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Science 24 June 1988:
Vol. 240. no. 4860, pp. 1780 - 1782
DOI: 10.1126/science.3381102
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Articles

Science, Vol 240, Issue 4860, 1780-1782
Copyright © 1988 by American Association for the Advancement of Science

articles

Restricted lateral diffusion of PH-20, a PI-anchored sperm membrane protein

BM Phelps, P Primakoff, DE Koppel, MG Low, and DG Myles

Department of Physiology, University of Connecticut Health Center, Farmington 06032.

The rate of lateral diffusion of integral membrane proteins is constrained in cells, but the constraining factors for most membrane proteins have not been defined. PH-20, a sperm surface protein involved in sperm-egg adhesion, was shown to be anchored in the plasma membrane by attachment to the lipid phosphatidylinositol and to have a diffusion rate that is highly restricted on testicular sperm, being more than a thousand times slower than lipid diffusion. These results support the hypothesis that lateral mobility of a membrane protein can be regulated exclusively by interactions of its ectodomain.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Anti-Hyaluronidase Oligosaccharide Derived from Chondroitin Sulfate A Effectively Reduces Polyspermy During In Vitro Fertilization of Porcine Oocytes.
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SAMP14, a Novel, Acrosomal Membrane-associated, Glycosylphosphatidylinositol-anchored Member of the Ly-6/Urokinase-type Plasminogen Activator Receptor Superfamily with a Role in Sperm-Egg Interaction.
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Mouse Epididymal Spam1 (PH-20) Is Released in the Luminal Fluid With its Lipid Anchor.
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Mouse Sperm Lacking Cell Surface Hyaluronidase PH-20 Can Pass through the Layer of Cumulus Cells and Fertilize the Egg.
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SAMP32, a Testis-Specific, Isoantigenic Sperm Acrosomal Membrane-Associated Protein.
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Posttranslational Processing of PH-20 During Epididymal Sperm Maturation in the Horse.
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Characterization of an 80-Kilodalton Bull Sperm Protein Identified as PH-20.
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Candidate tumor suppressor HYAL2 is a glycosylphosphatidylinositol (GPI)-anchored cell-surface receptor for jaagsiekte sheep retrovirus, the envelope protein of which mediates oncogenic transformation.
S. K. Rai, F.-M. Duh, V. Vigdorovich, A. Danilkovitch-Miagkova, M. I. Lerman, and A. D. Miller (2001)
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Rat Sperm 2B1 Glycoprotein (PH20) Contains a C-Terminal Sequence Motif for Attachment of a Glycosyl Phosphatidylinositol Anchor. Effects of Endoproteolytic Cleavage on Hyaluronidase Activity.
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Biol Reprod 62, 1667-1676
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Regionalized Lipid Diffusion in the Plasma Membrane of Mammalian Spermatozoa.
C. A. Wolfe, P. S. James, A. R. Mackie, S. Ladha, and R. Jones (1998)
Biol Reprod 59, 1506-1514
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