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Science 20 May 1988:
Vol. 240. no. 4855, pp. 1010 - 1016
DOI: 10.1126/science.3130660

Articles

Science, Vol 240, Issue 4855, 1010-1016
Copyright © 1988 by American Association for the Advancement of Science


articles

Fos-associated protein p39 is the product of the jun proto-oncogene

FJ Rauscher 3rd, DR Cohen, T Curran, TJ Bos, PK Vogt, D Bohmann, R Tjian, and BR Franza Jr

Roche Institute for Molecular Biology, Nutley, NJ 07110.

The Fos protein complex and several Fos-related antigens (FRA) bind specifically to a sequence element referred to as the HeLa cell activator protein 1 (AP-1) binding site. A combination of structural and immunological comparisons has identified the Fos-associated protein (p39) as the protein product of the jun proto-oncogene (c-Jun). The p39/Jun protein is one of the major polypeptides identified in AP-1 oligonucleotide affinity chromatography extracts of cellular proteins. These preparations of AP-1 also contain Fos and several FRA's. Some of these proteins bind to the AP-1 site directly whereas others, like Fos, appear to bind indirectly via protein-protein interactions. Cell-surface stimulation results in an increase in c-fos and c-jun products. Thus, the products of two protooncogenes (and several related proteins), induced by extracellular stimuli, form a complex that associates with transcriptional control elements containing AP-1 sites, thereby potentially mediating the long-term responses to signals that regulate growth control and development.


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Leucine repeats and an adjacent DNA binding domain mediate the formation of functional cFos-cJun heterodimers.
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Parallel association of Fos and Jun leucine zippers juxtaposes DNA binding domains.
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Multiple sequence elements in the c-fos promoter mediate induction by cAMP..
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Evidence that the leucine zipper is a coiled coil.
E. O'Shea, R Rutkowski, and P. Kim (1989)
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The c-fos transcript is targeted for rapid decay by two distinct mRNA degradation pathways..
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T Williams, A Admon, B Luscher, and R Tjian (1988)
Genes & Dev. 2, 1557-1569
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Fos and Jun bind cooperatively to the AP-1 site: reconstitution in vitro..
F J Rauscher, P J Voulalas, B R Franza, and T Curran (1988)
Genes & Dev. 2, 1687-1699
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A family of immunologically related transcription factors that includes multiple forms of ATF and AP-1..
T W Hai, F Liu, E A Allegretto, M Karin, and M R Green (1988)
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The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins.
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Science. ISSN 0036-8075 (print), 1095-9203 (online)