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Science 17 February 1989:
Vol. 243. no. 4893, pp. 937 - 940
DOI: 10.1126/science.2521957
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Articles

Science, Vol 243, Issue 4893, 937-940
Copyright © 1989 by American Association for the Advancement of Science

articles

Point mutational inactivation of the retinoblastoma antioncogene

JM Horowitz, DW Yandell, SH Park, S Canning, P Whyte, K Buchkovich, E Harlow, RA Weinberg, and TP Dryja

Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge 02142.

The retinoblastoma (Rb) antioncogene encodes a nuclear phosphoprotein, p105-Rb, that forms protein complexes with the adenovirus E1A and SV40 large T oncoproteins. A novel, aberrant Rb protein detected in J82 bladder carcinoma cells was not able to form a complex with E1A and was less stable than p105-Rb. By means of a rapid method for the detection of mutations in Rb mRNA, this defective Rb protein was observed to result from a single point mutation within a splice acceptor sequence in J82 genomic DNA. This mutation eliminates a single exon and 35 amino acids from its encoded protein product.


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