Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 3 June 1988:
Vol. 240. no. 4857, pp. 1339 - 1341
DOI: 10.1126/science.2897715
Prev | Table of Contents | Next

Articles

Science, Vol 240, Issue 4857, 1339-1341
Copyright © 1988 by American Association for the Advancement of Science

articles

Expression of the beta-nerve growth factor gene in hippocampal neurons

C Ayer-LeLievre, L Olson, T Ebendal, A Seiger, and H Persson

Department of Medical Chemistry, Karolinska Institutet, Stockholm, Sweden.

In situ hybridization with complementary DNA probes for nerve growth factor (NGF) was used to identify cells containing NGF messenger RNA in rat and mouse brain. The most intense labeling occurred in hippocampus, where hybridizing neurons were found in the dentate gyrus and the pyramidal cell layer. The neuronal identity of NGF mRNA-containing cells was further assessed by a loss of NGF-hybridizing mRNA in hippocampal areas where neurons had been destroyed by kainic acid or colchicine. RNA blot analysis also revealed a considerable decrease in the level of NGF mRNA in rat dentate gyrus after a lesion was produced by colchicine. This lesion also caused a decrease in the level of Thy-1 mRNA and an increase in the level of glial fibrillary acidic protein mRNA. Neuronal death was thus associated with the disappearance of NGF mRNA. These results suggest a synthesis of NGF by neurons in the brain and imply that, in hippocampus, NGF influences NGF-sensitive neurons through neuron-to-neuron interactions.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Function of Activity-Regulated Genes in the Nervous System.
S. Loebrich and E. Nedivi (2009)
Physiol Rev 89, 1079-1103
   Abstract »    Full Text »    PDF »
Immunohistochemical Distribution of NGF, BDNF, NT-3, and NT-4 in Adult Rhesus Monkey Brains.
H.-T. Zhang, L.-Y. Li, X.-L. Zou, X.-B. Song, Y.-L. Hu, Z.-T. Feng, and T. T.-H. Wang (2007)
J. Histochem. Cytochem. 55, 1-19
   Abstract »    Full Text »    PDF »
Brain-Derived Neurotrophic Factor Activation of NFAT (Nuclear Factor of Activated T-Cells)-Dependent Transcription: A Role for the Transcription Factor NFATc4 in Neurotrophin-Mediated Gene Expression.
R. D. Groth and P. G. Mermelstein (2003)
J. Neurosci. 23, 8125-8134
   Abstract »    Full Text »    PDF »
Expression of Nerve Growth Factor and trkA After Transient Focal Cerebral Ischemia in Rats • Editorial Comment.
T.-H. Lee, H. Kato, S.-T. Chen, K. Kogure, Y. Itoyama, and C. D. Kontos (1998)
Stroke 29, 1687-1697
   Abstract »    Full Text »    PDF »
Regulation of Nerve Growth Factor mRNA by Interleukin-1 in Rat Hippocampal Astrocytes Is Mediated by NFkappa B.
W. J. Friedman, S. Thakur, L. Seidman, and A. B. Rabson (1996)
J. Biol. Chem. 271, 31115-31120
   Abstract »    Full Text »    PDF »
Neurotrophins and Neuronal Plasticity.
H. Thoenen (1995)
Science 270, 593-598
   Abstract »    PDF »
Monitoring release of neurotrophic activity in the brains of awake rats.
C Humpel, E Lindqvist, S Soderstrom, A Kylberg, T Ebendal, and L Olson (1995)
Science 269, 552-554
   Abstract »    PDF »
Intraputaminal Infusion of Nerve Growth Factor to Support Adrenal Medullary Autografts in Parkinson's Disease: One-Year Follow-up of First Clinical Trial.
L. Olson, E.-O. Backlund, T. Ebendal, R. Freedman, B. Hamberger, P. Hansson, B. Hoffer, U. Lindblom, B. Meyerson, I. Stromberg, et al. (1991)
Arch Neurol 48, 373-381
   Abstract »    PDF »
Widespread expression of BDNF but not NT3 by target areas of basal forebrain cholinergic neurons.
H. Phillips, J. Hains, G. Laramee, A Rosenthal, and J. Winslow (1990)
Science 250, 290-294
   Abstract »    PDF »
Survival of adult basal forebrain cholinergic neurons after loss of target neurons.
M. Sofroniew, N. Galletly, O Isacson, and C. Svendsen (1990)
Science 247, 338-342
   Abstract »    PDF »
Limbic seizures increase neuronal production of messenger RNA for nerve growth factor.
C. Gall and P. Isackson (1989)
Science 245, 758-761
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)