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Science 6 May 1988:
Vol. 240. no. 4853, pp. 793 - 796
DOI: 10.1126/science.2452483
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Articles

Science, Vol 240, Issue 4853, 793-796
Copyright © 1988 by American Association for the Advancement of Science

articles

Changing the identity of a tRNA by introducing a G-U wobble pair near the 3' acceptor end

WH McClain and K Foss

Department of Bacteriology, University of Wisconsin, Madison 53706.

Although the genetic code for protein was established in the 1960's, the basis for amino acid identity of transfer RNA (tRNA) has remained unknown. To investigate the identity of a tRNA, the nucleotides at three computer-identified positions in tRNAPhe (phenylalanine tRNA) were replaced with the corresponding nucleotides from tRNAAla (alanine tRNA). The identity of the resulting tRNA, when examined as an amber suppressor in Escherichia coli, was that of tRNAAla.


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Recent excitement in understanding transfer RNA identity.
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