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Science 29 July 1988: Vol. 241. no. 4865, pp. 585 - 589 DOI: 10.1126/science.2969618
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Articles
Science, Vol 241, Issue 4865, 585-589
Copyright © 1988 by American Association for the Advancement of Science
cDNA expression cloning of the IL-1 receptor, a member of the immunoglobulin superfamily
JE Sims,
CJ March,
D Cosman,
MB Widmer,
HR MacDonald,
CJ McMahan,
CE Grubin,
JM Wignall,
JL Jackson,
SM Call,
and
al. et
Immunex Corporation, Seattle, WA 98101.
Interleukin-1 alpha and -1 beta (IL-1 alpha and IL-1 beta) are cytokines that participate in the regulation of immune responses, inflammatory reactions, and hematopoiesis. A direct expression strategy was used to clone the receptor for IL-1 from mouse T cells. The product of the cloned complementary DNA binds both IL-1 alpha and IL-1 beta in a manner indistinguishable from that of the native T cell IL-1 receptor. The extracellular, IL-1 binding portion of the receptor is 319 amino acids in length and is composed of three immunoglobulin-like domains. The cytoplasmic portion of the receptor is 217 amino acids long.
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