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Science 13 January 1989:
Vol. 243. no. 4888, pp. 187 - 192
DOI: 10.1126/science.2492114
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Articles

Science, Vol 243, Issue 4888, 187-192
Copyright © 1989 by American Association for the Advancement of Science

articles

Peptide and protein synthesis by segment synthesis-condensation

ET Kaiser, H Mihara, GA Laforet, JW Kelly, L Walters, MA Findeis, and T Sasaki

Laboratory of Bioorganic Chemistry and Biochemistry, Rockefeller University, New York, NY 10021.

The chemical synthesis of biologically active peptides and polypeptides can be achieved by using a convergent strategy of condensing protected peptide segments to form the desired molecule. An oxime support increases the ease with which intermediate protected peptides can be synthesized and makes this approach useful for the synthesis of peptides in which secondary structural elements have been redesigned. The extension of these methods to large peptides and proteins, for which folding of secondary structures into functional tertiary structures is critical, is discussed. Models of apolipoproteins, the homeo domain from the developmental protein encoded by the Antennapedia gene of Drosophila, a part of the Cro repressor, and the enzyme ribonuclease T1 and a structural analog have been synthesized with this method.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Binding of Cationic alpha -Helical Peptides to Plasmid DNA and Their Gene Transfer Abilities into Cells.
T. Niidome, N. Ohmori, A. Ichinose, A. Wada, H. Mihara, T. Hirayama, and H. Aoyagi (1997)
J. Biol. Chem. 272, 15307-15312
   Abstract »    Full Text »    PDF »
Peptide synthesis catalyzed by an antibody containing a binding site for variable amino acids.
R Hirschmann, A. Smith 3rd, C. Taylor, P. Benkovic, S. Taylor, K. Yager, P. Sprengeler, and S. Benkovic (1994)
Science 265, 234-237
   Abstract »    PDF »
Enzymatic catalysts in organic synthesis.
C. Wong (1989)
Science 244, 1145-1152
   Abstract »    PDF »


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