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Science 29 April 1988:
Vol. 240. no. 4852, pp. 640 - 642
DOI: 10.1126/science.2834821
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Articles

Science, Vol 240, Issue 4852, 640-642
Copyright © 1988 by American Association for the Advancement of Science

articles

Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro

JA Imlay, SM Chin, and S Linn

Department of Biochemistry, University of California, Berkeley 94720.

Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.


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Hydrogen Peroxide in Lens Care: Unfounded Indictments-Reply.
B. J. Tripathi and R. C. Tripathi (1990)
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DNA damage and oxygen radical toxicity.
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Science 240, 1302-1309
   Abstract »    PDF »
Activation of the Cytoplasmic c-Abl Tyrosine Kinase by Reactive Oxygen Species.
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