Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 6 May 1988:
Vol. 240. no. 4853, pp. 793 - 796
DOI: 10.1126/science.2452483
Prev | Table of Contents | Next

Articles

Science, Vol 240, Issue 4853, 793-796
Copyright © 1988 by American Association for the Advancement of Science

articles

Changing the identity of a tRNA by introducing a G-U wobble pair near the 3' acceptor end

WH McClain and K Foss

Department of Bacteriology, University of Wisconsin, Madison 53706.

Although the genetic code for protein was established in the 1960's, the basis for amino acid identity of transfer RNA (tRNA) has remained unknown. To investigate the identity of a tRNA, the nucleotides at three computer-identified positions in tRNAPhe (phenylalanine tRNA) were replaced with the corresponding nucleotides from tRNAAla (alanine tRNA). The identity of the resulting tRNA, when examined as an amber suppressor in Escherichia coli, was that of tRNAAla.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Unique protein architecture of alanyl-tRNA synthetase for aminoacylation, editing, and dimerization.
M. Naganuma, S.-i. Sekine, R. Fukunaga, and S. Yokoyama (2009)
PNAS 106, 8489-8494
   Abstract »    Full Text »    PDF »
The electrostatic characteristics of G{middle dot}U wobble base pairs.
D. Xu, T. Landon, N. L. Greenbaum, and M. O. Fenley (2007)
Nucleic Acids Res. 35, 3836-3847
   Abstract »    Full Text »    PDF »
Misacylation of Yeast Amber Suppressor tRNATyr by E. coli Lysyl-tRNA Synthetase and Its Effective Repression by Genetic Engineering of the tRNA Sequence..
J.-i. Fukunaga, T. Yokogawa, S. Ohno, and K. Nishikawa (2006)
J. Biochem. 139, 689-696
   Abstract »    Full Text »    PDF »
Surprising contribution to aminoacylation and translation of non-Watson-Crick pairs in tRNA.
W. H. McClain (2006)
PNAS 103, 4570-4575
   Abstract »    Full Text »    PDF »
Use of RNase P for Efficient Preparation of Yeast tRNATyr Transcript and Its Mutants.
J.-i. Fukunaga, M. Gouda, K. Umeda, S. Ohno, T. Yokogawa, and K. Nishikawa (2006)
J. Biochem. 139, 123-127
   Abstract »    Full Text »    PDF »
A Mutation in the Anticodon of a Single tRNAala Is Sufficient to Confer Auxin Resistance in Arabidopsis.
J. Perry, X. Dai, and Y. Zhao (2005)
Plant Physiology 139, 1284-1290
   Abstract »    Full Text »    PDF »
Effects of nucleotide substitution and modification on the stability and structure of helix 69 from 28S rRNA.
M. SUMITA, J.-P. DESAULNIERS, Y.-C. CHANG, H. M.-P. CHUI, L. CLOS II, and C. S. CHOW (2005)
RNA 11, 1420-1429
   Abstract »    Full Text »    PDF »
Independent binding sites of small protein B onto transfer-messenger RNA during trans-translation.
L. Metzinger, M. Hallier, and B. Felden (2005)
Nucleic Acids Res. 33, 2384-2394
   Abstract »    Full Text »    PDF »
Structure-function analysis of tRNAGln in an Escherichia coli knockout strain.
W. H. MCCLAIN, K. GABRIEL, D. LEE, and S. OTTEN (2004)
RNA 10, 795-804
   Abstract »    Full Text »    PDF »
Structure-specific tRNA Determinants for Editing a Mischarged Amino Acid.
K. Beebe, E. Merriman, and P. Schimmel (2003)
J. Biol. Chem. 278, 45056-45061
   Abstract »    Full Text »    PDF »
Importance of the reverse Hoogsteen base pair 54-58 for tRNA function.
E. I. Zagryadskaya, F. R. Doyon, and S. V. Steinberg (2003)
Nucleic Acids Res. 31, 3946-3953
   Abstract »    Full Text »    PDF »
RNA recognition by designed peptide fusion creates "artificial" tRNA synthetase.
M. Frugier, R. Giege, and P. Schimmel (2003)
PNAS 100, 7471-7475
   Abstract »    Full Text »    PDF »
SmpB functions in various steps of trans-translation.
K. Hanawa-Suetsugu, M. Takagi, H. Inokuchi, H. Himeno, and A. Muto (2002)
Nucleic Acids Res. 30, 1620-1629
   Abstract »    Full Text »    PDF »
Dual Mode Recognition of Two Isoacceptor tRNAs by Mammalian Mitochondrial Seryl-tRNA Synthetase.
N. Shimada, T. Suzuki, and K. Watanabe (2001)
J. Biol. Chem. 276, 46770-46778
   Abstract »    Full Text »    PDF »
Importance of the conserved nucleotides around the tRNA-like structure of Escherichia coli transfer-messenger RNA for protein tagging.
K. Hanawa-Suetsugu, V. Bordeau, H. Himeno, A. Muto, and B. Felden (2001)
Nucleic Acids Res. 29, 4663-4673
   Abstract »    Full Text »    PDF »
Quality Control of the Elongation Step of Protein Synthesis by tmRNP.
J. Wower, I. K. Wower, B. Kraal, and C. W. Zwieb (2001)
J. Nutr. 131, 2978S-2982
   Abstract »    Full Text »    PDF »
Synthesis and crystal structure of an octamer RNA r(guguuuac)/r(guaggcac) with G{middle dot}G/U{middle dot}U tandem wobble base pairs: comparison with other tandem G{middle dot}U pairs.
J. Deng and M. Sundaralingam (2000)
Nucleic Acids Res. 28, 4376-4381
   Abstract »    Full Text »    PDF »
Importance of discriminator base stacking interactions: molecular dynamics analysis of A73 microhelixAla variants.
M. C. Nagan, P. Beuning, K. Musier-Forsyth, and C. J. Cramer (2000)
Nucleic Acids Res. 28, 2527-2534
   Abstract »    Full Text »    PDF »
Identification of Discriminator Base Atomic Groups That Modulate the Alanine Aminoacylation Reaction.
A. E. Fischer, P. J. Beuning, and K. Musier-Forsyth (1999)
J. Biol. Chem. 274, 37093-37096
   Abstract »    Full Text »    PDF »
Assembly of a catalytic unit for RNA microhelix aminoacylation using nonspecific RNA binding domains.
J. W. Chihade and P. Schimmel (1999)
PNAS 96, 12316-12321
   Abstract »    Full Text »    PDF »
Correlation of deformability at a tRNA recognition site and aminoacylation specificity.
K.-Y. Chang, G. Varani, S. Bhattacharya, H. Choi, and W. H. McClain (1999)
PNAS 96, 11764-11769
   Abstract »    Full Text »    PDF »
Analysis of the Role of trans-Translation in the Requirement of tmRNA for lambda immP22 Growth in Escherichia coli.
J. Withey and D. Friedman (1999)
J. Bacteriol. 181, 2148-2157
   Abstract »    Full Text »
Diverse RNA substrates for aminoacylation: Clues to origins?.
P. Schimmel and R. Alexander (1998)
PNAS 95, 10351-10353
   Full Text »    PDF »
Sequences Outside Recognition Sets Are Not Neutral for tRNA Aminoacylation. EVIDENCE FOR NONPERMISSIVE COMBINATIONS OF NUCLEOTIDES IN THE ACCEPTOR STEM OF YEAST tRNAPhe.
M. Frugier, M. Helm, B. Felden, R. Giege, and C. Florentz (1998)
J. Biol. Chem. 273, 11605-11610
   Abstract »    Full Text »    PDF »
Identification in a pseudoknot of a U·G motif essential for the regulation of the expression of ribosomal protein S15.
L. Benard, N. Mathy, M. Grunberg-Manago, B. Ehresmann, C. Ehresmann, and C. Portier (1998)
PNAS 95, 2564-2567
   Abstract »    Full Text »    PDF »
Amber suppression in Escherichia coli by unusual mitochondria-like transfer RNAs.
V. Bourdeau, S. V. Steinberg, G. Ferbeyre, R. Emond, N. Cermakian, and R. Cedergren (1998)
PNAS 95, 1375-1380
   Abstract »    Full Text »    PDF »
Subtle atomic group discrimination in the RNA minor groove.
M. Frugier and P. Schimmel (1997)
PNAS 94, 11291-11294
   Abstract »    Full Text »    PDF »
Specific atomic groups and RNA helix geometry in acceptor stem recognition by a tRNA synthetase.
P. J. Beuning, F. Yang, P. Schimmel, and K. Musier-Forsyth (1997)
PNAS 94, 10150-10154
   Abstract »    Full Text »    PDF »
The transfer RNA identity problem: a search for rules.
M. Saks, Sampson JR, and J. Abelson (1994)
Science 263, 191-197
   Abstract »    PDF »
Overlapping nucleotide determinants for specific aminoacylation of RNA microhelices.
C Francklyn, J. Shi, and P Schimmel (1992)
Science 255, 1121-1125
   Abstract »    PDF »
Class II aminoacyl transfer RNA synthetases: crystal structure of yeast aspartyl-tRNA synthetase complexed with tRNA(Asp).
M Ruff, S Krishnaswamy, M Boeglin, A Poterszman, A Mitschler, A Podjarny, B Rees, J. Thierry, and D Moras (1991)
Science 252, 1682-1689
   Abstract »    PDF »
Identity elements for specific aminoacylation of yeast tRNA(Asp) by cognate aspartyl-tRNA synthetase.
J Putz, J. Puglisi, C Florentz, and R Giege (1991)
Science 252, 1696-1699
   Abstract »    PDF »
The anticodon contains a major element of the identity of arginine transfer RNAs.
L. Schulman and H Pelka (1989)
Science 246, 1595-1597
   Abstract »    PDF »
Structure of E. coli glutaminyl-tRNA synthetase complexed with tRNA(Gln) and ATP at 2.8 A resolution.
M. Rould, J. Perona, D Soll, and T. Steitz (1989)
Science 246, 1135-1142
   Abstract »    PDF »
Structural basis for misaminoacylation by mutant E. coli glutaminyl-tRNA synthetase enzymes.
J. Perona, R. Swanson, M. Rould, T. Steitz, and D Soll (1989)
Science 246, 1152-1154
   Abstract »    PDF »
Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase.
J. Sampson, A. DiRenzo, L. Behlen, and O. Uhlenbeck (1989)
Science 243, 1363-1366
   Abstract »    PDF »
Association of transfer RNA acceptor identity with a helical irregularity.
W. McClain, Y. Chen, K Foss, and J Schneider (1988)
Science 242, 1681-1684
   Abstract »    PDF »
Accuracy of in vivo aminoacylation requires proper balance of tRNA and aminoacyl-tRNA synthetase.
R Swanson, P Hoben, M Sumner-Smith, H Uemura, L Watson, and D Soll (1988)
Science 242, 1548-1551
   Abstract »    PDF »
Anticodon switching changes the identity of methionine and valine transfer RNAs.
L. Schulman and H Pelka (1988)
Science 242, 765-768
   Abstract »    PDF »
Changing the acceptor identity of a transfer RNA by altering nucleotides in a "variable pocket".
W. McClain and K Foss (1988)
Science 241, 1804-1807
   Abstract »    PDF »
Recent excitement in understanding transfer RNA identity.
L. Schulman and J Abelson (1988)
Science 240, 1591-1592
   PDF »
Characterization and tRNA Recognition of Mammalian Mitochondrial Seryl-tRNA Synthetase.
T. Yokogawa, N. Shimada, N. Takeuchi, L. Benkowski, T. Suzuki, A. Omori, T. Ueda, K. Nishikawa, L. L. Spremulli, and K. Watanabe (2000)
J. Biol. Chem. 275, 19913-19920
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)