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Science 29 July 1988:
Vol. 241. no. 4865, pp. 589 - 591
DOI: 10.1126/science.3041591
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Articles

Science, Vol 241, Issue 4865, 589-591
Copyright © 1988 by American Association for the Advancement of Science

articles

Intervacuole exchange in the yeast zygote: a new pathway in organelle communication

LS Weisman and W Wickner

Molecular Biology Institute, University of California, Los Angeles 90024.

A new pathway of vesicle traffic between organelles has been identified. The vacuoles (lysosomes) of Saccharomyces cerevisiae zygotes rapidly exchange their contents at a specific point in the cell cycle. With the use of fluorescence microscopy, "tracks" were observed that connect the original parental vacuoles to the newly forming bud vacuoles. These observations suggest that vacuole-derived vesicles rapidly move along the tracks in both directions, equilibrating vacuole contents. This rapid vesicle movement may be responsible for vacuole formation in newly developing cells.


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Fusion of Docked Membranes Requires the Armadillo Repeat Protein Vac8p.
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