Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 29 April 1988:
Vol. 240. no. 4852, pp. 653 - 656
DOI: 10.1126/science.2452482
Prev | Table of Contents | Next

Articles

Science, Vol 240, Issue 4852, 653-656
Copyright © 1988 by American Association for the Advancement of Science

articles

Highly cooperative opening of calcium channels by inositol 1,4,5-trisphosphate

T Meyer, D Holowka, and L Stryer

Department of Cell Biology, Sherman Fairchild Center, Stanford University School of Medicine, CA 94305.

The kinetics of calcium release by inositol 1,4,5-trisphosphate (IP3) in permeabilized rat basophilic leukemia cells were studied to obtain insight into the molecular mechanism of action of this intracellular messenger of the phosphoinositide cascade. Calcium release from intracellular storage sites was monitored with fura-2, a fluorescent indicator. The dependence of the rate of calcium release on the concentration of added IP3 in the 4 to 40 nM range showed that channel opening requires the binding of at least three molecules of IP3. Channel opening occurred in the absence of added adenosine triphosphate, indicating that IP3 acts directly on the channel or on a protein that gates it. The channels were opened by IP3 in less than 4 seconds. The highly cooperative opening of calcium channels by nanomolar concentrations of IP3 enables cells to detect and amplify very small changes in the concentration of this messenger in response to hormonal, sensory, and growth control stimuli.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Inositol Trisphosphate Receptor Ca2+ Release Channels.
J. K. Foskett, C. White, K.-H. Cheung, and D.-O. D. Mak (2007)
Physiol Rev 87, 593-658
   Abstract »    Full Text »    PDF »
Potentiation of P2Y Receptors by Physiological Elevations of Extracellular K+ via a Mechanism Independent of Ca2+ Influx.
S. J. Pitt, J. Martinez-Pinna, E. A. Barnard, and M. P. Mahaut-Smith (2005)
Mol. Pharmacol. 67, 1705-1713
   Abstract »    Full Text »    PDF »
Transactivation of Sphingosine-1-Phosphate Receptors by Fc{varepsilon}RI Triggering Is Required for Normal Mast Cell Degranulation and Chemotaxis.
P. S. Jolly, M. Bektas, A. Olivera, C. Gonzalez-Espinosa, R. L. Proia, J. Rivera, S. Milstien, and S. Spiegel (2004)
J. Exp. Med. 199, 959-970
   Abstract »    Full Text »    PDF »
Origin and Mechanisms of Ca2+ Waves in Smooth Muscle as Revealed by Localized Photolysis of Caged Inositol 1,4,5-Trisphosphate.
J. G. McCarron, D. MacMillan, K. N. Bradley, S. Chalmers, and T. C. Muir (2004)
J. Biol. Chem. 279, 8417-8427
   Abstract »    Full Text »    PDF »
Three-dimensional Rearrangements within Inositol 1,4,5-Trisphosphate Receptor by Calcium.
K. Hamada, A. Terauchi, and K. Mikoshiba (2003)
J. Biol. Chem. 278, 52881-52889
   Abstract »    Full Text »    PDF »
Spontaneous Channel Activity of the Inositol 1,4,5-Trisphosphate (InsP3) Receptor (InsP3R). Application of Allosteric Modeling to Calcium and InsP3 Regulation of InsP3R Single-channel Gating.
D.-O. D. Mak, S. M.J. McBride, and J. K. Foskett (2003)
J. Gen. Physiol. 122, 583-603
   Abstract »    Full Text »    PDF »
Disruption of lipid order by short-chain ceramides correlates with inhibition of phospholipase D and downstream signaling by Fc{epsilon}RI.
A. Gidwani, H. A. Brown, D. Holowka, and B. Baird (2003)
J. Cell Sci. 116, 3177-3187
   Abstract »    Full Text »    PDF »
Nuclear and cytosolic calcium are regulated independently.
M. F. Leite, E. C. Thrower, W. Echevarria, P. Koulen, K. Hirata, A. M. Bennett, B. E. Ehrlich, and M. H. Nathanson (2003)
PNAS 100, 2975-2980
   Abstract »    Full Text »    PDF »
Control of IP3-mediated Ca2+ puffs in Xenopus laevis oocytes by the Ca2+-binding protein parvalbumin.
L. M John, M. Mosquera-Caro, P. Camacho, and J. D Lechleiter (2001)
J. Physiol. 535, 3-16
   Abstract »    Full Text »    PDF »
Mechanism of receptor-oriented intercellular calcium wave propagation in hepatocytes.
G. DUPONT, T. TORDJMANN, C. CLAIR, S. SWILLENS, M. CLARET, and L. COMBETTES (2000)
FASEB J 14, 279-289
   Abstract »    Full Text »
ATP Regulation of Type 1 Inositol 1,4,5-Trisphosphate Receptor Channel Gating by Allosteric Tuning of Ca2+ Activation.
D.-O. D. Mak, S. McBride, and J. K. Foskett (1999)
J. Biol. Chem. 274, 22231-22237
   Abstract »    Full Text »    PDF »
Norepinephrine-induced Ca2+ waves depend on InsP3 and ryanodine receptor activation in vascular myocytes.
F.-X. Boittin, N. Macrez, G. Halet, and J. Mironneau (1999)
Am J Physiol Cell Physiol 277, C139-C151
   Abstract »    Full Text »    PDF »
Inositol 1,4,5-tris-phosphate activation of inositol tris-phosphate receptor Ca2+ channel by ligand tuning of Ca2+ inhibition.
D.-O. D. Mak, S. McBride, and J. K. Foskett (1998)
PNAS 95, 15821-15825
   Abstract »    Full Text »    PDF »
Physiological Features of Visceral Smooth Muscle Cells, With Special Reference to Receptors and Ion Channels.
H. KURIYAMA, K. KITAMURA, T. ITOH, and R. INOUE (1998)
Physiol Rev 78, 811-920
   Abstract »    Full Text »    PDF »
Inositol 1,4,5-Trisphosphate and Calcium Regulate the Calcium Channel Function of the Hepatic Inositol 1,4,5-Trisphosphate Receptor.
J.-F. Dufour, I. M. Arias, and T. J. Turner (1997)
J. Biol. Chem. 272, 2675-2681
   Abstract »    Full Text »    PDF »
Reversible Desensitization of Inositol Trisphosphate-induced Calcium Release Provides a Mechanism for Repetitive Calcium Spikes.
E. Oancea and T. Meyer (1996)
J. Biol. Chem. 271, 17253-17260
   Abstract »    Full Text »    PDF »
Calcium as a coagonist of inositol 1,4,5-trisphosphate-induced calcium release.
E. Finch, T. Turner, and S. Goldin (1991)
Science 252, 443-446
   Abstract »    PDF »
Localized all-or-none calcium liberation by inositol trisphosphate.
I Parker and I Ivorra (1990)
Science 250, 977-979
   Abstract »    PDF »
Molecular Mechanisms of Phospholipid Signaling Pathways in Mammalian Nerve Cells.
M.R. Hanley, T.R. Jackson, W.T. Cheung, M. Dreher, A. Gatti, P. Hawkins, S.I. Patterson, M. Vallejo, A.P. Dawson, and O. Thastrup (1988)
Cold Spring Harb Symp Quant Biol 53, 435-445
   Abstract »    PDF »
Inositol 1,4,5-Trisphosphate Receptor/Ca2+ Channel Modulatory Role of Chromogranin A, a Ca2+ Storage Protein of Secretory Granules.
S. H. Yoo and C. J. Jeon (2000)
J. Biol. Chem. 275, 15067-15073
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)