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Science 10 February 1989:
Vol. 243. no. 4892, pp. 796 - 800
DOI: 10.1126/science.2644694
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Articles

Science, Vol 243, Issue 4892, 796-800
Copyright © 1989 by American Association for the Advancement of Science

articles

Disruption of the yeast N-myristoyl transferase gene causes recessive lethality

RJ Duronio, DA Towler, RO Heuckeroth, and JI Gordon

Department of Biological Chemistry, Washington University School of Medicine, St. Louis, MO 63110.

The structural gene for N-myristoyl transferase (NMT1) has been cloned from the budding yeast Saccharomyces cerevisiae. The gene encodes a polypeptide of 455 amino acids (Mr = 52,837) that has no identifiable significant primary sequence homology with any protein in currently available databases. Overexpression of NMT activity was achieved by means of the yeast episomal plasmid YEp24 without obvious effects on growth kinetics, cell morphology, or acylprotein metabolic labeling patterns. Insertional mutagenesis of the NMT1 locus on yeast chromosome XII caused recessive lethality, indicating that this protein acyltransferase activity is necessary for vegetative cell growth.


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