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Science 3 June 1988:
Vol. 240. no. 4857, pp. 1302 - 1309
DOI: 10.1126/science.3287616

Articles

Science, Vol 240, Issue 4857, 1302-1309
Copyright © 1988 by American Association for the Advancement of Science


articles

DNA damage and oxygen radical toxicity

JA Imlay and S Linn

University of California, Berkeley.

A major portion of the toxicity of hydrogen peroxide in Escherichia coli is attributed to DNA damage mediated by a Fenton reaction that generates active forms of hydroxyl radicals from hydrogen peroxide, DNA-bound iron, and a constant source of reducing equivalents. Kinetic peculiarities of DNA damage production by hydrogen peroxide in vivo can be reproduced by including DNA in an in vitro Fenton reaction system in which iron catalyzes the univalent reduction of hydrogen peroxide by the reduced form of nicotinamide adenine dinucleotide (NADH). To minimize the toxicity of oxygen radicals, the cell utilizes scavengers of these radicals and DNA repair enzymes. On the basis of observations with the model system, it is proposed that the cell may also decrease such toxicity by diminishing available NAD(P)H and by utilizing oxygen itself to scavenge active free radicals into superoxide, which is then destroyed by superoxide dismutase.


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   Abstract »    Full Text »    PDF »
The effect of ascorbate and {alpha}-tocopherol supplementation in vitro on DNA integrity and hydrogen peroxide-induced DNA damage in human spermatozoa.
E. T. Donnelly, N. McClure, and S. E.M. Lewis (1999)
Mutagenesis 14, 505-512
   Abstract »    Full Text »    PDF »
The Activation of the Rat Copper/Zinc Superoxide Dismutase Gene by Hydrogen Peroxide through the Hydrogen Peroxide-responsive Element and by Paraquat and Heat Shock through the Same Heat Shock Element.
H. Y. Yoo, M. S. Chang, and H. M. Rho (1999)
J. Biol. Chem. 274, 23887-23892
   Abstract »    Full Text »    PDF »
Role of Superoxide Dismutase Activity in the Physiology of Porphyromonas gingivalis.
M. C. Lynch and H. K. Kuramitsu (1999)
Infect. Immun. 67, 3367-3375
   Abstract »    Full Text »    PDF »
Characterization of the Major Superoxide Dismutase of Staphylococcus aureus and Its Role in Starvation Survival, Stress Resistance, and Pathogenicity.
M. O. Clements, S. P. Watson, and S. J. Foster (1999)
J. Bacteriol. 181, 3898-3903
   Abstract »    Full Text »
SodA and Manganese Are Essential for Resistance to Oxidative Stress in Growing and Sporulating Cells of Bacillus subtilis.
T. Inaoka, Y. Matsumura, and T. Tsuchido (1999)
J. Bacteriol. 181, 1939-1943
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1,25-Dihydroxyvitamin D3 Enhances the Susceptibility of Breast Cancer Cells to Doxorubicin-induced Oxidative Damage.
A. Ravid, D. Rocker, A. Machlenkin, C. Rotem, A. Hochman, G. Kessler-Icekson, U. A. Liberman, and R. Koren (1999)
Cancer Res. 59, 862-867
   Abstract »    Full Text »    PDF »
Escherichia coli MutM Suppresses Illegitimate Recombination Induced by Oxidative Stress.
M. Onda, K. Hanada, H. Kawachi, and H. Ikeda (1999)
Genetics 151, 439-446
   Abstract »    Full Text »
Sequence-specific DNA Cleavage by Fe2+-mediated Fenton Reactions Has Possible Biological Implications.
E. S. Henle, Z. Han, N. Tang, P. Rai, Y. Luo, and S. Linn (1999)
J. Biol. Chem. 274, 962-971
   Abstract »    Full Text »    PDF »
Detection of the `4977 bp' mitochondrial DNA deletion in human atherosclerotic lesions.
M. Bogliolo, A. Izzotti, S. De Flora, C. Carli, A. Abbondandolo, and P. Degan (1999)
Mutagenesis 14, 77-82
   Abstract »    Full Text »    PDF »
Characterization of a Peroxide-Resistant Mutant of the Anaerobic Bacterium Bacteroides fragilis.
E. R. Rocha and C. J. Smith (1998)
J. Bacteriol. 180, 5906-5912
   Abstract »    Full Text »



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