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Science 10 March 1989:
Vol. 243. no. 4896, pp. 1367 - 1368
DOI: 10.1126/science.2922614
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Articles

Science, Vol 243, Issue 4896, 1367-1368
Copyright © 1989 by American Association for the Advancement of Science

articles

Free calcium at rest during "catch" in single smooth muscle cells

N Ishii, AW Simpson, and CC Ashley

Zoological Institute, Faculty of Science, University of Tokyo, Japan.

Tension and intracellular free calcium concentration [( Ca2+]i) were measured simultaneously in single smooth muscle cells isolated from the anterior byssus retractor muscle (ABRM) of Mytilus edulis that were loaded with the fluorescent Ca2+ indicator fura-2. Electrical stimulation evoked a transient elevation of [Ca2+]i associated with a "catch" contraction. During the catch state, however, [Ca2+]i was effectively at its resting level and was unaffected by 5-hydroxytryptamine, which induced a rapid relaxation from catch. The results indicate that a maintained high [Ca2+]i is not required for the maintenance of catch tension in intact ABRM and that there was no significant change in [Ca2+]i upon abolition of catch.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Myosin Loop 2 Is Involved in the Formation of a Trimeric Complex of Twitchin, Actin, and Myosin.
D. Funabara, R. Osawa, M. Ueda, S. Kanoh, D. J. Hartshorne, and S. Watabe (2009)
J. Biol. Chem. 284, 18015-18020
   Abstract »    Full Text »    PDF »
Unphosphorylated twitchin forms a complex with actin and myosin that may contribute to tension maintenance in catch.
D. Funabara, C. Hamamoto, K. Yamamoto, A. Inoue, M. Ueda, R. Osawa, S. Kanoh, D. J. Hartshorne, S. Suzuki, and S. Watabe (2007)
J. Exp. Biol. 210, 4399-4410
   Abstract »    Full Text »    PDF »
The catch state of mollusc catch muscle is established during activation: experiments on skinned fibre preparations of the anterior byssus retractor muscle of Mytilus edulis L. using the myosin inhibitors orthovanadate and blebbistatin.
O. Andruchov, O. Andruchova, and S. Galler (2006)
J. Exp. Biol. 209, 4319-4328
   Abstract »    Full Text »    PDF »
Effect of pH on the rate of myosin head detachment in molluscan catch muscle: are myosin heads involved in the catch state?.
M. C. Hopflinger, O. Andruchova, O. Andruchov, H. Grassberger, and S. Galler (2006)
J. Exp. Biol. 209, 668-676
   Abstract »    Full Text »    PDF »
Marked load-bearing ability of Mytilus smooth muscle in both active and catch states as revealed by quick increases in load.
M. Mukou, H. Kishi, I. Shirakawa, T. Kobayashi, K. Tominaga, H. Imanishi, and H. Sugi (2004)
J. Exp. Biol. 207, 1675-1681
   Abstract »    Full Text »    PDF »
Twitchin from Molluscan Catch Muscle: PRIMARY STRUCTURE AND RELATIONSHIP BETWEEN SITE-SPECIFIC PHOSPHORYLATION AND MECHANICAL FUNCTION.
D. Funabara, S. Watabe, S. U. Mooers, S. Narayan, C. Dudas, D. J. Hartshorne, M. J. Siegman, and T. M. Butler (2003)
J. Biol. Chem. 278, 29308-29316
   Abstract »    Full Text »    PDF »
Phosphorylation of a twitchin-related protein controls catch and calcium sensitivity of force production in invertebrate smooth muscle.
M. J. Siegman, D. Funabara, S. Kinoshita, S. Watabe, D. J. Hartshorne, and T. M. Butler (1998)
PNAS 95, 5383-5388
   Abstract »    Full Text »    PDF »
An in vitro assay reveals essential protein components for the "catch" state of invertebrate smooth muscle.
A. Yamada, M. Yoshio, H. Kojima, and K. Oiwa (2001)
PNAS 98, 6635-6640
   Abstract »    Full Text »    PDF »


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