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Science 3 February 1989:
Vol. 243. no. 4891, pp. 622 - 628
DOI: 10.1126/science.2464850
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Articles

Science, Vol 243, Issue 4891, 622-628
Copyright © 1989 by American Association for the Advancement of Science

articles

Protein design, a minimalist approach

WF DeGrado, ZR Wasserman, and JD Lear

E. I. du Pont de Nemours & Company, Central Research and Development Department, Wilmington, DE 19898.

The question of how the amino acid sequence of a protein specifies its three-dimensional structure remains to be answered. Proteins are so large and complex that it is difficult to discern the features in their sequences that contribute to their structural stability and function. One approach to this problem is de novo design of model proteins, much simpler than their natural counterparts, yet containing sufficient information in their sequences to specify a given function (for example, folding in aqueous solution, folding in membranes, or formation of ion channels). Designed proteins provide simple model systems for understanding protein structure and function.


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