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Science 3 March 1989:
Vol. 243. no. 4895, pp. 1196 - 1198
DOI: 10.1126/science.2466338
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Articles

Science, Vol 243, Issue 4895, 1196-1198
Copyright © 1989 by American Association for the Advancement of Science

articles

Signal peptide for protein secretion directing glycophospholipid membrane anchor attachment

IW Caras and GN Weddell

Genentech, South San Francisco, CA 94080.

Decay accelerating factor (DAF) is anchored to the plasma membrane by a glycophospholipid (GPI) membrane anchor covalently attached to the COOH-terminus of the protein. A hydrophobic domain located at the COOH-terminus is required for anchor attachment; DAF molecules lacking this domain are secreted. Replacement of the COOH-terminal hydrophobic domain with a signal peptide that normally functions in membrane translocation, or with a random hydrophobic sequence, results in efficient and correct processing, producing GPI-anchored DAF on the cell surface. The structural requirements for GPI anchor attachment and for membrane translocation are therefore similar, presumably depending on overall hydrophobicity rather than specific sequences.


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