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Science 13 January 1989: Vol. 243. no. 4888, pp. 223 - 226 DOI: 10.1126/science.2643161
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Articles
Science, Vol 243, Issue 4888, 223-226
Copyright © 1989 by American Association for the Advancement of Science
AIDS-Kaposi's sarcoma-derived cells express cytokines with autocrine and paracrine growth effects
B Ensoli,
S Nakamura,
SZ Salahuddin,
P Biberfeld,
L Larsson,
B Beaver,
F Wong-Staal,
and
RC Gallo
Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, MD 20892.
When grown in vitro, cells from Kaposi's sarcoma lesions of AIDS patients (AIDS-KS cells) constitutively release several growth promoting activities. When inoculated into nude mice, the AIDS-KS cells induce a KS-like lesion of mouse origin. Here it is shown that the AIDS-KS cells express messenger RNA for a complex mixture of cytokines that correlate with several of the biological activities of these cells. Basic fibroblast growth factor, which is a potent angiogenic factor, and interleukin-1 messenger RNAs are expressed at very high levels and seem to account for a large proportion of the activities, since their corresponding proteins are released in biologically active form into the culture media where they induce autocrine and paracrine growth effects.
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