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Science 1 July 1988:
Vol. 241. no. 4861, pp. 81 - 84
DOI: 10.1126/science.3164526
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Articles

Science, Vol 241, Issue 4861, 81-84
Copyright © 1988 by American Association for the Advancement of Science

articles

Estrogen binding, receptor mRNA, and biologic response in osteoblast-like osteosarcoma cells

BS Komm, CM Terpening, DJ Benz, KA Graeme, A Gallegos, M Korc, GL Greene, BW O'Malley, and MR Haussler

Department of Biochemistry, University of Arizona College of Medicine, Tucson 85724.

High specific activity estradiol labeled with iodine-125 was used to detect approximately 200 saturable, high-affinity (dissociation constant approximately equal to 1.0 nM) nuclear binding sites in rat (ROS 17/2.8) and human (HOS TE85) clonal osteoblast-like osteosarcoma cells. Of the steroids tested, only testosterone exhibited significant cross-reactivity with estrogen binding. RNA blot analysis with a complementary DNA probe to the human estrogen receptor revealed putative receptor transcripts of 6 to 6.2 kilobases in both rat and human osteosarcoma cells. Type I procollagen and transforming growth factor-beta messenger RNA levels were enhanced in cultured human osteoblast-like cells treated with 1 nM estradiol. Thus, estrogen can act directly on osteoblasts by a receptor-mediated mechanism and thereby modulate the extracellular matrix and other proteins involved in the maintenance of skeletal mineralization and remodeling.


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