Related Content
Search Google Scholar for:
|
|
Science 2 December 1988:
Vol. 242. no. 4883, pp. 1306 - 1308
DOI: 10.1126/science.2848320
|
|
Articles
Science, Vol 242, Issue 4883, 1306-1308
Copyright © 1988 by American Association for the Advancement of Science
Transient expression shows ligand gating and allosteric potentiation of GABAA receptor subunits
DB Pritchett,
H Sontheimer,
CM Gorman,
H Kettenmann,
PH Seeburg,
and
PR Schofield
Laboratory of Molecular Neuroendocrinology, ZMBH, University of Heidelberg, Federal Republic of Germany.
Human gamma-aminobutyric acid A (GABAA) receptor subunits were expressed transiently in cultured mammalian cells. This expression system allows the simultaneous characterization of ligand-gated ion channels by electrophysiology and by pharmacology. Thus, coexpression of the alpha and beta subunits of the GABAA receptor generated GABA-gated chloride channels and binding sites for GABAA receptor ligands. Channels consisting of only alpha or beta subunits could also be detected. These homomeric channels formed with reduced efficiencies compared to the heteromeric receptors. Both of these homomeric GABA-responsive channels were potentiated by barbiturate, indicating that sites for both ligand-gating and allosteric potentiation are present on receptors assembled from either subunit.
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- Functional role of TRPC proteins in native systems: implications from knockout and knock-down studies.
- M. Freichel, R. Vennekens, J. Olausson, S. Stolz, S. E Philipp, P. Weissgerber, and V. Flockerzi (2005)
J. Physiol.
567, 59-66
| Abstract »
| Full Text »
| PDF »
- A TM2 Residue in the {beta}1 Subunit Determines Spontaneous Opening of Homomeric and Heteromeric {gamma}-Aminobutyric Acid-gated Ion Channels.
- A. Miko, E. Werby, H. Sun, J. Healey, and L. Zhang (2004)
J. Biol. Chem.
279, 22833-22840
| Abstract »
| Full Text »
| PDF »
- Tracazolate Reveals a Novel Type of Allosteric Interaction with Recombinant gamma -Aminobutyric AcidA Receptors.
- S.-A. Thompson, P. B. Wingrove, L. Connelly, P. J. Whiting, and K. A. Wafford (2002)
Mol. Pharmacol.
61, 861-869
| Abstract »
| Full Text »
| PDF »
- Mutating the Highly Conserved Second Membrane-Spanning Region 9' Leucine Residue in the alpha 1 or beta 1 Subunit Produces Subunit-Specific Changes in the Function of Human alpha 1beta 1 gamma -Aminobutyric AcidA Receptors.
- J. E. Dalziel, G. B. Cox, P. W. Gage, and B. Birnir (2000)
Mol. Pharmacol.
57, 875-882
| Abstract »
| Full Text »
- Agonist Gating and Isoflurane Potentiation in the Human gamma -Aminobutyric Acid Type A Receptor Determined by the Volume of a Second Transmembrane Domain Residue.
- V. V. Koltchine, S. E. Finn, A. Jenkins, N. Nikolaeva, A. Lin, and N. L. Harrison (1999)
Mol. Pharmacol.
56, 1087-1093
| Abstract »
| Full Text »
- Expression and Trans-synaptic Regulation of P2x4 and P2z Receptors for Extracellular ATP in Parotid Acinar Cells. EFFECTS OF PARASYMPATHETIC DENERVATION.
- L. Tenneti, S. J. Gibbons, and B. R. Talamo (1998)
J. Biol. Chem.
273, 26799-26808
| Abstract »
| Full Text »
| PDF »
- Residues at Positions 206 and 209 of the alpha 1 Subunit of gamma -Aminobutyric AcidA Receptors Influence Affinities for Benzodiazepine Binding Site Ligands.
- A. Buhr, M. T. Schaerer, R. Baur, and E. Sigel (1997)
Mol. Pharmacol.
52, 676-682
| Abstract »
| Full Text »
- Assembly of GABAA Receptors Composed of alpha 1 and beta 2 Subunits in Both Cultured Neurons and Fibroblasts.
- G. H. Gorrie, Y. Vallis, A. Stephenson, J. Whitfield, B. Browning, T. G. Smart, and S. J. Moss (1997)
J. Neurosci.
17, 6587-6596
| Abstract »
| Full Text »
| PDF »
- An Amino Acid Substitution in the Pore Region of a Glutamate-gated Chloride Channel Enables the Coupling of Ligand Binding to Channel Gating.
- A. Etter, D. F. Cully, J. M. Schaeffer, K. K. Liu, and J. P. Arena (1996)
J. Biol. Chem.
271, 16035-16039
| Abstract »
| Full Text »
| PDF »
- Assembly and Cell Surface Expression of Heteromeric and Homomeric [IMAGE]-Aminobutyric Acid Type A Receptors.
- C. N. Connolly, B. J. Krishek, B. J. McDonald, T. G. Smart, and S. J. Moss (1996)
J. Biol. Chem.
271, 89-96
| Abstract »
| Full Text »
| PDF »
- Functional modulation of GABAA receptors by cAMP-dependent protein phosphorylation.
- S. Moss, T. Smart, C. Blackstone, and R. Huganir (1992)
Science
257, 661-665
| Abstract »
| PDF »
- Identification of a specialized adenylyl cyclase that may mediate odorant detection.
- H. Bakalyar and R. Reed (1990)
Science
250, 1403-1406
| Abstract »
| PDF »
- Type I and type II GABAA-benzodiazepine receptors produced in transfected cells.
- D. Pritchett, H Luddens, and P. Seeburg (1989)
Science
245, 1389-1392
| Abstract »
| PDF »
|
|
