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Science 13 January 1989:
Vol. 243. no. 4888, pp. 217 - 220
DOI: 10.1126/science.2463672
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Articles

Science, Vol 243, Issue 4888, 217-220
Copyright © 1989 by American Association for the Advancement of Science

articles

Polymerase chain reaction with single-sided specificity: analysis of T cell receptor delta chain

EY Loh, JF Elliott, S Cwirla, LL Lanier, and MM Davis

Departments of Medicine and Microbiology and Immunology, Stanford University School of Medicine, CA 94305-5402.

In the polymerase chain reaction (PCR), two specific oligonucleotide primers are used to amplify the sequences between them. However, this technique is not suitable for amplifying genes that encode molecules where the 5' portion of the sequences of interest is not known, such as the T cell receptor (TCR) or immunoglobulins. Because of this limitation, a novel technique, anchored polymerase chain reaction (A-PCR), was devised that requires sequence specificity only on the 3' end of the target fragment. It was used to analyze TCR delta chain mRNA's from human peripheral blood gamma delta T cells. Most of these cells had a V delta gene segment not previously described (V delta 3), and the delta chain junctional sequences formed a discrete subpopulation compared with those previously reported.


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