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Science 6 May 1988:
Vol. 240. no. 4853, pp. 800 - 802
DOI: 10.1126/science.2452484
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Articles

Science, Vol 240, Issue 4853, 800-802
Copyright © 1988 by American Association for the Advancement of Science

articles

Proteosome-lipopeptide vaccines: enhancement of immunogenicity for malaria CS peptides

GH Lowell, WR Ballou, LF Smith, RA Wirtz, WD Zollinger, and WT Hockmeyer

Department of Bacterial Diseases, Walter Reed Army Institute of Research, Washington, DC 20307-5100.

Proteosomes are hydrophobic, membranous, multimolecular preparations of meningococcal outer membrane proteins that are also B cell mitogens. These characteristics suggested that proteosomes may serve as carrier proteins and adjuvants to enhance peptide immunogenicity. Although high titers of malaria circumsporozoite (CS) antibodies protect against malaria, vaccines thus far tested in humans have been insufficiently immunogenic to be clinically useful. Here it is shown that synthetic CS peptides hydrophobically complexed to proteosomes by way of lauroyl-cysteine become highly immunogenic in mice without other adjuvants. The high titers of antibodies produced and the safety of proteosomes in humans suggest that this novel system is widely applicable for the development of peptide vaccines to protect against many diseases.


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