Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 23 December 1988:
Vol. 242. no. 4886, pp. 1706 - 1709
DOI: 10.1126/science.2904703

Articles

Science, Vol 242, Issue 4886, 1706-1709
Copyright © 1988 by American Association for the Advancement of Science


articles

Engraftment of immune-deficient mice with human hematopoietic stem cells

S Kamel-Reid and JE Dick

Department of Genetics, Hospital for Sick Children, Toronto, Ontario.

A system in which immune-deficient mice are repopulated with cells from the human myeloid lineage, and that provides an in vivo stem cell assay for human hematopoietic cells is described. Generation of the chimeric human/immune-deficient (HID) mice was dependent on the use of immune-deficient bg/nu/xid mice. Infusion of these mice with human bone marrow gave rise to increases in human macrophage progenitors during more than 5 weeks of in vivo growth, indicating the seeding, proliferation, and differentiation of human stem cells. The human identity of the progenitors was confirmed by sequence analysis and their dependence on human growth factors. The creation of HID mice lays the foundation for establishing animal models for a wide variety of human hemopathies, from leukemia to infectious disease.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Stem cell concepts renew cancer research.
J. E. Dick (2008)
Blood 112, 4793-4807
   Abstract »    Full Text »    PDF »
Broad influenza-specific CD8+ T-cell responses in humanized mice vaccinated with influenza virus vaccines.
C. I. Yu, M. Gallegos, F. Marches, G. Zurawski, O. Ramilo, A. Garcia-Sastre, J. Banchereau, and A. K. Palucka (2008)
Blood 112, 3671-3678
   Abstract »    Full Text »    PDF »
Timing of neutrophil tissue repopulation predicts restoration of innate immune protection in a murine bone marrow transplantation model.
C. Cheretakis, R. Leung, C. X. Sun, Y. Dror, and M. Glogauer (2006)
Blood 108, 2821-2826
   Abstract »    Full Text »    PDF »
Experimental Models to Study Development and Function of the Human Immune System In Vivo.
N. Legrand, K. Weijer, and H. Spits (2006)
J. Immunol. 176, 2053-2058
   Abstract »    Full Text »    PDF »
Anti-human CTLA-4 monoclonal antibody promotes T-cell expansion and immunity in a hu-PBL-SCID model: a new method for preclinical screening of costimulatory monoclonal antibodies.
K. F. May Jr, S. Roychowdhury, D. Bhatt, E. Kocak, X.-F. Bai, J.-Q. Liu, A. K. Ferketich, E. W. Martin Jr, M. A. Caligiuri, P. Zheng, et al. (2005)
Blood 105, 1114-1120
   Abstract »    Full Text »    PDF »
Combination Immunotherapy of B-Cell Non-Hodgkin's Lymphoma with Rituximab and Interleukin-2: A Preclinical and Phase I Study.
C. F. Eisenbeis, A. Grainger, B. Fischer, R. A. Baiocchi, L. Carrodeguas, S. Roychowdhury, L. Chen, A. L. Banks, T. Davis, D. Young, et al. (2004)
Clin. Cancer Res. 10, 6101-6110
   Abstract »    Full Text »    PDF »
Improved method to bridge mouse and man.
J. Nolta (2004)
Blood 103, 370-371
   Full Text »    PDF »
Lentivector-mediated clonal tracking reveals intrinsic heterogeneity in the human hematopoietic stem cell compartment and culture-induced stem cell impairment.
F. Mazurier, O. I. Gan, J. L. McKenzie, M. Doedens, and J. E. Dick (2004)
Blood 103, 545-552
   Abstract »    Full Text »    PDF »
Treatment of Malignant Gliomas with a Replicating Adenoviral Vector Expressing Herpes Simplex Virus-Thymidine Kinase.
D. Nanda, R. Vogels, M. Havenga, C. J. Avezaat, A. Bout, and P. S. Smitt (2001)
Cancer Res. 61, 8743-8750
   Abstract »    Full Text »    PDF »
A leukemic stem cell with intrinsic drug efflux capacity in acute myeloid leukemia.
G. G. Wulf, R.-Y. Wang, I. Kuehnle, D. Weidner, F. Marini, M. K. Brenner, M. Andreeff, and M. A. Goodell (2001)
Blood 98, 1166-1173
   Abstract »    Full Text »    PDF »
Xenotransplantation of immunodeficient mice with mobilized human blood CD34+ cells provides an in vivo model for human megakaryocytopoiesis and platelet production.
L. E. Perez, H. M. Rinder, C. Wang, J. B. Tracey, N. Maun, and D. S. Krause (2001)
Blood 97, 1635-1643
   Abstract »    Full Text »    PDF »
Acute Myeloid Leukemia.
F. R. Appelbaum, J. M. Rowe, J. Radich, and J. E. Dick (2001)
Hematology 2001, 62-86
   Abstract »    Full Text »    PDF »
Human hematopoietic stem cells stimulated to proliferate in vitro lose engraftment potential during their S/G2/M transit and do not reenter G0.
H. Glimm, I.-H. Oh, and C. J. Eaves (2000)
Blood 96, 4185-4193
   Abstract »    Full Text »    PDF »
Highly efficient gene transfer into cord blood nonobese diabetic/severe combined immunodeficiency repopulating cells by oncoretroviral vector particles pseudotyped with the feline endogenous retrovirus (RD114) envelope protein.
P. F. Kelly, J. Vandergriff, A. Nathwani, A. W. Nienhuis, and E. F. Vanin (2000)
Blood 96, 1206-1214
   Abstract »    Full Text »    PDF »
Quantitative assessment of retroviral transfer of the human multidrug resistance 1 gene to human mobilized peripheral blood progenitor cells engrafted in nonobese diabetic/severe combined immunodeficient mice.
B. Schiedlmeier, K. Kuhlcke, H. G. Eckert, C. Baum, W. J. Zeller, and S. Fruehauf (2000)
Blood 95, 1237-1248
   Abstract »    Full Text »    PDF »
Expansion of human cord blood CD34+CD38- cells in ex vivo culture during retroviral transduction without a corresponding increase in SCID repopulating cell (SRC) frequency: dissociation of SRC phenotype and function.
C. Dorrell, O. I. Gan, D. S. Pereira, R. G. Hawley, and J. E. Dick (2000)
Blood 95, 102-110
   Abstract »    Full Text »    PDF »
Differentiation Stage-Specific Regulation of Primitive Human Hematopoietic Progenitor Cycling by Exogenous and Endogenous Inhibitors in an In Vivo Model.
J.D. Cashman, I. Clark-Lewis, A.C. Eaves, and C.J. Eaves (1999)
Blood 94, 3722-3729
   Abstract »    Full Text »    PDF »
Human Hematopoietic Stem/Progenitor Cells Generate CD5+ B Lymphoid Cells in NOD/SCID Mice.
E. M. Novelli, M. Ramírez, W. Leung, and C. I. Civin (1999)
Stem Cells 17, 242-252
   Abstract »    Full Text »
Long-Term Ex Vivo Maintenance and Expansion of Transplantable Human Hematopoietic Stem Cells.
C.-C. Shih, M. C.-T. Hu, J. Hu, J. Medeiros, and S. J. Forman (1999)
Blood 94, 1623-1636
   Abstract »    Full Text »    PDF »
Novel Endogenous Type D Retroviral Particles Expressed at High Levels in a SCID Mouse Thymic Lymphoma.
S. Ristevski, D. F. J. Purcell, J. Marshall, D. Campagna, S. Nouri, S. P. Fenton, D. A. McPhee, and G. Kannourakis (1999)
J. Virol. 73, 4662-4669
   Abstract »    Full Text »
Engraftment in Nonobese Diabetic Severe Combined Immunodeficient Mice of Human CD34+ Cord Blood Cells After Ex Vivo Expansion: Evidence for the Amplification and Self-Renewal of Repopulating Stem Cells.
W. Piacibello, F. Sanavio, A. Severino, A. Dane, L. Gammaitoni, F. Fagioli, E. Perissinotto, G. Cavalloni, O. Kollet, T. Lapidot, et al. (1999)
Blood 93, 3736-3749
   Abstract »    Full Text »    PDF »
One-Day Ex Vivo Culture Allows Effective Gene Transfer Into Human Nonobese Diabetic/Severe Combined Immune-Deficient Repopulating Cells Using High-Titer Vesicular Stomatitis Virus G Protein Pseudotyped Retrovirus.
V. I. Rebel, M. Tanaka, J.-S. Lee, S. Hartnett, M. Pulsipher, D. G. Nathan, R. C. Mulligan, and C. A. Sieff (1999)
Blood 93, 2217-2224
   Abstract »    Full Text »    PDF »
High Efficiency Gene Transfer to Human Hematopoietic SCID-Repopulating Cells Under Serum-Free Conditions.
A. J. Schilz, G. Brouns, O. G. Ottmann, D. Hoelzer, A. A. Fauser, A. J. Thrasher, and M. Grez (1998)
Blood 92, 3163-3171
   Abstract »    Full Text »    PDF »
Long-Term Immune Reconstitution and Outcome After HLA-Nonidentical T-Cell-Depleted Bone Marrow Transplantation for Severe Combined Immunodeficiency: A European Retrospective Study of 116 Patients.
E. Haddad, P. Landais, W. Friedrich, B. Gerritsen, M. Cavazzana-Calvo, G. Morgan, Y. Bertrand, A. Fasth, F. Porta, A. Cant, et al. (1998)
Blood 91, 3646-3653
   Abstract »    Full Text »    PDF »
Biology of Human Umbilical Cord Blood-Derived Hematopoietic Stem/Progenitor Cells.
H. Mayani and P. M. Lansdorp (1998)
Stem Cells 16, 153-165
   Abstract »    Full Text »
SCID Mouse Models of Human Stem Cell Engraftment.
D. L. Greiner, R. A. Hesselton, and L. D. Shultz (1998)
Stem Cells 16, 166-177
   Abstract »    Full Text »
Transplantation of Human Umbilical Cord Blood Cells in Macrophage-Depleted SCID Mice: Evidence for Accessory Cell Involvement in Expansion of Immature CD34+CD38- Cells.
M. M.A. Verstegen, P. B. van Hennik, W. Terpstra, C. van den Bos, J. J. Wielenga, N. van Rooijen, R. E. Ploemacher, G. Wagemaker, and A. W. Wognum (1998)
Blood 91, 1966-1976
   Abstract »    Full Text »    PDF »
Inhibition of Granulocyte-Macrophage Colony-Stimulating Factor Prevents Dissemination and Induces Remission of Juvenile Myelomonocytic Leukemia in Engrafted Immunodeficient Mice.
P. O. Iversen, I. D. Lewis, S. Turczynowicz, H. Hasle, C. Niemeyer, K. Schmiegelow, S. Bastiras, A. Biondi, T. P. Hughes, and A. F. Lopez (1997)
Blood 90, 4910-4917
   Abstract »    Full Text »    PDF »
Phenotypic and Functional Analysis of Fas (CD95) Expression in Primary Central Nervous System Lymphoma of Patients With Acquired Immunodeficiency Syndrome.
R. A. Baiocchi, V. P. Khatri, M. J. Lindemann, M. E. Ross, G. Papoff, A. J. Caprio, T. V. Caprio, R. Fenstermaker, G. Ruberti, Z. P. Bernstein, et al. (1997)
Blood 90, 1737-1746
   Abstract »    Full Text »    PDF »
Long-Term Malignant Hematopoiesis in Human Acute Leukemia Bone Marrow Biopsies Implanted in Severe Combined Immunodeficiency Mice.
F. Legrand, I. Khazaal, M. Peuchmaur, O. Fenneteau, H. Cave, P. Rohrlich, E. Vilmer, and B. Peault (1997)
Blood 90, 2001-2009
   Abstract »    Full Text »    PDF »
Normal Immunologic Response to a Neoantigen, Bacteriophage Phi X-174, in Baboons With Long-Term Lymphohematopoietic Reconstitution From Highly Purified CD34+ Lin- Allogeneic Marrow Cells.
R. G. Andrews, A. Winkler, J. Potter, E. Bryant, G. H. Knitter, I. D. Bernstein, and H. D. Ochs (1997)
Blood 90, 1701-1708
   Abstract »    Full Text »    PDF »
Growth of Human Hematopoietic Cells in Immunodeficient Mice Conditioned with Cyclophosphamide and Busulfan.
R. S. Basch, F. L. Quito, J. Beh, and J. A. Hirst (1997)
Stem Cells 15, 314-323
   Abstract »    Full Text »
Engraftment and Development of Human CD34+-Enriched Cells From Umbilical Cord Blood in NOD/LtSz-scid/scid Mice.
C. J. Hogan, E. J. Shpall, O. McNulty, I. McNiece, J. E. Dick, L. D. Shultz, and G. Keller (1997)
Blood 90, 85-96
   Abstract »    Full Text »    PDF »
Kinetic Evidence of the Regeneration of Multilineage Hematopoiesis From Primitive Cells in Normal Human Bone Marrow Transplanted Into Immunodeficient Mice.
J. D. Cashman, T. Lapidot, J. C.Y. Wang, M. Doedens, L. D. Shultz, P. Lansdorp, J. E. Dick, and C. J. Eaves (1997)
Blood 89, 4307-4316
   Abstract »    Full Text »    PDF »
Cytokine stimulation of multilineage hematopoiesis from immature human cells engrafted in SCID mice.
T Lapidot, F Pflumio, M Doedens, B Murdoch, D. Williams, and J. Dick (1992)
Science 255, 1137-1141
   Abstract »    PDF »
Engraftment and development of human T and B cells in mice after bone marrow transplantation.
I Lubin, Y Faktorowich, T Lapidot, Y Gan, Z Eshhar, E Gazit, M Levite, and Y Reisner (1991)
Science 252, 427-431
   Abstract »    PDF »
An in vivo model of somatic cell gene therapy for human severe combined immunodeficiency.
G Ferrari, S Rossini, R Giavazzi, D Maggioni, N Nobili, M Soldati, G Ungers, F Mavilio, E Gilboa, and C Bordignon (1991)
Science 251, 1363-1366
   Abstract »    PDF »
Relative Contributions of Host and Microbial Factors in Bacterial Translocation.
C. L. Wells, R. P. Jechorek, and K. J. Gillingham (1991)
Arch Surg 126, 247-252
   Abstract »    PDF »
Correction of CD18-deficient lymphocytes by retrovirus-mediated gene transfer.
J. Wilson, A. Ping, J. Krauss, L Mayo-Bond, C. Rogers, D. Anderson, and R. Todd (1990)
Science 248, 1413-1416
   Abstract »    PDF »
A model of human acute lymphoblastic leukemia in immune-deficient SCID mice.
S Kamel-Reid, M Letarte, C Sirard, M Doedens, T Grunberger, G Fulop, M. Freedman, R. Phillips, and J. Dick (1989)
Science 246, 1597-1600
   Abstract »    PDF »
ANIMAL MODELS FOR AIDS.
(1989)
Journal Watch (General) 1989, 4
   Full Text »



To Advertise     Find Products


Science. ISSN 0036-8075 (print), 1095-9203 (online)