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Science 2 September 1988:
Vol. 241. no. 4870, pp. 1210 - 1213
DOI: 10.1126/science.3413485
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Articles

Science, Vol 241, Issue 4870, 1210-1213
Copyright © 1988 by American Association for the Advancement of Science

articles

Transcription factor OTF-1 is functionally identical to the DNA replication factor NF-III

EA O'Neill, C Fletcher, CR Burrow, N Heintz, RG Roeder, and TJ Kelly

Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

Octamer transcription factor-1 (OTF-1) and nuclear factor III (NF-III) are sequence-specific DNA binding proteins that activate transcription and DNA replication, respectively. It is shown here that OTF-1 is physically and biologically indistinguishable from NF-III. This conclusion is based on the following observations. First, the two proteins have identical mobilities by SDS-polyacrylamide gel electrophoresis. Second, OTF-1 binds to the adenovirus origin of DNA replication at the same site and with the same affinity as NF-III. Third, OTF-1 can substitute for NF-III in activating the initiation of adenovirus DNA replication in vitro. Fourth, the ability of OTF-1 to stimulate viral DNA replication is dependent on the presence of an intact NF-III binding site within the origin of replication. Fifth, NF-III can substitute for OTF-1 in activating in vitro transcription from the human histone H2b promoter. These data suggest the possibility that NF-III/OTF-1 is a protein that functions in both cellular DNA replication and transcription.


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