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Science 31 March 1989:
Vol. 243. no. 4899, pp. 1681 - 1688
DOI: 10.1126/science.2494700

Articles

Science, Vol 243, Issue 4899, 1681-1688
Copyright © 1989 by American Association for the Advancement of Science


articles

The DNA binding domain of the rat liver nuclear protein C/EBP is bipartite

WH Landschulz, PF Johnson, and SL McKnight

Howard Hughes Medical Institute, Carnegie Institution of Washington, Department of Embryology, Baltimore, MD 21210.

C/EBP is a rat liver nuclear protein capable of sequence-specific interaction with DNA. The DNA sequences to which C/EBP binds in vitro have been implicated in the control of messenger RNA synthesis. It has therefore been predicted that C/EBP will play a role in regulating gene expression in mammalian cells. The region of the C/EBP polypeptide required for direct interaction with DNA has been identified and shown to bear amino acid sequence relatedness with the product of the myc, fos, and jun proto-oncogenes. The arrangement of these related amino acid sequences led to the prediction of a new structural motif, termed the "leucine zipper," that plays a role in facilitating sequence-specific interaction between protein and DNA. Experimental tests now provide support for the leucine zipper hypothesis.


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C. Yu, N. J. Kennedy, C. C.Y. Chang, and J. A. Rothblatt (1996)
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REVIEW {blacksquare} : Regulation of Gene Expression by Neural Signals.
S. E. Hyman (1996)
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Genes & Dev. 8, 350-362
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M Granato, H Schnabel, and R Schnabel (1994)
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M Shirakata, F K Friedman, Q Wei, and B M Paterson (1993)
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HTLV-I Tax protein stimulation of DNA binding of bZIP proteins by enhancing dimerization.
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J W Erickson and T W Cline (1993)
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C R Vinson, T Hai, and S M Boyd (1993)
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Altered specificity of DNA-binding proteins with transition metal dimerization domains.
B Cuenoud and A Schepartz (1993)
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Tissue-specific RNA splicing generates an ankyrin-like domain that affects the dimerization and DNA-binding properties of a bHLH protein..
E S Klein, D M Simmons, L W Swanson, and M G Rosenfeld (1993)
Genes & Dev. 7, 55-71
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Cell phenotype, binding affinity and promoter structure modulate transactivation by HNF1 and LAP.
P. O. Angrand, J. P. Rousset, and M. C. Weiss (1992)
J. Cell Sci. 103, 1083-1092
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Drosophila C/EBP: a tissue-specific DNA-binding protein required for embryonic development..
P Rorth and D J Montell (1992)
Genes & Dev. 6, 2299-2311
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J A Nye, J M Petersen, C V Gunther, M D Jonsen, and B J Graves (1992)
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CHOP, a novel developmentally regulated nuclear protein that dimerizes with transcription factors C/EBP and LAP and functions as a dominant-negative inhibitor of gene transcription..
D Ron and J F Habener (1992)
Genes & Dev. 6, 439-453
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A conserved regulatory unit implicated in tissue-specific gene expression in Drosophila and man..
D Falb and T Maniatis (1992)
Genes & Dev. 6, 454-465
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A Drosophila CREB/ATF transcriptional activator binds to both fat body- and liver-specific regulatory elements..
T Abel, R Bhatt, and T Maniatis (1992)
Genes & Dev. 6, 466-480
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Predicted structural similarities of the DNA binding domains of c-Myc and endonuclease Eco RI.
T. Halazonetis and A. Kandil (1992)
Science 255, 464-466
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DNA-binding properties of the HMG domain of the lymphoid-specific transcriptional regulator LEF-1..
K Giese, A Amsterdam, and R Grosschedl (1991)
Genes & Dev. 5, 2567-2578
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DNA bending by Fos and Jun: the flexible hinge model.
T. Kerppola and T Curran (1991)
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A dominant negative form of transcription activator mTFE3 created by differential splicing.
C Roman, L Cohn, and K Calame (1991)
Science 254, 94-97
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TEF, a transcription factor expressed specifically in the anterior pituitary during embryogenesis, defines a new class of leucine zipper proteins..
D W Drolet, K M Scully, D M Simmons, M Wegner, K T Chu, L W Swanson, and M G Rosenfeld (1991)
Genes & Dev. 5, 1739-1753
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Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells..
Z Cao, R M Umek, and S L McKnight (1991)
Genes & Dev. 5, 1538-1552
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A family of C/EBP-related proteins capable of forming covalently linked leucine zipper dimers in vitro..
S C Williams, C A Cantwell, and P F Johnson (1991)
Genes & Dev. 5, 1553-1567
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Transcriptional regulation of a pair-rule stripe in Drosophila..
S Small, R Kraut, T Hoey, R Warrior, and M Levine (1991)
Genes & Dev. 5, 827-839
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Analysis of the DNA-binding and activation properties of the human transcription factor AP-2..
T Williams and R Tjian (1991)
Genes & Dev. 5, 670-682
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Characterization of a dimerization motif in AP-2 and its function in heterologous DNA-binding proteins.
T Williams and R Tjian (1991)
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An arginine to lysine substitution in the bZIP domain of an opaque-2 mutant in maize abolishes specific DNA binding..
M J Aukerman, R J Schmidt, B Burr, and F A Burr (1991)
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CCAAT-enhancer binding protein: a component of a differentiation switch.
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Sequence requirements for coiled-coils: analysis with lambda repressor-GCN4 leucine zipper fusions.
J. Hu, E. O'Shea, P. Kim, and R. Sauer (1990)
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   PDF »
LAP, a novel member of the C/EBP gene family, encodes a liver-enriched transcriptional activator protein..
P Descombes, M Chojkier, S Lichtsteiner, E Falvey, and U Schibler (1990)
Genes & Dev. 4, 1541-1551
   Abstract »    PDF »
Sequence-specific DNA binding by a short peptide dimer.
R. Talanian, C. McKnight, and P. Kim (1990)
Science 249, 769-771
   Abstract »    PDF »
Evidence of changes in protease sensitivity and subunit exchange rate on DNA binding by C/EBP.
J. Shuman, C. Vinson, and S. McKnight (1990)
Science 249, 771-774
   Abstract »    PDF »
Ig/EBP-1: a ubiquitously expressed immunoglobulin enhancer binding protein that is similar to C/EBP and heterodimerizes with C/EBP..
C Roman, J S Platero, J Shuman, and K Calame (1990)
Genes & Dev. 4, 1404-1415
   Abstract »    PDF »
Identification of two polypeptide segments of CCAAT/enhancer-binding protein required for transcriptional activation of the serum albumin gene..
A D Friedman and S L McKnight (1990)
Genes & Dev. 4, 1416-1426
   Abstract »    PDF »
Structural motif of the GCN4 DNA binding domain characterized by affinity cleaving.
M. Oakley and P. Dervan (1990)
Science 248, 847-850
   Abstract »    PDF »
Sequence-specific DNA binding of the proto-oncoprotein ets-1 defines a transcriptional activator sequence within the long terminal repeat of the Moloney murine sarcoma virus..
C V Gunther, J A Nye, R S Bryner, and B J Graves (1990)
Genes & Dev. 4, 667-679
   Abstract »    PDF »



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Science. ISSN 0036-8075 (print), 1095-9203 (online)