Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Keystone Symposium

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 18 November 1988:
Vol. 242. no. 4881, pp. 1056 - 1058
DOI: 10.1126/science.2461587
Prev | Table of Contents | Next

Articles

Science, Vol 242, Issue 4881, 1056-1058
Copyright © 1988 by American Association for the Advancement of Science

articles

Activation of developmentally mutated human globin genes by cell fusion

T Papayannopoulou, T Enver, S Takegawa, NP Anagnou, and G Stamatoyannopoulos

Division of Hematology, University of Washington, Seattle 98195.

Human fetal globin genes are not expressed in hybrid cells produced by the fusion of normal human lymphocytes with mouse erythroleukemia cells. In contrast, when lymphocytes from persons with globin gene developmental mutations (hereditary persistence of fetal hemoglobin) are used for these fusions, fetal globin is expressed in the hybrid cells. Thus, mutations of developmental origin can be reconstituted in vitro by fusing mutant lymphoid cells with differentiated cell lines of the proper lineage. This system can readily be used for analyses, such as globin gene methylation, that normally require large numbers of pure nucleated erythroid cells, which are difficult to obtain.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Persistent {gamma}-globin expression in adult transgenic mice is mediated by HPFH-2, HPFH-3, and HPFH-6 breakpoint sequences.
E. Z. Katsantoni, A. Langeveld, A. W. K. Wai, D. Drabek, F. Grosveld, N. P. Anagnou, and J. Strouboulis (2003)
Blood 102, 3412-3419
   Abstract »    Full Text »    PDF »
Comparison of Expression of Human Globin Genes Transferred Into Mouse Erythroleukemia Cells and in Transgenic Mice.
E. Skarpidi, G. Vassilopoulos, G. Stamatoyannopoulos, and Q. Li (1998)
Blood 92, 3416-3421
   Abstract »    Full Text »    PDF »
A deletion of the human beta-globin locus activation region causes a major alteration in chromatin structure and replication across the entire beta-globin locus..
W C Forrester, E Epner, M C Driscoll, T Enver, M Brice, T Papayannopoulou, and M Groudine (1990)
Genes & Dev. 4, 1637-1649
   Abstract »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)