Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 27 January 1989:
Vol. 243. no. 4890, pp. 544 - 546
DOI: 10.1126/science.2536195
Prev | Table of Contents | Next

Articles

Science, Vol 243, Issue 4890, 544-546
Copyright © 1989 by American Association for the Advancement of Science

articles

Repression of the IgH enhancer in teratocarcinoma cells associated with a novel octamer factor

MJ Lenardo, L Staudt, P Robbins, A Kuang, RC Mulligan, and D Baltimore

Whitehead Institute for Biomedical Research, Cambridge, MA 02142.

Embryonal carcinoma (EC) cell lines are models for early cells in mouse embryogenesis. A 300-base pair fragment of the heavy chain enhancer was inactive in F9 EC cells, unlike in other nonlymphoid cells where it has significant activity. Alterations of the octamer motif increased enhancer activity. Nuclear extracts from F9 cells contained an octamer binding protein (NF-A3) that was unique to EC cells; the amount of NF-A3 decreased upon differentiation. It is proposed that NF-A3 represses specific regulatory sequences that contain the octamer motif. Thus, the same DNA sequence mediates either negative or positive transcriptional effects, depending on the cell type.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Expression of the Embryonic Transcription Factor Oct4 in Canine Neoplasms: A Potential Marker for Stem Cell Subpopulations in Neoplasia.
J. D. Webster, V. Yuzbasiyan-Gurkan, J. E. Trosko, C.-C Chang, and M. Kiupel (2007)
Vet. Pathol. 44, 893-900
   Abstract »    Full Text »    PDF »
Oct-4 mRNA and protein expression during human preimplantation development.
G. Cauffman, H. Van de Velde, I. Liebaers, and A. Van Steirteghem (2005)
Mol. Hum. Reprod. 11, 173-181
   Abstract »    Full Text »    PDF »
Genetically targeted radiotherapy for multiple myeloma.
D. Dingli, R. M. Diaz, E. R. Bergert, M. K. O'Connor, J. C. Morris, and S. J. Russell (2003)
Blood 102, 489-496
   Abstract »    Full Text »    PDF »
Phenotypic Complementation Establishes Requirements for Specific POU Domain and Generic Transactivation Function of Oct-3/4 in Embryonic Stem Cells.
H. Niwa, S. Masui, I. Chambers, A. G. Smith, and J.-i. Miyazaki (2002)
Mol. Cell. Biol. 22, 1526-1536
   Abstract »    Full Text »    PDF »
A Single 3'{alpha} hs1,2 Enhancer in the Rabbit IgH Locus.
V. V. Volgina, M. Kingzette, S.-K. Zhai, and K. L. Knight (2000)
J. Immunol. 165, 6400-6405
   Abstract »    Full Text »    PDF »
Synergy of SF1 and RAR in Activation of Oct-3/4 Promoter.
E. Barnea and Y. Bergman (2000)
J. Biol. Chem. 275, 6608-6619
   Abstract »    Full Text »    PDF »
Transcriptional Regulation of the Cellular Retinoic Acid Binding Protein I Gene in F9 Teratocarcinoma Cells.
A. L. Means, J. R. Thompson, and L. J. Gudas (2000)
Cell Growth Differ. 11, 71-82
   Abstract »    Full Text »
Molecular Cloning, Genetic Mapping, and Developmental Expression of Bovine POU5F1.
M.J.T. van Eijk, M.A. van Rooijen, S. Modina, L. Scesi, G. Folkers, H.T.A. van Tol, M.M. Bevers, S.R. Fisher, H.A. Lewin, D. Rakacolli, et al. (1999)
Biol Reprod 60, 1093-1103
   Abstract »    Full Text »
Rex-1, a Gene Encoding a Transcription Factor Expressed in the Early Embryo, Is Regulated via Oct-3/4 and Oct-6 Binding to an Octamer Site and a Novel Protein, Rox-1, Binding to an Adjacent Site.
E. Ben-Shushan, J. R. Thompson, L. J. Gudas, and Y. Bergman (1998)
Mol. Cell. Biol. 18, 1866-1878
   Abstract »    Full Text »
Silencing of the Gene for the beta Subunit of Human Chorionic Gonadotropin by the Embryonic Transcription Factor Oct-3/4.
L. Liu and R. M. Roberts (1996)
J. Biol. Chem. 271, 16683-16689
   Abstract »    Full Text »    PDF »
The POU domain: versatility in transcriptional regulation by a flexible two-in-one DNA-binding domain..
W Herr and M A Cleary (1995)
Genes & Dev. 9, 1679-1693
   PDF »
Oct-3 and the beginning of mammalian development.
M. Rosner, M. Vigano, P. Rigby, H Arnheiter, and L. Staudt (1991)
Science 253, 144-145
   PDF »
Interactions of the Oct-1 POU subdomains with specific DNA sequences and with the HSV alpha-trans-activator protein..
T M Kristie and P A Sharp (1990)
Genes & Dev. 4, 2383-2396
   Abstract »    PDF »
Regulation of gene expression with double-stranded phosphorothioate oligonucleotides.
A Bielinska, R. Shivdasani, L. Zhang, and G. Nabel (1990)
Science 250, 997-1000
   Abstract »    PDF »
The oct-1 homeo domain contacts only part of the octamer sequence and full oct-1 DNA-binding activity requires the POU-specific domain..
C P Verrijzer, A J Kal, and P C van der Vliet (1990)
Genes & Dev. 4, 1964-1974
   Abstract »    PDF »
Expression and activity of the POU transcription factor SCIP.
E. Monuki, R Kuhn, G Weinmaster, B. Trapp, and G Lemke (1990)
Science 249, 1300-1303
   Abstract »    PDF »
Activation of an intron enhancer within the keratin 18 gene by expression of c-fos and c-jun in undifferentiated F9 embryonal carcinoma cells..
R G Oshima, L Abrams, and D Kulesh (1990)
Genes & Dev. 4, 835-848
   Abstract »    PDF »
The E2 trans-activator can act as a repressor by interfering with a cellular transcription factor..
A Stenlund and M R Botchan (1990)
Genes & Dev. 4, 123-136
   Abstract »    PDF »
Retrovirus activation in embryonal carcinoma cells by cellular promoters..
I Peckham, S Sobel, J Comer, R Jaenisch, and E Barklis (1989)
Genes & Dev. 3, 2062-2071
   Abstract »    PDF »
Cooperative Activation by GATA-4 and YY1 of the Cardiac B-type Natriuretic Peptide Promoter.
S. S. Bhalla, L. Robitaille, and M. Nemer (2001)
J. Biol. Chem. 276, 11439-11445
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)