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Science 3 February 1989:
Vol. 243. no. 4891, pp. 666 - 669
DOI: 10.1126/science.2464853
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Articles

Science, Vol 243, Issue 4891, 666-669
Copyright © 1989 by American Association for the Advancement of Science

articles

Specific block of calcium channel expression by a fragment of dihydropyridine receptor cDNA

I Lotan, P Goelet, A Gigi, and N Dascal

Division of Physiology and Pharmacology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, Israel.

Although the structure of rabbit skeletal muscle dihydropyridine (DHP) receptor, deduced from cDNA sequence, indicates that this protein is the channel-forming subunit of voltage-dependent calcium channel (VDCC), no functional proof for this prediction has been presented. Two DNA oligonucleotides complementary to DHP-receptor RNA sequences coding for putative membrane-spanning regions of the DHP receptor specifically suppress the expression of the DHP-sensitive VDCC from rabbit and rat heart in Xenopus oocytes. However, these oligonucleotides do not suppress the expression of the DHP-insensitive VDCC and of voltage-dependent sodium and potassium channels. Thus, the gene for DHP receptor of rabbit skeletal muscle is closely related, or identical to, a gene expressed in heart that encodes a component of the DHP-sensitive VDCC. The DHP-sensitive and DHP-insensitive VDCCs are distinct molecular entities.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The roles of the subunits in the function of the calcium channel.
D Singer, M Biel, I Lotan, V Flockerzi, F Hofmann, and N Dascal (1991)
Science 253, 1553-1557
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