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Science 23 September 1988:
Vol. 241. no. 4873, pp. 1667 - 1669
DOI: 10.1126/science.2843986
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Articles

Science, Vol 241, Issue 4873, 1667-1669
Copyright © 1988 by American Association for the Advancement of Science

articles

A defective HSV-1 vector expresses Escherichia coli beta-galactosidase in cultured peripheral neurons

AI Geller and XO Breakefield

Department of Neurology, Massachusetts General Hospital, Boston 02114.

A defective herpes simplex virus 1 (HSV-1) vector, pHSVlac, has been developed that contains a transcription unit that places the Escherichia coli lacZ gene under the control of the HSV-1 immediate early 4/5 promoter. The vector pHSVlac was propagated with the HSV-1 temperature-sensitive mutant ts K as helper virus. Infection of neurons from rat superior cervical ganglia and dorsal root ganglia in primary culture resulted in stable expression of high levels of beta-galactosidase without cell death. These HSV-1 vectors should be useful for introducing genes into postmitotic cells, such as neurons, in vitro and in vivo.


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