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Science 10 June 1988:
Vol. 240. no. 4858, pp. 1544 - 1546
DOI: 10.1126/science.2897717
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Articles

Science, Vol 240, Issue 4858, 1544-1546
Copyright © 1988 by American Association for the Advancement of Science

articles

Inhibition of cellular proliferation by antisense oligodeoxynucleotides to PCNA cyclin

D Jaskulski, JK deRiel, WE Mercer, B Calabretta, and R Baserga

Department of Pathology, Temple University Medical School, Philadelphia, PA 19140.

The proliferating cell nuclear antigen (PCNA or cyclin) is a nuclear protein recently identified as a cofactor of DNA polymerase delta. When exponentially growing Balb/c3T3 cells are exposed to antisense oligodeoxynucleotides to PCNA, both DNA synthesis and mitosis are completely suppressed. A corresponding sense oligodeoxynucleotide has no inhibitory effects. These experiments indicate that PCNA (cyclin) is important in cellular DNA synthesis and in cell cycle progression.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Stathmin-deficient mice develop fibrosis and show delayed recovery from ischemic-reperfusion injury.
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Antagonistic regulation of the Drosophila PCNA gene promoter by DREF and Cut..
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Genes Cells 11, 499-512
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A maternal cafeteria diet during gestation and lactation promotes adiposity and impairs skeletal muscle development and metabolism in rat offspring at weaning.
S. A Bayol, B. H Simbi, and N. C Stickland (2005)
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Molecular markers for the assessment of postnatal male germ cell development in the mouse.
S. Jarvis, D. J. Elliott, D. Morgan, R. Winston, and C. Readhead (2005)
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Characterization of dRFX2, a novel RFX family protein in Drosophila.
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Induction of p53-dependent Activation of the Human Proliferating Cell Nuclear Antigen Gene in Chromatin by Ionizing Radiation.
B. Shan, J. Xu, Y. Zhuo, C. A. Morris, and G. F. Morris (2003)
J. Biol. Chem. 278, 44009-44017
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Ribozyme to Proliferating Cell Nuclear Antigen to Treat Proliferative Vitreoretinopathy.
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Spatial repression of PCNA by p53 during kidney development.
Z. Saifudeen, J. Marks, H. Du, and S. S. El-Dahr (2002)
Am J Physiol Renal Physiol 283, F727-F733
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Synergism Between FSH and Activin in the Regulation of Proliferating Cell Nuclear Antigen (PCNA) and Cyclin D2 Expression in Rat Granulosa Cells.
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Expression of a Novel RNA-Splicing Factor, RA301/Tra2{beta}, in Vascular Lesions and Its Role in Smooth Muscle Cell Proliferation.
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Dynamics of DNA Replication Factories in Living Cells.
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J. Cell Biol. 149, 271-280
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Factors Underlying the Cell Growth-related Bystander Responses to {{alpha}} Particles.
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Strong Epidermal Growth Factor Receptor Expression But Not HER2/neu Expression Correlates with Cell Proliferation in Anal Canal Carcinomas.
Y. Z. Hui, A. E. Noffsinger, M. A. Miller, P. Hurtubise, and C. M. Fenoglio-Preiser (1999)
International Journal of Surgical Pathology 7, 193-203
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Chimeric DNA-RNA Hammerhead Ribozyme to Proliferating Cell Nuclear Antigen Reduces Stent-Induced Stenosis in a Porcine Coronary Model.
A. Frimerman, P. J. Welch, X. Jin, N. Eigler, S. Yei, J. Forrester, H. Honda, R. Makkar, J. Barber, and F. Litvack (1999)
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p53-Mediated Regulation of Proliferating Cell Nuclear Antigen Expression in Cells Exposed to Ionizing Radiation.
J. Xu and G. F. Morris (1999)
Mol. Cell. Biol. 19, 12-20
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In Vitro Culture of Hamster Ovarian Primary Interstitial Cells: Effect of Serum.
J. R. Schwartz and S. K. Roy (1998)
Biol Reprod 59, 1187-1194
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Isolation and Analysis of a T Cell Clone Variant Exhibiting Constitutively Phosphorylated Ser133 cAMP Response Element-Binding Protein.
S. M. Belkowski, C. S. Rubin, and M. B. Prystowsky (1998)
J. Immunol. 161, 659-665
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Regulation of the Human Proliferating Cell Nuclear Antigen Promoter by the Adenovirus E1A-Associated Protein p107.
B. H. Lee, M. Liu, and M. B. Mathews (1998)
J. Virol. 72, 1138-1145
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von Willebrand Factor Does Not Influence Atherogenesis in Arteries Subjected to Altered Shear Stress.
T. C. Nichols, D. A. Bellinger, R. L. Reddick, G. G. Koch, J. L. Sigman, G. Erickson, T. du Laney, T. Johnson, M. S. Read, and T. R. Griggs (1998)
Arterioscler. Thromb. Vasc. Biol. 18, 323-330
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Identification of CFDD (Common Regulatory Factor for DNA Replication and DREF Genes) and Role of Its Binding Site in Regulation of the Proliferating Cell Nuclear Antigen Gene Promoter.
Y. Hayashi, F. Hirose, Y. Nishimoto, M. Shiraki, M. Yamagishi, A. Matsukage, and M. Yamaguchi (1997)
J. Biol. Chem. 272, 22848-22858
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Processing of Chimeric Antisense Oligonucleotides by Human Vascular Smooth Muscle Cells and Human Atherosclerotic Plaque : Implications for Antisense Therapy of Restenosis After Angioplasty.
J. G. Pickering, J. M. Isner, C. M. Ford, L. Weir, A. Lazarovits, E. F. Rocnik, and L. H. Chow (1996)
Circulation 93, 772-780
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Identification of an Essential Cis-Element Near the Transcription Start Site for Transcriptional Activation of the Proliferating Cell Nuclear Antigen Gene.
D. Y. Huang and M. B. Prystowsky (1996)
J. Biol. Chem. 271, 1218-1225
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Structure-Function Relationship of the Eukaryotic DNA Replication Factor, Proliferating Cell Nuclear Antigen.
K. Fukuda, H. Morioka, S. Imajou, S. Ikeda, E. Ohtsuka, and T. Tsurimoto (1995)
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Cyclin D1-mediated inhibition of repair and replicative DNA synthesis in human fibroblasts..
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Clear Cell Sarcoma of Tendons and Aponeuroses: A Clinicopathologic Study of 58 Cases With Analysis of Prognostic Factors.
E. A. Montgomery, J. M. Meis, A. G. Ramos, D. M. Frisman, and K. L. Martz (1993)
International Journal of Surgical Pathology 1, 89-99
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Demonstration of cell cycle kinetics in thyroid primary culture by immunostaining of proliferating cell nuclear antigen: differences in cyclic AMP-dependent and -independent mitogenic stimulations.
M Baptist, J. Dumont, and P. Roger (1993)
J. Cell Sci. 105, 69-80
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A c-myb antisense oligodeoxynucleotide inhibits normal human hematopoiesis in vitro.
A. Gewirtz and B Calabretta (1988)
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Regulation of Apoptosis and Cell Cycle Progression by MCL1. DIFFERENTIAL ROLE OF PROLIFERATING CELL NUCLEAR ANTIGEN.
K. Fujise, D. Zhang, J.-l. Liu, and E. T. H. Yeh (2000)
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