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Science 18 November 1988:
Vol. 242. no. 4881, pp. 1045 - 1047
DOI: 10.1126/science.3194755
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Articles

Science, Vol 242, Issue 4881, 1045-1047
Copyright © 1988 by American Association for the Advancement of Science

articles

Autologous peptides constitutively occupy the antigen binding site on Ia

S Buus, A Sette, SM Colon, and HM Grey

Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.

Low molecular weight material associated with affinity-purified class II major histocompatibility complex (MHC) molecules of mouse (Ia) had the expected properties of peptides bound to the antigen binding site of Ia. Thus, the low molecular weight material derived from the I-Ad isotype was efficient in inhibiting the binding of 125I-labeled I-Ad-specific peptide to I-Ad, but did not significantly inhibit the binding of an I-Ed-specific peptide to I-Ed; the reciprocal isotype-specific inhibition was demonstrated with low molecular weight material derived from I-Ed. The inhibitory material was predominantly peptide in nature, as shown by its susceptibility to protease digestion. It was heterogeneous as measured by gel filtration (mean molecular weight approximately 3000), and when characterized by high-performance liquid chromatography, it eluted over a wide concentration of solvent. Such self peptide-MHC complexes may have broad significance in the biology of T cell responses, including generation of the T cell repertoire, the specificity of mixed lymphocyte responses, and the immune surveillance of self and nonself antigens in peripheral lymphoid tissues.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Chemical Features of Peptide Selection by the Class II Histocompatibility Molecules.
E. R. Unanue (1999)
Am. J. Pathol. 154, 651-664
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Initiation of the Autologous Mixed Lymphocyte Reaction Requires the Expression of Costimulatory Molecules B7-1 and B7-2 on Human Peripheral Blood Dendritic Cells.
C. Scheinecker, K. P. Machold, O. Majdic, P. Hocker, W. Knapp, and J. S. Smolen (1998)
J. Immunol. 161, 3966-3973
   Abstract »    Full Text »    PDF »
Recognition by class II alloreactive T cells of processed determinants from human serum proteins.
P Panina-Bordignon, G Corradin, E Roosnek, A Sette, and A Lanzavecchia (1991)
Science 252, 1548-1550
   Abstract »    PDF »
Limit of T cell tolerance to self proteins by peptide presentation.
H Schild, O Rotzschke, H Kalbacher, and H. Rammensee (1990)
Science 247, 1587-1589
   Abstract »    PDF »
Vaccination against experimental allergic encephalomyelitis with T cell receptor peptides.
M. Howell, S. Winters, T Olee, H. Powell, D. Carlo, and S. Brostoff (1989)
Science 246, 668-670
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Science. ISSN 0036-8075 (print), 1095-9203 (online)