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Science 3 June 1988: Vol. 240. no. 4857, pp. 1302 - 1309 DOI: 10.1126/science.3287616
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Articles
Science, Vol 240, Issue 4857, 1302-1309
Copyright © 1988 by American Association for the Advancement of Science
DNA damage and oxygen radical toxicity
JA Imlay
and
S Linn
University of California, Berkeley.
A major portion of the toxicity of hydrogen peroxide in Escherichia coli is attributed to DNA damage mediated by a Fenton reaction that generates active forms of hydroxyl radicals from hydrogen peroxide, DNA-bound iron, and a constant source of reducing equivalents. Kinetic peculiarities of DNA damage production by hydrogen peroxide in vivo can be reproduced by including DNA in an in vitro Fenton reaction system in which iron catalyzes the univalent reduction of hydrogen peroxide by the reduced form of nicotinamide adenine dinucleotide (NADH). To minimize the toxicity of oxygen radicals, the cell utilizes scavengers of these radicals and DNA repair enzymes. On the basis of observations with the model system, it is proposed that the cell may also decrease such toxicity by diminishing available NAD(P)H and by utilizing oxygen itself to scavenge active free radicals into superoxide, which is then destroyed by superoxide dismutase.
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- W. G. Dos Santos, I. Pacheco, M.-Y. Liu, M. Teixeira, A. V. Xavier, and J. LeGall (2000)
J. Bacteriol.
182, 796-804
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- Contribution of NADH Oxidase to Aerobic Metabolism of Streptococcus pyogenes.
- C. M. Gibson, T. C. Mallett, A. Claiborne, and M. G. Caparon (2000)
J. Bacteriol.
182, 448-455
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- Glutathione and hypotaurine in vitro: effects on human sperm motility, DNA integrity and production of reactive oxygen species.
- E. T. Donnelly, N. Mcclure, and S. E.M. Lewis (2000)
Mutagenesis
15, 61-68
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- Role of Iron and Superoxide for Generation of Hydroxyl Radical, Oxidative DNA Lesions, and Mutagenesis in Escherichia coli.
- T. Nunoshiba, F. Obata, A. C. Boss, S. Oikawa, T. Mori, S. Kawanishi, and K. Yamamoto (1999)
J. Biol. Chem.
274, 34832-34837
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- The roles of free radicals in amyotrophic lateral sclerosis: reactive oxygen species and elevated oxidation of protein, DNA, and membrane phospholipids.
- D. LIU, J. WEN, J. LIU, and L. LI (1999)
FASEB J
13, 2318-2328
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- Oxidative Stress and Iron Are Implicated in Fragmenting Vacuoles of Saccharomyces cerevisiae Lacking Cu,Zn-Superoxide Dismutase.
- L. B. Corson, J. Folmer, J. J. Strain, V. C. Culotta, and D. W. Cleveland (1999)
J. Biol. Chem.
274, 27590-27596
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- The effect of ascorbate and {alpha}-tocopherol supplementation in vitro on DNA integrity and hydrogen peroxide-induced DNA damage in human spermatozoa.
- E. T. Donnelly, N. McClure, and S. E.M. Lewis (1999)
Mutagenesis
14, 505-512
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- The Activation of the Rat Copper/Zinc Superoxide Dismutase Gene by Hydrogen Peroxide through the Hydrogen Peroxide-responsive Element and by Paraquat and Heat Shock through the Same Heat Shock Element.
- H. Y. Yoo, M. S. Chang, and H. M. Rho (1999)
J. Biol. Chem.
274, 23887-23892
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- Role of Superoxide Dismutase Activity in the Physiology of Porphyromonas gingivalis.
- M. C. Lynch and H. K. Kuramitsu (1999)
Infect. Immun.
67, 3367-3375
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- Characterization of the Major Superoxide Dismutase of Staphylococcus aureus and Its Role in Starvation Survival, Stress Resistance, and Pathogenicity.
- M. O. Clements, S. P. Watson, and S. J. Foster (1999)
J. Bacteriol.
181, 3898-3903
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- SodA and Manganese Are Essential for Resistance to Oxidative Stress in Growing and Sporulating Cells of Bacillus subtilis.
- T. Inaoka, Y. Matsumura, and T. Tsuchido (1999)
J. Bacteriol.
181, 1939-1943
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- 1,25-Dihydroxyvitamin D3 Enhances the Susceptibility of Breast Cancer Cells to Doxorubicin-induced Oxidative Damage.
- A. Ravid, D. Rocker, A. Machlenkin, C. Rotem, A. Hochman, G. Kessler-Icekson, U. A. Liberman, and R. Koren (1999)
Cancer Res.
59, 862-867
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- Escherichia coli MutM Suppresses Illegitimate Recombination Induced by Oxidative Stress.
- M. Onda, K. Hanada, H. Kawachi, and H. Ikeda (1999)
Genetics
151, 439-446
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- Sequence-specific DNA Cleavage by Fe2+-mediated Fenton Reactions Has Possible Biological Implications.
- E. S. Henle, Z. Han, N. Tang, P. Rai, Y. Luo, and S. Linn (1999)
J. Biol. Chem.
274, 962-971
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- Detection of the `4977 bp' mitochondrial DNA deletion in human atherosclerotic lesions.
- M. Bogliolo, A. Izzotti, S. De Flora, C. Carli, A. Abbondandolo, and P. Degan (1999)
Mutagenesis
14, 77-82
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- Characterization of a Peroxide-Resistant Mutant of the Anaerobic Bacterium Bacteroides fragilis.
- E. R. Rocha and C. J. Smith (1998)
J. Bacteriol.
180, 5906-5912
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