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Science 18 November 1988:
Vol. 242. no. 4881, pp. 1047 - 1050
DOI: 10.1126/science.3194756
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Articles

Science, Vol 242, Issue 4881, 1047-1050
Copyright © 1988 by American Association for the Advancement of Science

articles

A bitter substance induces a rise in intracellular calcium in a subpopulation of rat taste cells

MH Akabas, J Dodd, and Q Al-Awqati

Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032.

The sense of taste permits animals to discriminate between foods that are safe and those that are toxic. Because most poisonous plant alkaloids are intensely bitter, bitter taste warns animals of potentially hazardous foods. To investigate the mechanism of bitter taste transduction, a preparation of dissociated rat taste cells was developed that can be studied with techniques designed for single-cell measurements. Denatonium, a very bitter substance, caused a rise in the intracellular calcium concentration due to release from internal stores in a small subpopulation of taste cells. Thus, the transduction of bitter taste may occur via a receptor-second messenger mechanism leading to neurotransmitter release and may not involve depolarization-mediated calcium entry.


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Mediation of responses to calcium in taste cells by modulation of a potassium conductance.
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Science. ISSN 0036-8075 (print), 1095-9203 (online)