Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 29 April 1988:
Vol. 240. no. 4852, pp. 640 - 642
DOI: 10.1126/science.2834821
Prev | Table of Contents | Next

Articles

Science, Vol 240, Issue 4852, 640-642
Copyright © 1988 by American Association for the Advancement of Science

articles

Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro

JA Imlay, SM Chin, and S Linn

Department of Biochemistry, University of California, Berkeley 94720.

Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The iron-sulfur clusters of dehydratases are primary intracellular targets of copper toxicity.
L. Macomber and J. A. Imlay (2009)
PNAS 106, 8344-8349
   Abstract »    Full Text »    PDF »
Molecular properties, functions, and potential applications of NAD kinases.
F. Shi, Y. Li, Y. Li, and X. Wang (2009)
Acta Biochim Biophys Sin 41, 352-361
   Abstract »    Full Text »    PDF »
Roles of Agrobacterium tumefaciens RirA in Iron Regulation, Oxidative Stress Response, and Virulence.
P. Ngok-Ngam, N. Ruangkiattikul, A. Mahavihakanont, S. S. Virgem, R. Sukchawalit, and S. Mongkolsuk (2009)
J. Bacteriol. 191, 2083-2090
   Abstract »    Full Text »    PDF »
Killing niche competitors by remote-control bacteriophage induction.
L. Selva, D. Viana, G. Regev-Yochay, K. Trzcinski, J. M. Corpa, i. Lasa, R. P. Novick, and J. R. Penades (2009)
PNAS 106, 1234-1238
   Abstract »    Full Text »    PDF »
Evaluation of plating media for recovering Salmonella from thermally treated egg albumen.
J. B. Gurtler (2009)
J. Appl. Poult. Res. 18, 297-309
   Abstract »    Full Text »    PDF »
Survival of Nosocomial Bacteria and Spores on Surfaces and Inactivation by Hydrogen Peroxide Vapor.
J. A. Otter and G. L. French (2009)
J. Clin. Microbiol. 47, 205-207
   Abstract »    Full Text »    PDF »
Trade-Off between Iron Uptake and Protection against Oxidative Stress: Deletion of cueO Promotes Uropathogenic Escherichia coli Virulence in a Mouse Model of Urinary Tract Infection.
J. J. Tree, G. C. Ulett, C.-L. Y. Ong, D. J. Trott, A. G. McEwan, and M. A. Schembri (2008)
J. Bacteriol. 190, 6909-6912
   Abstract »    Full Text »    PDF »
De novo CoA biosynthesis is required to maintain DNA integrity during development of the Drosophila nervous system.
F. Bosveld, A. Rana, P. E. van der Wouden, W. Lemstra, M. Ritsema, H. H. Kampinga, and O. C. M. Sibon (2008)
Hum. Mol. Genet. 17, 2058-2069
   Abstract »    Full Text »    PDF »
Activated neutrophils induce an hMSH2-dependent G2/M checkpoint arrest and replication errors at a (CA)13-repeat in colon epithelial cells.
C Campregher, M G Luciani, and C Gasche (2008)
Gut 57, 780-787
   Abstract »    Full Text »    PDF »
Nitric Oxide Evokes an Adaptive Response to Oxidative Stress by Arresting Respiration.
M. Husain, T. J. Bourret, B. D. McCollister, J. Jones-Carson, J. Laughlin, and A. Vazquez-Torres (2008)
J. Biol. Chem. 283, 7682-7689
   Abstract »    Full Text »    PDF »
Broad-spectrum in vitro antibacterial activities of clay minerals against antibiotic-susceptible and antibiotic-resistant bacterial pathogens.
S. E. Haydel, C. M. Remenih, and L. B. Williams (2008)
J. Antimicrob. Chemother. 61, 353-361
   Abstract »    Full Text »    PDF »
The SH3-like Domain Switches Its Interaction Partners to Modulate the Repression Activity of Mycobacterial Iron-dependent Transcription Regulator in Response to Metal Ion Fluctuations.
C. Liu, K. Mao, M. Zhang, Z. Sun, W. Hong, C. Li, B. Peng, and Z. Chang (2008)
J. Biol. Chem. 283, 2439-2453
   Abstract »    Full Text »    PDF »
Agrobacterium tumefaciens fur Has Important Physiological Roles in Iron and Manganese Homeostasis, the Oxidative Stress Response, and Full Virulence.
W. Kitphati, P. Ngok-ngam, S. Suwanmaneerat, R. Sukchawalit, and S. Mongkolsuk (2007)
Appl. Envir. Microbiol. 73, 4760-4768
   Abstract »    Full Text »    PDF »
3,4-Dichloropropionaniline Suppresses Normal Macrophage Function.
I. V. Ustyugova, L. L. Frost, K. VanDyke, K. M. Brundage, R. Schafer, and J. B. Barnett (2007)
Toxicol. Sci. 97, 364-374
   Abstract »    Full Text »    PDF »
Molecular characterization of the Fur protein of Listeria monocytogenes.
N. Ledala, S. L. Pearson, B. J. Wilkinson, and R. K. Jayaswal (2007)
Microbiology 153, 1103-1111
   Abstract »    Full Text »    PDF »
Use of Hydrogen Peroxide Vapor for Deactivation of Mycobacterium tuberculosis in a Biological Safety Cabinet and a Room.
L. Hall, J. A. Otter, J. Chewins, and N. L. Wengenack (2007)
J. Clin. Microbiol. 45, 810-815
   Abstract »    Full Text »    PDF »
Intracellular Copper Does Not Catalyze the Formation of Oxidative DNA Damage in Escherichia coli.
L. Macomber, C. Rensing, and J. A. Imlay (2007)
J. Bacteriol. 189, 1616-1626
   Abstract »    Full Text »    PDF »
Catalase (KatA) and Alkyl Hydroperoxide Reductase (AhpC) Have Compensatory Roles in Peroxide Stress Resistance and Are Required for Survival, Persistence, and Nasal Colonization in Staphylococcus aureus.
K. Cosgrove, G. Coutts, I.-M. Jonsson, A. Tarkowski, J. F. Kokai-Kun, J. J. Mond, and S. J. Foster (2007)
J. Bacteriol. 189, 1025-1035
   Abstract »    Full Text »    PDF »
Implication of (Mn)superoxide dismutase of Enterococcus faecalis in oxidative stress responses and survival inside macrophages..
N. Verneuil, A. Maze, M. Sanguinetti, J.-M. Laplace, A. Benachour, Y. Auffray, J.-C. Giard, and A. Hartke (2006)
Microbiology 152, 2579-2589
   Abstract »    Full Text »    PDF »
Retinal light damage: structural and functional effects of the antioxidant glutathione peroxidase-1..
A. D. Gosbell, N. Stefanovic, L. L. Scurr, J. Pete, I. Kola, I. Favilla, and J. B. de Haan (2006)
Invest. Ophthalmol. Vis. Sci. 47, 2613-2622
   Abstract »    Full Text »    PDF »
Lack of the antioxidant glutathione peroxidase-1 does not increase atherosclerosis in C57BL/J6 mice fed a high-fat diet.
J. B. de Haan, P. K. Witting, N. Stefanovic, J. Pete, M. Daskalakis, I. Kola, R. Stocker, and J. J. Smolich (2006)
J. Lipid Res. 47, 1157-1167
   Abstract »    Full Text »    PDF »
Evidence that feedback inhibition of NAD kinase controls responses to oxidative stress.
J. H. Grose, L. Joss, S. F. Velick, and J. R. Roth (2006)
PNAS 103, 7601-7606
   Abstract »    Full Text »    PDF »
Reactive Oxygen Species-Mediated Mitochondria-to-Nucleus Signaling: A Key to Aging and Radical-Caused Diseases.
P. Storz (2006)
Sci. STKE 2006, re3
   Abstract »    Full Text »    PDF »
Endonuclease IV and Exonuclease III are involved in the repair and mutagenesis of DNA lesions induced by UVB in Escherichia coli.
L.L. Souza, I.R. Eduardo, M. Padula, and A.C. Leitao (2006)
Mutagenesis 21, 125-130
   Abstract »    Full Text »    PDF »
Glutathione depletion by buthionine sulfoximine induces DNA deletions in mice.
R. Reliene and R. H. Schiestl (2006)
Carcinogenesis 27, 240-244
   Abstract »    Full Text »    PDF »
Azurin of Pathogenic Neisseria spp. Is Involved in Defense against Hydrogen Peroxide and Survival within Cervical Epithelial Cells.
H.-J. Wu, K. L. Seib, J. L. Edwards, M. A. Apicella, A. G. McEwan, and M. P. Jennings (2005)
Infect. Immun. 73, 8444-8448
   Abstract »    Full Text »    PDF »
Substantial DNA damage from submicromolar intracellular hydrogen peroxide detected in Hpx- mutants of Escherichia coli.
S. Park, X. You, and J. A. Imlay (2005)
PNAS 102, 9317-9322
   Abstract »    Full Text »    PDF »
Comparative Analysis of Differentially Expressed Genes in Shewanella oneidensis MR-1 following Exposure to UVC, UVB, and UVA Radiation.
X. Qiu, G. W. Sundin, L. Wu, J. Zhou, and J. M. Tiedje (2005)
J. Bacteriol. 187, 3556-3564
   Abstract »    Full Text »    PDF »
Polyphosphate Kinase Protects Salmonella enterica from Weak Organic Acid Stress.
M. Price-Carter, T. G. Fazzio, E. I. Vallbona, and J. R. Roth (2005)
J. Bacteriol. 187, 3088-3099
   Abstract »    Full Text »    PDF »
Regulation of NAD Synthesis by the Trifunctional NadR Protein of Salmonella enterica.
J. H. Grose, U. Bergthorsson, and J. R. Roth (2005)
J. Bacteriol. 187, 2774-2782
   Abstract »    Full Text »    PDF »
Second-Generation Recombination-Based In Vivo Expression Technology for Large-Scale Screening for Vibrio cholerae Genes Induced during Infection of the Mouse Small Intestine.
C. G. Osorio, J. A. Crawford, J. Michalski, H. Martinez-Wilson, J. B. Kaper, and A. Camilli (2005)
Infect. Immun. 73, 972-980
   Abstract »    Full Text »    PDF »
Preferential binding and structural distortion by Fe2+ at RGGG-containing DNA sequences correlates with enhanced oxidative cleavage at such sequences.
P. Rai, D. E. Wemmer, and S. Linn (2005)
Nucleic Acids Res. 33, 497-510
   Abstract »    Full Text »    PDF »
8-Oxo-7,8-Dihydroguanine Is Removed by a Nucleotide Excision Repair-Like Mechanism in Porphyromonas gingivalis W83.
N. A. Johnson, R. McKenzie, L. McLean, L. C. Sowers, and H. M. Fletcher (2004)
J. Bacteriol. 186, 7697-7703
   Abstract »    Full Text »    PDF »
Characterization of a dam mutant of Haemophilus influenzae Rd.
P. Zaleski and A. Piekarowicz (2004)
Microbiology 150, 3773-3781
   Abstract »    Full Text »    PDF »
Fusidic Acid-Resistant Mutants of Salmonella enterica Serovar Typhimurium Have Low Levels of Heme and a Reduced Rate of Respiration and Are Sensitive to Oxidative Stress.
M. Macvanin, A. Ballagi, and D. Hughes (2004)
Antimicrob. Agents Chemother. 48, 3877-3883
   Abstract »    Full Text »    PDF »
Effect of N-Acetyl Cysteine on Oxidative DNA Damage and the Frequency of DNA Deletions in Atm-Deficient Mice.
R. Reliene, E. Fischer, and R. H. Schiestl (2004)
Cancer Res. 64, 5148-5153
   Abstract »    Full Text »    PDF »
Cells have distinct mechanisms to maintain protection against different reactive oxygen species: Oxidative-stress-response genes.
G. W. Thorpe, C. S. Fong, N. Alic, V. J. Higgins, and I. W. Dawes (2004)
PNAS 101, 6564-6569
   Abstract »    Full Text »    PDF »
Hydrogen Peroxide Induces Topoisomerase I-mediated DNA Damage and Cell Death.
P. Daroui, S. D. Desai, T.-K. Li, A. A. Liu, and L. F. Liu (2004)
J. Biol. Chem. 279, 14587-14594
   Abstract »    Full Text »    PDF »
Gene expression profiling in respiratory tissues from rats exposed to mainstream cigarette smoke.
S. Gebel, B. Gerstmayer, A. Bosio, H.-J. Haussmann, E. V. Miert, and T. Muller (2004)
Carcinogenesis 25, 169-178
   Abstract »    Full Text »    PDF »
Factors Contributing to Hydrogen Peroxide Resistance in Streptococcus pneumoniae Include Pyruvate Oxidase (SpxB) and Avoidance of the Toxic Effects of the Fenton Reaction.
C. D. Pericone, S. Park, J. A. Imlay, and J. N. Weiser (2003)
J. Bacteriol. 185, 6815-6825
   Abstract »    Full Text »    PDF »
Similarities and differences in the responses of microorganisms to biocides.
A. D. Russell (2003)
J. Antimicrob. Chemother. 52, 750-763
   Abstract »    Full Text »    PDF »
Effects of Hydrogen Peroxide upon Nicotinamide Nucleotide Metabolism in Escherichia coli: CHANGES IN ENZYME LEVELS AND NICOTINAMIDE NUCLEOTIDE POOLS AND STUDIES OF THE OXIDATION OF NAD(P)H BY Fe(III).
J. L. Brumaghim, Y. Li, E. Henle, and S. Linn (2003)
J. Biol. Chem. 278, 42495-42504
   Abstract »    Full Text »    PDF »
Inhibition of bacterial DNA replication by zinc mobilization during nitrosative stress.
J. M. Schapiro, S. J. Libby, and F. C. Fang (2003)
PNAS 100, 8496-8501
   Abstract »    Full Text »    PDF »
Alkyl Hydroperoxide Reductase Dependent on Thioredoxin-Like Protein from Pyrococcus horikoshii.
Y. Kashima and K. Ishikawa (2003)
J. Biochem. 134, 25-29
   Abstract »    Full Text »    PDF »
The Molecular Evolution of Catalatic Hydroperoxidases: Evidence for Multiple Lateral Transfer of Genes Between Prokaryota and from Bacteria into Eukaryota.
M. G. Klotz and P. C. Loewen (2003)
Mol. Biol. Evol. 20, 1098-1112
   Abstract »    Full Text »    PDF »
High Levels of Intracellular Cysteine Promote Oxidative DNA Damage by Driving the Fenton Reaction.
S. Park and J. A. Imlay (2003)
J. Bacteriol. 185, 1942-1950
   Abstract »    Full Text »    PDF »
Micromolar concentrations of hydrogen peroxide induce oxidative DNA lesions more efficiently than millimolar concentrations in mammalian cells.
J. Nakamura, E. R. Purvis, and J. A. Swenberg (2003)
Nucleic Acids Res. 31, 1790-1795
   Abstract »    Full Text »    PDF »
Polyamines protect Escherichia coli cells from the toxic effect of oxygen.
M. K. Chattopadhyay, C. W. Tabor, and H. Tabor (2003)
PNAS 100, 2261-2265
   Abstract »    Full Text »    PDF »
The Escherichia coli Methyl-Directed Mismatch Repair System Repairs Base Pairs Containing Oxidative Lesions.
J. Wyrzykowski and M. R. Volkert (2003)
J. Bacteriol. 185, 1701-1704
   Abstract »    Full Text »    PDF »
Molecular Basis of H2O2 Resistance Mediated by Streptococcal Dpr. DEMONSTRATION OF THE FUNCTIONAL INVOLVEMENT OF THE PUTATIVE FERROXIDASE CENTER BY SITE-DIRECTED MUTAGENESIS IN STREPTOCOCCUS SUIS.
A. T. Pulliainen, S. Haataja, S. Kahkonen, and J. Finne (2003)
J. Biol. Chem. 278, 7996-8005
   Abstract »    Full Text »    PDF »
The Iron-Binding Protein Dps Confers Hydrogen Peroxide Stress Resistance to Campylobacter jejuni.
T. Ishikawa, Y. Mizunoe, S.-i. Kawabata, A. Takade, M. Harada, S. N. Wai, and S.-i. Yoshida (2003)
J. Bacteriol. 185, 1010-1017
   Abstract »    Full Text »    PDF »
Global genome removal of thymine glycol in Escherichia coli requires endonuclease III but the persistence of processed repair intermediates rather than thymine glycol correlates with cellular sensitivity to high doses of hydrogen peroxide.
M. Alanazi, S. A. Leadon, and I. Mellon (2002)
Nucleic Acids Res. 30, 4583-4591
   Abstract »    Full Text »    PDF »
Gene Expression after Treatment with Hydrogen Peroxide, Menadione, or t-Butyl Hydroperoxide in Breast Cancer Cells.
Y.-Y. E. Chuang, Y. Chen, Gadisetti, V. R. Chandramouli, J. A. Cook, D. Coffin, M.-H. Tsai, W. DeGraff, H. Yan, S. Zhao, et al. (2002)
Cancer Res. 62, 6246-6254
   Abstract »    Full Text »    PDF »
Iron Supplementation Increases Disease Activity and Vitamin E Ameliorates the Effect in Rats with Dextran Sulfate Sodium-Induced Colitis.
J. Carrier, E. Aghdassi, J. Cullen, and J. P. Allard (2002)
J. Nutr. 132, 3146-3150
   Abstract »    Full Text »    PDF »
Reduced Flavins Promote Oxidative DNA Damage in Non-respiring Escherichia coli by Delivering Electrons to Intracellular Free Iron.
A. N. Woodmansee and J. A. Imlay (2002)
J. Biol. Chem. 277, 34055-34066
   Abstract »    Full Text »    PDF »
A Salmonella enterica Serovar Typhimurium hemA Mutant Is Highly Susceptible to Oxidative DNA Damage.
M. Elgrably-Weiss, S. Park, E. Schlosser-Silverman, I. Rosenshine, J. Imlay, and S. Altuvia (2002)
J. Bacteriol. 184, 3774-3784
   Abstract »    Full Text »    PDF »
ideR, an Essential Gene in Mycobacterium tuberculosis: Role of IdeR in Iron-Dependent Gene Expression, Iron Metabolism, and Oxidative Stress Response.
G. M. Rodriguez, M. I. Voskuil, B. Gold, G. K. Schoolnik, and I. Smith (2002)
Infect. Immun. 70, 3371-3381
   Abstract »    Full Text »    PDF »
Mechanisms of 2-Butoxyethanol Carcinogenicity: Studies on Syrian Hamster Embryo (SHE) Cell Transformation.
J. Park, L. M. Kamendulis, and J. E. Klaunig (2002)
Toxicol. Sci. 68, 43-50
   Abstract »    Full Text »    PDF »
Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization.
A. Schutzendubel and A. Polle (2002)
J. Exp. Bot. 53, 1351-1365
   Abstract »    Full Text »    PDF »
Kinetics of gene expression profiling in Swiss 3T3 cells exposed to aqueous extracts of cigarette smoke.
A. Bosio, C. Knorr, U. Janssen, S. Gebel, H.-J. Haussmann, and T. Muller (2002)
Carcinogenesis 23, 741-748
   Abstract »    Full Text »    PDF »
Heat-induced formation of reactive oxygen species and 8-oxoguanine, a biomarker of damage to DNA.
V. I. Bruskov, L. V. Malakhova, Z. K. Masalimov, and A. V. Chernikov (2002)
Nucleic Acids Res. 30, 1354-1363
   Abstract »    Full Text »    PDF »
Crossing Over Between Regions of Limited Homology in Escherichia coli: RecA-Dependent and RecA-Independent Pathways.
S. T. Lovett, R. L. Hurley, V. A. Sutera Jr., R. H. Aubuchon, and M. A. Lebedeva (2002)
Genetics 160, 851-859
   Abstract »    Full Text »    PDF »
Overexpression of Copper Zinc Superoxide Dismutase Suppresses Human Glioma Cell Growth.
Y. Zhang, W. Zhao, H. J. Zhang, F. E. Domann, and L. W. Oberley (2002)
Cancer Res. 62, 1205-1212
   Abstract »    Full Text »    PDF »
DNA damage and cell cycle checkpoints in hyperoxic lung injury: braking to facilitate repair.
M. A. O'Reilly (2001)
Am J Physiol Lung Cell Mol Physiol 281, L291-L305
   Abstract »    Full Text »    PDF »
Viability of rep recA Mutants Depends on Their Capacity To Cope with Spontaneous Oxidative Damage and on the DnaK Chaperone Protein.
M.-F. Bredèche, S. D. Ehrlich, and B. Michel (2001)
J. Bacteriol. 183, 2165-2171
   Abstract »    Full Text »
Plasma Vitamin C Levels Are Decreased and Correlated With Brain Damage in Patients With Intracranial Hemorrhage or Head Trauma.
M. C. Polidori, P. Mecocci, and B. Frei (2001)
Stroke 32, 898-902
   Abstract »    Full Text »    PDF »
Vascular release of nonheme iron in perfused rabbit lungs.
Y.-C. T. Huang, A. J. Ghio, E. Nozik-Grayck, and C. A. Piantadosi (2001)
Am J Physiol Lung Cell Mol Physiol 280, L474-L481
   Abstract »    Full Text »    PDF »
Increased Cytochrome c-Mediated DNA Fragmentation and Cell Death in Manganese-Superoxide Dismutase-Deficient Mice After Exposure to Subarachnoid Hemolysate.
P. G. Matz, M. Fujimura, A. Lewen, Y. Morita-Fujimura, and P. H. Chan (2001)
Stroke 32, 506-515
   Abstract »    Full Text »    PDF »
In Staphylococcus aureus, Fur Is an Interactive Regulator with PerR, Contributes to Virulence, and Is Necessary for Oxidative Stress Resistance through Positive Regulation of Catalase and Iron Homeostasis.
M. J. Horsburgh, E. Ingham, and S. J. Foster (2001)
J. Bacteriol. 183, 468-475
   Abstract »    Full Text »
The Activity of NF-{{kappa}}B in Swiss 3T3 Cells Exposed to Aqueous Extracts of Cigarette Smoke Is Dependent on Thioredoxin.
S. Gebel and T. Muller (2001)
Toxicol. Sci. 59, 75-81
   Abstract »    Full Text »    PDF »
Iron Overload and Heart Fibrosis in Mice Deficient for Both {beta}2-Microglobulin and Rag1.
M. M. Santos, M. de Sousa, L. H. P. M. Rademakers, H. Clevers, J. J. M. Marx, and M. W. Schilham (2000)
Am. J. Pathol. 157, 1883-1892
   Abstract »    Full Text »    PDF »
Cell Death After Exposure to Subarachnoid Hemolysate Correlates Inversely With Expression of CuZn-Superoxide Dismutase Editorial Comment.
P. G. Matz, J.-C. Copin, P. H. Chan, and R. L. Macdonald (2000)
Stroke 31, 2450-2459
   Abstract »    Full Text »    PDF »
Repair of DNA Lesions Induced by Hydrogen Peroxide in the Presence of Iron Chelators in Escherichia coli: Participation of Endonuclease IV and Fpg.
R. S. Galhardo, C. E. B. Almeida, A. C. Leitão, and J. B. Cabral-Neto (2000)
J. Bacteriol. 182, 1964-1968
   Abstract »    Full Text »
Role of Iron and Superoxide for Generation of Hydroxyl Radical, Oxidative DNA Lesions, and Mutagenesis in Escherichia coli.
T. Nunoshiba, F. Obata, A. C. Boss, S. Oikawa, T. Mori, S. Kawanishi, and K. Yamamoto (1999)
J. Biol. Chem. 274, 34832-34837
   Abstract »    Full Text »    PDF »
Yeast Mitochondrial Protein, Nfs1p, Coordinately Regulates Iron-Sulfur Cluster Proteins, Cellular Iron Uptake, and Iron Distribution.
J. Li, M. Kogan, S. A. B. Knight, D. Pain, and A. Dancis (1999)
J. Biol. Chem. 274, 33025-33034
   Abstract »    Full Text »    PDF »
OxyR and SoxRS Regulation of fur.
M. Zheng, B. Doan, T. D. Schneider, and G. Storz (1999)
J. Bacteriol. 181, 4639-4643
   Abstract »    Full Text »
Role of Superoxide Dismutase Activity in the Physiology of Porphyromonas gingivalis.
M. C. Lynch and H. K. Kuramitsu (1999)
Infect. Immun. 67, 3367-3375
   Abstract »    Full Text »    PDF »
Activation of topoisomerase II-mediated excision of chromosomal DNA loops during oxidative stress.
T.-K. Li, A. Y. Chen, C. Yu, Y. Mao, H. Wang, and L. F. Liu (1999)
Genes & Dev. 13, 1553-1560
   Abstract »    Full Text »
Sequence-specific DNA Cleavage by Fe2+-mediated Fenton Reactions Has Possible Biological Implications.
E. S. Henle, Z. Han, N. Tang, P. Rai, Y. Luo, and S. Linn (1999)
J. Biol. Chem. 274, 962-971
   Abstract »    Full Text »    PDF »
Characterization of a Peroxide-Resistant Mutant of the Anaerobic Bacterium Bacteroides fragilis.
E. R. Rocha and C. J. Smith (1998)
J. Bacteriol. 180, 5906-5912
   Abstract »    Full Text »
Induction of microsatellite instability by oxidative DNA damage.
A. L. Jackson, R. Chen, and L. A. Loeb (1998)
PNAS 95, 12468-12473
   Abstract »    Full Text »    PDF »
Mapping of the DNA Binding Domain of the Copper-responsive Transcription Factor Mac1 from Saccharomyces cerevisiae.
L. T. Jensen, M. C. Posewitz, C. Srinivasan, and D. R. Winge (1998)
J. Biol. Chem. 273, 23805-23811
   Abstract »    Full Text »    PDF »
Nitric oxide enhances hydrogen peroxide-mediated endothelial permeability in vitro.
N. Okayama, C. G. Kevil, L. Correia, D. Jourd'Heuil, M. Itoh, M. B. Grisham, and J. S. Alexander (1997)
Am J Physiol Cell Physiol 273, C1581-C1587
   Abstract »    Full Text »    PDF »
Inactivation of Dehydratase [4Fe-4S] Clusters and Disruption of Iron Homeostasis upon Cell Exposure to Peroxynitrite.
K. Keyer and J. A. Imlay (1997)
J. Biol. Chem. 272, 27652-27659
   Abstract »    Full Text »    PDF »
Infrared spectral models demonstrate that exposure to environmental chemicals leads to new forms of DNA.
D. C. Malins, N. L. Polissar, and S. J. Gunselman (1997)
PNAS 94, 3611-3615
   Abstract »    Full Text »    PDF »
Models of DNA structure achieve almost perfect discrimination between normal prostate, benign prostatic hyperplasia (BPH), and adenocarcinoma and have a high potential for predicting BPH and prostate cancer.
D. C. Malins, N. L. Polissar, and S. J. Gunselman (1997)
PNAS 94, 259-264
   Abstract »    Full Text »    PDF »
Induction of p21 Mediated by Reactive Oxygen Species Formed during the Metabolism of Aziridinylbenzoquinones by HCT116 Cells.
X. Qiu, H. J. Forman, A. H. Schonthal, and E. Cadenas (1996)
J. Biol. Chem. 271, 31915-31921
   Abstract »    Full Text »    PDF »
Superoxide accelerates DNA damage by elevating free-iron levels.
K. Keyer and J. A. Imlay (1996)
PNAS 93, 13635-13640
   Abstract »    Full Text »    PDF »
Oxidative Damage to DNA Constituents by Iron-mediated Fenton Reactions. THE DEOXYCYTIDINE FAMILY.
Y. Luo, E. S. Henle, and S. Linn (1996)
J. Biol. Chem. 271, 21167-21176
   Abstract »    Full Text »    PDF »
Subarachnoid Injections of Lysed Blood Induce the hsp70 Stress Gene and Produce DNA Fragmentation in Focal Areas of the Rat Brain.
P. Matz, P. Weinstein, B. States, J. Honkaniemi, and F. R. Sharp (1996)
Stroke 27, 504-513
   Abstract »    Full Text »
Hydrogen Peroxide in Lens Care: Unfounded Indictments-Reply.
B. J. Tripathi and R. C. Tripathi (1990)
Arch Ophthalmol 108, 913-914
   Abstract »    PDF »
Hydrogen Peroxide Damage to Human Corneal Epithelial Cells In Vitro: Implications for Contact Lens Disinfection Systems.
B. J. Tripathi and R. C. Tripathi (1989)
Arch Ophthalmol 107, 1516-1519
   Abstract »    PDF »
DNA damage and oxygen radical toxicity.
J. Imlay and S Linn (1988)
Science 240, 1302-1309
   Abstract »    PDF »
Activation of the Cytoplasmic c-Abl Tyrosine Kinase by Reactive Oxygen Species.
X. Sun, P. Majumder, H. Shioya, F. Wu, S. Kumar, R. Weichselbaum, S. Kharbanda, and D. Kufe (2000)
J. Biol. Chem. 275, 17237-17240
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)