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Science 13 May 1988:
Vol. 240. no. 4854, pp. 911 - 913
DOI: 10.1126/science.2834825
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Articles

Science, Vol 240, Issue 4854, 911-913
Copyright © 1988 by American Association for the Advancement of Science

articles

Calpain II involvement in mitosis

JE Schollmeyer

U.S. Department of Agriculture, Roman L. Hruska Meat Animal Research Center, Clay Center, NE 68933.

Mitotic spindle disassembly requires major structural alterations in the associated cytoskeletal proteins and mitosis is known to be associated with Ca2+-sequestering phenomena and calcium transients. To examine the possible involvement of a ubiquitous Ca2+-activated protease, calpain II, in the mitotic process, synchronized PtK1 cells were monitored by immunofluorescence for the relocation of calpain II. The plasma membrane was the predominant location of calpain II in interphase. However, as mitosis progressed, calpain II relocated to (i) an association with mitotic chromosomes, (ii) a perinuclear location in anaphase, and (iii) a mid-body location in telophase. Microinjection of calpain II near the nucleus of a PtK1 cell promoted the onset of metaphase. Injection of calpain II at late metaphase promoted a precocious disassembly of the mitotic spindle and the onset of anaphase. These data suggest that calpain II is involved in mitosis.


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