negative strain of S. dublin expressing the chimeric flagellin gene evoked antibody to cholera toxin.


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