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Science 17 March 1989:
Vol. 243. no. 4897, pp. 1467 - 1469
DOI: 10.1126/science.2648570

Articles

Science, Vol 243, Issue 4897, 1467-1469
Copyright © 1989 by American Association for the Advancement of Science


articles

Endothelial cell gene expression of a neutrophil chemotactic factor by TNF-alpha, LPS, and IL-1 beta

RM Strieter, SL Kunkel, HJ Showell, DG Remick, SH Phan, PA Ward, and RM Marks

Department of Internal Medicine, University of Michigan Medical School, Ann Arbor 48109.

Human endothelial cells produced a neutrophil chemotactic factor (NCF) upon stimulation with tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), or lipopolysaccharide (LPS). The expression of endothelial cell-derived NCF messenger RNA and biological activity was both time- and concentration-dependent. Maximal NCF mRNA expression occurred at 10 and at 2 nanograms per milliliter for TNF and IL-1 beta, respectively; mRNA expression was first observed 1 hour after stimulation and was maintained for at least 24 hours. In situ hybridization analysis showed that NCF mRNA peaked in treated cells by 24 hours, whereas unstimulated cells were negative. These studies demonstrated that endothelial cells may participate in neutrophil-mediated inflammation by synthesizing a chemotactic factor in response to specific monokines and LPS.


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INFLUENCE OF DURAFLO II HEPARIN-TREATED EXTRACORPOREAL CIRCUITS ON THE SYSTEMIC INFLAMMATORY RESPONSE IN PATIENTS HAVING CORONARY BYPASS.
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The Functional Role of the ELR Motif in CXC Chemokine-mediated Angiogenesis.
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Circulation 92, 315-321
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Ischemia-Induced Interleukin-8 Release After Human Heart Transplantation : A Potential Role for Endothelial Cells.
M. C. Oz, H. Liao, Y. Naka, A. Seldomridge, D. N. Becker, R. E. Michler, C. R. Smith, E. A. Rose, D. M. Stern, and D. J. Pinsky (1995)
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Early Intrathecal Production of Interleukin-6 Predicts the Size of Brain Lesion in Stroke.
E. Tarkowski, L. Rosengren, C. Blomstrand, C. Wikkelso, C. Jensen, S. Ekholm, and A. Tarkowski (1995)
Stroke 26, 1393-1398
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Identification and Characterization of a Novel Cytokine-inducible Nuclear Protein from Human Endothelial Cells.
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Role of Neutrophils in Myocardial Ischemia and Reperfusion.
P. R. Hansen (1995)
Circulation 91, 1872-1885
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Venous Thrombosis–Associated Inflammation and Attenuation With Neutralizing Antibodies to Cytokines and Adhesion Molecules.
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Arterioscler Thromb Vasc Biol 15, 258-268
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Interleukin-8 Expression by Head and Neck Squamous Cell Carcinoma.
R. F. Cohen, J. Contrino, J. D. Spiro, E. A. Mann, L. L. Chen, and D. L. Kreutzer (1995)
Arch Otolaryngol Head Neck Surg 121, 202-209
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Respiratory Disease in the Immunosuppressed Patient.
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Cytokine and Growth Factor Release by Alveolar Macrophages: Potential Biomarkers of Pulmonary Toxicity*1.
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Background Review and Current Concepts of Reperfusion Injury.
J. M. Hallenbeck and A. J. Dutka (1990)
Arch Neurol 47, 1245-1254
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Endothelial interleukin-8: a novel inhibitor of leukocyte-endothelial interactions.
M. Gimbrone Jr, M. Obin, A. Brock, E. Luis, P. Hass, C. Hebert, Y. Yip, D. Leung, D. Lowe, W. Kohr, et al. (1989)
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Multiple System Organ Failure in the Adult Respiratory Distress Syndrome.
S. J. Heyman and J. E. Rinaldo (1989)
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