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Science 20 May 1988:
Vol. 240. no. 4855, pp. 1041 - 1043
DOI: 10.1126/science.3285471
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Articles

Science, Vol 240, Issue 4855, 1041-1043
Copyright © 1988 by American Association for the Advancement of Science

articles

Escherichia coli secretion of an active chimeric antibody fragment

M Better, CP Chang, RR Robinson, and AH Horwitz

International Genetic Engineering Inc. (INGENE), Santa Monica, CA 90404.

A chimeric mouse-human Fab protein that binds specifically to the human carcinoma cell line C3347 has been expressed and secreted from Escherichia coli. This molecule, which contains functionally assembled kappa and Fd proteins, binds as effectively to sites on the surface of C3347 cells as Fab fragments prepared proteolytically from whole chimeric or mouse antibody. The production in Escherichia coli of foreign heterodimeric protein reagents, such as Fab, should prove useful in the management of human disease.


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