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Science 9 December 1988:
Vol. 242. no. 4884, pp. 1446 - 1448
DOI: 10.1126/science.2904699
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Articles

Science, Vol 242, Issue 4884, 1446-1448
Copyright © 1988 by American Association for the Advancement of Science

articles

Metabolic correction of defects in the lipid anchoring of Thy-1 in lymphoma mutants

D Gupta, A Tartakoff, and E Tisdale

Department of Pathology, Case Western Reserve University, Cleveland, OH 44106.

Many plasma membrane proteins, including Thy-1, are anchored by a carboxyl terminal glycophospholipid. This unit is absent from the Thy-1 of several lymphoma mutants that synthesize the Thy-1 polypeptide but fail to express it at the cell surface. Recessive mutants of complementation groups A to C, E, and F contain Thy-1 mRNA of normal size, which suggests that their Thy-1 polypeptide is normal. To identify possible metabolic lesions, each mutant was grown with various supplements. The class F and B mutants exhibited a reversible induction of surface lipid anchored Thy-1 when grown with the aminoglycoside G418. Other aminoglycosides, sugars, and ethanolamine were inactive. These unexpected observations are discussed in the context of lipid anchor biosynthesis.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Ethanolamine Phosphate Linked to the First Mannose Residue of Glycosylphosphatidylinositol (GPI) Lipids Is a Major Feature of the GPI Structure That Is Recognized by Human GPI Transamidase.
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Down-Regulation of Glycosylphosphatidylinositol-Specific Phospholipase D Induced by Lipopolysaccharide and Oxidative Stress in the Murine Monocyte- Macrophage Cell Line RAW 264.7.
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Phosphatidylinositol Phospholipase C Is Activated Allosterically by the Aminoglycoside G418. 2-DEOXY-2-FLUORO-SCYLLO-INOSITOL-1-O-DODECYLPHOSPHONATE AND ITS ANALOGS INHIBIT GLYCOSYLPHOSPHATIDYLINOSITOL PHOSPHOLIPASE C.
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Glycan Requirements of Glycosylphosphatidylinositol Phospholipase C from Trypanosoma brucei.
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Correction of a defect in mammalian GPI anchor biosynthesis by a transfected yeast gene.
R DeGasperi, L. Thomas, E Sugiyama, H. Chang, P. Beck, P Orlean, C Albright, G Waneck, J. Sambrook, C. Warren, et al. (1990)
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