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Science 6 May 1988:
Vol. 240. no. 4853, pp. 768 - 772
DOI: 10.1126/science.3363358
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Articles

Science, Vol 240, Issue 4853, 768-772
Copyright © 1988 by American Association for the Advancement of Science

articles

Visualizing gene expression in time and space in the filamentous bacterium Streptomyces coelicolor

A Schauer, M Ranes, R Santamaria, J Guijarro, E Lawlor, C Mendez, K Chater, and R Losick

Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138.

Streptomycetes are prokaryotic microorganisms that exhibit a complex, mycelial fungus-like cycle of morphological differentiation. Development involves at least two spatially separated types of cells: the branching hyphae of the substrate mycelium, which penetrate the stratum upon which the colony feeds, and the upwardly protruding hyphae of the aerial mycelium, which undergo metamorphosis into spores. The luciferase-encoding luxA and luxB operon of the luminescent marine bacterium Vibrio harveyi was used as a promoter probe to visualize gene expression in differentiating colonies of Streptomyces coelicolor. Promoters for developmental genes of several kinds gave distinctive temporal and spatial patterns of light emission.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Integrating the Circadian Oscillator into the Life of the Cyanobacterial Cell.
S. S. Golden (2007)
Cold Spring Harb Symp Quant Biol 72, 331-338
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Heterogeneous distribution of lysine 6-aminotransferase during cephamycin C biosynthesis in Streptomyces clavuligerus demonstrated using green fluorescent protein as a reporter.
A. Khetan, W.-S. Hu, and D. H. Sherman (2000)
Microbiology 146, 1869-1880
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Green fluorescent protein as a reporter for spatial and temporal gene expression in Streptomyces coelicolor A3(2).
J. Sun, G. H. Kelemen, J. M. Fernandez-Abalos, and M. J. Bibb (1999)
Microbiology 145, 2221-2227
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Generalized transduction in Streptomyces coelicolor.
J. Burke, D. Schneider, and J. Westpheling (2001)
PNAS 98, 6289-6294
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