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Science 25 November 1988:
Vol. 242. no. 4882, pp. 1168 - 1171
DOI: 10.1126/science.2460924
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Articles

Science, Vol 242, Issue 4882, 1168-1171
Copyright © 1988 by American Association for the Advancement of Science

articles

Fidelity of HIV-1 reverse transcriptase

BD Preston, BJ Poiesz, and LA Loeb

Department of Pathology, University of Washington, Seattle 98195.

The human immunodeficiency virus type 1 (HIV-1) shows extensive genetic variation and undergoes rapid evolution. The fidelity of purified HIV-1 reverse transcriptase was measured during DNA polymerization in vitro by means of three different assays. Reverse transcriptase from HIV-1 introduced base-substitution errors in DNA from the bacteriophage phi X174 amber3 at estimated frequencies of 1/2000 to 1/4000. Analyses of misincorporation rates opposite a single template adenine residue showed that HIV-1 reverse transcriptase catalyzed nucleotide mismatches with a specificity of A:C much greater than A:G greater than A:A. The high error rate of HIV-1 reverse transcriptase in vitro translates to approximately five to ten errors per HIV-1 genome per round of replication in vivo. This high error rate suggests that misincorporation by HIV-1 reverse transcriptase is, at least in part, responsible for the hypermutability of the AIDS virus. The specificity of misincorporation may provide a basis for the systematic construction of antiviral nucleosides.


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V. R. K. Yedavalli, C. Chappey, E. Matala, and N. Ahmad (1998)
J. Virol. 72, 1092-1102
   Abstract »    Full Text »    PDF »
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Low Fidelity Mutants in the O-Helix of Thermus aquaticus DNA Polymerase I.
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N. Engl. J. Med. 333, 1331-1339
   Full Text »    PDF »
Enzymatic Characterization of Human Immunodeficiency Virus Type 1 Reverse Transcriptase Resistant to Multiple 2`,3`-Dideoxynucleoside 5`-Triphosphates.
T. Ueno, T. Shirasaka, and H. Mitsuya (1995)
J. Biol. Chem. 270, 23605-23611
   Abstract »    Full Text »    PDF »
Reduced Frameshift Fidelity and Processivity of HIV-1 Reverse Transcriptase Mutants Containing Alanine Substitutions in Helix H of the Thumb Subdomain.
K. Bebenek, W. A. Beard, J. R. Casas-Finet, H.-R. Kim, T. A. Darden, S. H. Wilson, and T. A. Kunkel (1995)
J. Biol. Chem. 270, 19516-19523
   Abstract »    Full Text »    PDF »
Time to Hit HIV, Early and Hard.
D. D. Ho (1995)
N. Engl. J. Med. 333, 450-451
   Full Text »
Influence of Human Immunodeficiency Virus Nucleocapsid Protein on Synthesis and Strand Transfer by the Reverse Transcriptase in Vitro.
L. Rodrguez-Rodrguez, Z. Tsuchihashi, G. M. Fuentes, R. A. Bambara, and P. J. Fay (1995)
J. Biol. Chem. 270, 15005-15011
   Abstract »    Full Text »    PDF »
Mechanism of DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase.
J. Peliska and S. Benkovic (1992)
Science 258, 1112-1118
   Abstract »    PDF »
Crystal structure at 3.5 A resolution of HIV-1 reverse transcriptase complexed with an inhibitor.
L. Kohlstaedt, J Wang, J. Friedman, P. Rice, and T. Steitz (1992)
Science 256, 1783-1790
   Abstract »    PDF »
Selective transmission of human immunodeficiency virus type-1 variants from mothers to infants.
S. Wolinsky, C. Wike, B. Korber, C Hutto, W. Parks, L. Rosenblum, K. Kunstman, M. Furtado, and J. Munoz (1992)
Science 255, 1134-1137
   Abstract »    PDF »
Antigenic diversity thresholds and the development of AIDS.
M. Nowak, R. Anderson, A. McLean, T. Wolfs, J Goudsmit, and R. May (1991)
Science 254, 963-969
   Abstract »    PDF »
DNA polymerase fidelity and the polymerase chain reaction..
K A Eckert and T A Kunkel (1991)
Genome Res. 1, 17-24
   Abstract »    PDF »
Antiretroviral Therapy in AIDS.
S. Broder, H. Mitsuya, R. Yarchoan, and G. N. Pavlakis (1990)
Ann Intern Med 113, 604-618
   Abstract »    PDF »
Molecular targets for AIDS therapy.
H Mitsuya, R Yarchoan, and S Broder (1990)
Science 249, 1533-1544
   Abstract »    PDF »
Multiple mutations in HIV-1 reverse transcriptase confer high-level resistance to zidovudine (AZT).
B. Larder and S. Kemp (1989)
Science 246, 1155-1158
   Abstract »    PDF »
Vertical-scanning Mutagenesis of a Critical Tryptophan in the "Minor Groove Binding Track" of HIV-1 Reverse Transcriptase. MAJOR GROOVE DNA ADDUCTS IDENTIFY SPECIFIC PROTEIN INTERACTIONS IN THE MINOR GROOVE.
G. J. Latham, E. Forgacs, W. A. Beard, R. Prasad, K. Bebenek, T. A. Kunkel, S. H. Wilson, and R. S. Lloyd (2000)
J. Biol. Chem. 275, 15025-15033
   Abstract »    Full Text »    PDF »
Differential Influence of Nucleoside Analog-resistance Mutations K65R and L74V on the Overall Mutation Rate and Error Specificity of Human Immunodeficiency Virus Type 1 Reverse Transcriptase.
F. S. Shah, K. A. Curr, M. E. Hamburgh, M. Parniak, H. Mitsuya, J. G. Arnez, and V. R. Prasad (2000)
J. Biol. Chem. 275, 27037-27044
   Abstract »    Full Text »    PDF »
A Single Highly Mutable Catalytic Site Amino Acid Is Critical for DNA Polymerase Fidelity.
P. H. Patel, H. Kawate, E. Adman, M. Ashbach, and L. A. Loeb (2001)
J. Biol. Chem. 276, 5044-5051
   Abstract »    Full Text »    PDF »
Analysis of Efficiency and Fidelity of HIV-1 (+)-Strand DNA Synthesis Reveals a Novel Rate-limiting Step during Retroviral Reverse Transcription.
M. Gotte, M. Kameoka, N. McLellan, L. Cellai, and M. A. Wainberg (2001)
J. Biol. Chem. 276, 6711-6719
   Abstract »    Full Text »    PDF »
Identification of a Simian Immunodeficiency Virus Reverse Transcriptase Variant with Enhanced Replicational Fidelity in the Late Stage of Viral Infection.
T. L. Diamond, J. Kimata, and B. Kim (2001)
J. Biol. Chem. 276, 23624-23631
   Abstract »    Full Text »    PDF »


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