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Science 27 January 1989:
Vol. 243. no. 4890, pp. 533 - 535
DOI: 10.1126/science.2536194
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Articles

Science, Vol 243, Issue 4890, 533-535
Copyright © 1989 by American Association for the Advancement of Science

articles

Germline transmission of exogenous genes in the chicken

RA Bosselman, RY Hsu, T Boggs, S Hu, J Bruszewski, S Ou, L Kozar, F Martin, C Green, F Jacobsen, and al. et

Amgen Inc., Thousand Oaks, CA 91320.

Difficulties associated with in vitro manipulation and culture of the early chicken embryo have restricted generation of transgenic chickens to approaches that use replication-competent retroviruses. The need to produce transgenic chickens in the absence of replicating virus prompted development of a new method of gene transfer into the chicken. Microinjection of the replication-defective reticuloendotheliosis virus (REV) vector ME111 beneath unincubated chicken embryo blastoderms results in infection of germline stem cells. This vector contains genetic information exogenous to the chicken genome, including both the herpes simplex virus type 1 thymidine kinase gene and the Tn5 neomycin phosphotransferase gene. About 8 percent of male birds hatched from injected embryos contained vector DNA in their semen. All four positive males tested passed vector sequences onto their progeny. Analysis of G1 offspring showed that gonads of G0 male birds were mosaic with respect to insertion of vector provirus. Thus, primordial germ cells present in the unincubated chicken embryo blastoderm are susceptible to infection by defective REV vectors.


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Development of Transgenic Chickens Expressing Human Parathormone Under the Control of a Ubiquitous Promoter by Using a Retrovirus Vector System.
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Retrovirus-mediated in vitro gene transfer into chicken male germ line cells.
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The incredible, edible, and therapeutic egg.
J. N. Petitte and P. E. Mozdziak (2007)
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Generation of tissue-specific transgenic birds with lentiviral vectors.
B. B. Scott and C. Lois (2005)
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High-Level Expression of Single-Chain Fv-Fc Fusion Protein in Serum and Egg White of Genetically Manipulated Chickens by Using a Retroviral Vector.
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Ubiquitous GFP expression in transgenic chickens using a lentiviral vector.
S. C. Chapman, A. Lawson, W. C. MacArthur, R. J. Wiese, R. H. Loechel, M. Burgos-Trinidad, J. K. Wakefield, R. Ramabhadran, T. J. Mauch, and G. C. Schoenwolf (2005)
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Retrovirus vectors: promise and reality.
H. Temin (1989)
Science 246, 983
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Genetic engineering of livestock.
V. Pursel, C. Pinkert, K. Miller, D. Bolt, R. Campbell, R. Palmiter, R. Brinster, and R. Hammer (1989)
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