Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 7 October 1988:
Vol. 242. no. 4875, pp. 93 - 97
DOI: 10.1126/science.3051379
Prev | Table of Contents | Next

Articles

Science, Vol 242, Issue 4875, 93-97
Copyright © 1988 by American Association for the Advancement of Science

articles

tRNAi(met) functions in directing the scanning ribosome to the start site of translation

AM Cigan, L Feng, and TF Donahue

Department of Molecular Biology, Northwestern University Medical School, Chicago, IL 60611.

The mechanism by which the scanning ribosome recognizes the first AUG codon nearest the 5' end of eukaryotic messenger RNA has not been established. To investigate this an anticodon change (3'-UCC-5') was introduced into one of the four methionine initiator (tRNAi(met) genes of Saccharomyces cerevisiae. The ability of the mutant transfer RNA to restore growth properties to his4 initiator codon mutant yeast strains in the absence of histidine was then assayed. Only the complementary codon, AGG, at the his4 initiator region supported His+ growth. The mutant transfer RNA also directed the ribosome to initiate at an AGG placed in the upstream region of the his4 message. Initiation at this upstream AGG precluded initiation at a downstream AGG in accordance with the "scanning" model. Therefore, an anticodon: codon interaction between tRNAi(met) as part of the scanning ribosome and the first AUG must function in directing the ribosome to the eukaryotic initiator region.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Kinetic and thermodynamic analysis of the role of start codon/anticodon base pairing during eukaryotic translation initiation.
S. E. Kolitz, J. E. Takacs, and J. R. Lorsch (2009)
RNA 15, 138-152
   Abstract »    Full Text »    PDF »
The naturally occurring N6-threonyl adenine in anticodon loop of Schizosaccharomyces pombe tRNAi causes formation of a unique U-turn motif.
E. Lescrinier, K. Nauwelaerts, K. Zanier, K. Poesen, M. Sattler, and P. Herdewijn (2006)
Nucleic Acids Res. 34, 2878-2886
   Abstract »    Full Text »    PDF »
Specific functional interactions of nucleotides at key -3 and +4 positions flanking the initiation codon with components of the mammalian 48S translation initiation complex..
A. V. Pisarev, V. G. Kolupaeva, V. P. Pisareva, W. C. Merrick, C. U.T. Hellen, and T. V. Pestova (2006)
Genes & Dev. 20, 624-636
   Abstract »    Full Text »    PDF »
HFA1 Encoding an Organelle-specific Acetyl-CoA Carboxylase Controls Mitochondrial Fatty Acid Synthesis in Saccharomyces cerevisiae.
U. Hoja, S. Marthol, J. Hofmann, S. Stegner, R. Schulz, S. Meier, E. Greiner, and E. Schweizer (2004)
J. Biol. Chem. 279, 21779-21786
   Abstract »    Full Text »    PDF »
Localization of a promoter in the putative internal ribosome entry site of the Saccharomyces cerevisiae TIF4631 gene.
V. VERGE, M. VONLANTHEN, J.-M. MASSON, H. TRACHSEL, and M. ALTMANN (2004)
RNA 10, 277-286
   Abstract »    Full Text »    PDF »
The roles of individual eukaryotic translation initiation factors in ribosomal scanning and initiation codon selection.
T. V. Pestova and V. G. Kolupaeva (2002)
Genes & Dev. 16, 2906-2922
   Abstract »    Full Text »    PDF »
Characterization of Mammalian eIF2A and Identification of the Yeast Homolog.
W. L. Zoll, L. E. Horton, A. A. Komar, J. O. Hensold, and W. C. Merrick (2002)
J. Biol. Chem. 277, 37079-37087
   Abstract »    Full Text »    PDF »
Functions of Eukaryotic Factors in Initiation of Translation.
T.V. PESTOVA and C.U.T. HELLEN (2001)
Cold Spring Harb Symp Quant Biol 66, 389-396
   Abstract »    PDF »
The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA.
J. Anderson, L. Phan, R. Cuesta, B. A. Carlson, M. Pak, K. Asano, G. R. Björk, M. Tamame, and A. G. Hinnebusch (1998)
Genes & Dev. 12, 3650-3662
   Abstract »    Full Text »
Initiation of Protein Synthesis in Mammalian Cells with Codons Other Than AUG and Amino Acids Other Than Methionine.
H. J. Drabkin and U. L. RajBhandary (1998)
Mol. Cell. Biol. 18, 5140-5147
   Abstract »    Full Text »
Promotion of Met-tRNAiMet Binding to Ribosomes by yIF2, a Bacterial IF2 Homolog in Yeast.
S. K. Choi, J. H. Lee, W. L. Zoll, W. C. Merrick, and T. E. Dever (1998)
Science 280, 1757-1760
   Abstract »    Full Text »
GTP hydrolysis controls stringent selection of the AUG start codon during translation initiation in Saccharomyces cerevisiae.
H.-k. Huang, H. Yoon, E. M. Hannig, and T. F. Donahue (1997)
Genes & Dev. 11, 2396-2413
   Abstract »    Full Text »    PDF »
Translational Regulation of Yeast GCN4. A WINDOW ON FACTORS THAT CONTROL INITIATOR-tRNA BINDING TO THE RIBOSOME.
A. G. Hinnebusch (1997)
J. Biol. Chem. 272, 21661-21664
   Full Text »    PDF »
Domains of initiator tRNA and initiation codon crucial for initiator tRNA selection by Escherichia coli IF3..
D Hartz, J Binkley, T Hollingsworth, and L Gold (1990)
Genes & Dev. 4, 1790-1800
   Abstract »    PDF »
From the Cover: Methionine-independent initiation of translation in the capsid protein of an insect RNA virus.
J. Sasaki and N. Nakashima (2000)
PNAS 97, 1512-1515
   Abstract »    Full Text »    PDF »
More surprises in translation: Initiation without the initiator tRNA.
U. L. RajBhandary (2000)
PNAS 97, 1325-1327
   Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)