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Science 4 March 1988:
Vol. 239. no. 4844, pp. 1121 - 1128
DOI: 10.1126/science.2830671
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Articles

Science, Vol 239, Issue 4844, 1121-1128
Copyright © 1988 by American Association for the Advancement of Science

articles

Insertional mutagenesis of the Drosophila genome with single P elements

L Cooley, R Kelley, and A Spradling

Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210.

A versatile genetic method for identifying and cloning Drosophila melanogaster genes affecting any recognizable phenotype is described. Strains are constructed in which the insertion of a single P transposable element has caused a new mutation, greatly simplifying the genetic and molecular analysis of the affected gene. Mutagenesis is initiated by crossing two strains, each of which contains a specially designed P element. One element (jumpstarter), encoding P element transposase, efficiently mobilizes the second nonautonomous transposon (mutator), whose structure facilitates selection and cloning of new insertion mutations. Random mutator transpositions are captured in individual stocks that no longer contain jumpstarter, where they remain stable. This method was used to construct 1300 single P element insertion stocks which were then screened for recessive mutations. A library of single-element insertion strains will allow the structure and function of Drosophila genes to be readily correlated, and should have many other applications in Drosophila molecular genetics.


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Development 126, 5353-5364
   Abstract »    PDF »
Cyclin A and B functions in the early Drosophila embryo.
L. Stiffler, J. Ji, S Trautmann, C Trusty, and G Schubiger (1999)
Development 126, 5505-5513
   Abstract »    PDF »
The Drosophila dead ringer gene is required for early embryonic patterning through regulation of argos and buttonhead expression.
T Shandala, R. Kortschak, S Gregory, and R Saint (1999)
Development 126, 4341-4349
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Extended Life-Span and Stress Resistance in the Drosophila Mutant methuselah.
Y. Lin, L. Seroude, and S. Benzer (1998)
Science 282, 943-946
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Y-Linked Male Sterile Mutations Induced by P Element in Drosophila melanogaster.
P. Zhang and R. L. Stankiewicz (1998)
Genetics 150, 735-744
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Kinesin Light Chains Are Essential for Axonal Transport in Drosophila.
J. G. Gindhart Jr., C. J. Desai, S. Beushausen, K. Zinn, and L. S.B. Goldstein (1998)
J. Cell Biol. 141, 443-454
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Heterosis for Viability, Fecundity, and Male Fertility in Drosophila melanogaster : Comparison of Mutational and Standing Variation.
J. D. Fry, S. L. Heinsohn, and T. F. C. Mackay (1998)
Genetics 148, 1171-1188
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DRhoGEF2 encodes a member of the Dbl family of oncogenes and controls cell shape changes during gastrulation in Drosophila.
U. Häcker and N. Perrimon (1998)
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A Chromatin-associated Kinesin-related Protein Required for Normal Mitotic Chromosome Segregation in Drosophila.
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J. Cell Biol. 139, 1361-1371
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Identification of genes required for Drosophila eye development using a phenotypic enhancer-trap.
F. Pignoni, B. Hu, and S. L. Zipursky (1997)
PNAS 94, 9220-9225
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A. Paululat, A. Goubeaud, C. Damm, S. Knirr, S. Burchard, and R. Renkawitz-Pohl (1997)
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KLP38B: A Mitotic Kinesin-related Protein That Binds PP1.
L. Alphey, L. Parker, G. Hawcroft, Y. Guo, K. Kaiser, and G. Morgan (1997)
J. Cell Biol. 138, 395-409
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Insertional Mutation of the Drosophila Nuclear Lamin Dm0 Gene Results in Defective Nuclear Envelopes, Clustering of Nuclear Pore Complexes, and Accumulation of Annulate Lamellae.
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J. Cell Biol. 137, 1001-1016
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P element insertion-dependent gene activation in the Drosophila eye.
B. A. Hay, R. Maile, and G. M. Rubin (1997)
PNAS 94, 5195-5200
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The k43 gene, required for chorion gene amplification and diploid cell chromosome replication, encodes the Drosophila homolog of yeast origin recognition complex subunit 2.
G. Landis, R. Kelley, A. C. Spradling, and J. Tower (1997)
PNAS 94, 3888-3892
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Prevention of insect-borne disease: An approach using transgenic symbiotic bacteria.
R. V. Durvasula, A. Gumbs, A. Panackal, O. Kruglov, S. Aksoy, R. B. Merrifield, F. F. Richards, and C. B. Beard (1997)
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U Hacker, X Lin, and N Perrimon (1997)
Development 124, 3565-3573
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The Drosophila G-protein-coupled receptor kinase homologue Gprk2 is required for egg morphogenesis.
L. Schneider and A. Spradling (1997)
Development 124, 2591-2602
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A novel group of pumilio mutations affects the asymmetric division of germline stem cells in the Drosophila ovary.
H Lin and A. Spradling (1997)
Development 124, 2463-2476
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Characterization of Drosophila Tyramine beta -Hydroxylase Gene and Isolation of Mutant Flies Lacking Octopamine.
M. Monastirioti, C. E. Linn, Jr., and K. White (1996)
J. Neurosci. 16, 3900-3911
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A maternal requirement for glutamine synthetase I for the mitotic cycles of syncytial Drosophila embryos.
L. Frenz and D. Glover (1996)
J. Cell Sci. 109, 2649-2660
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The Drosophila genes grauzone and cortex are necessary for proper female meiosis.
A. Page and T. Orr-Weaver (1996)
J. Cell Sci. 109, 1707-1715
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canoe encodes a novel protein containing a GLGF/DHR motif and functions with Notch and scabrous in common developmental pathways in Drosophila..
H Miyamoto, I Nihonmatsu, S Kondo, R Ueda, S Togashi, K Hirata, Y Ikegami, and D Yamamoto (1995)
Genes & Dev. 9, 612-625
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A role for the Drosophila bag-of-marbles protein in the differentiation of cystoblasts from germline stem cells.
D McKearin and B Ohlstein (1995)
Development 121, 2937-2947
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The Drosophila E74 gene is required for metamorphosis and plays a role in the polytene chromosome puffing response to ecdysone.
J. Fletcher, K. Burtis, D. Hogness, and C. Thummel (1995)
Development 121, 1455-1465
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The mutant not enough muscles (nem) reveals reduction of the Drosophila embryonic muscle pattern.
S Burchard, A Paululat, U Hinz, and R Renkawitz-Pohl (1995)
J. Cell Sci. 108, 1443-1454
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Loss of circadian behavioral rhythms and per RNA oscillations in the Drosophila mutant timeless.
A Sehgal, J. Price, B Man, and M. Young (1994)
Science 263, 1603-1606
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orb is required for anteroposterior and dorsoventral patterning during Drosophila oogenesis..
L B Christerson and D M McKearin (1994)
Genes & Dev. 8, 614-628
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A central role for epidermal segment border cells in the induction of muscle patterning in the Drosophila embryo.
T Volk and K VijayRaghavan (1994)
Development 120, 59-70
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Initial organization of the Drosophila dorsoventral axis depends on an RNA-binding protein encoded by the squid gene..
R L Kelley (1993)
Genes & Dev. 7, 948-960
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Mutation of twins encoding a regulator of protein phosphatase 2A leads to pattern duplication in Drosophila imaginal discs..
T Uemura, K Shiomi, S Togashi, and M Takeichi (1993)
Genes & Dev. 7, 429-440
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Mutations affecting the cytoskeletal organization of syncytial Drosophila embryos.
W Sullivan, P Fogarty, and W Theurkauf (1993)
Development 118, 1245-1254
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Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.
A. Brand and N Perrimon (1993)
Development 118, 401-415
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Gene expression during imaginal disc regeneration detected using enhancer-sensitive P-elements.
W. Brook, L. Ostafichuk, J Piorecky, M. Wilkinson, D. Hodgetts, and M. Russell (1993)
Development 117, 1287-1297
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The Drosophila Ras2 and Rop gene pair: a dual homology with a yeast Ras-like gene and a suppressor of its loss-of-function phenotype.
A Salzberg, N Cohen, N Halachmi, Z Kimchie, and Z Lev (1993)
Development 117, 1309-1319
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hu-li tai shao, a gene required for ring canal formation during Drosophila oogenesis, encodes a homolog of adducin..
L Yue and A C Spradling (1992)
Genes & Dev. 6, 2443-2454
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Of fin and fur: mutational analysis of vertebrate embryonic development..
J Rossant and N Hopkins (1992)
Genes & Dev. 6, 1-13
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Toward cloning and mapping the genome of Drosophila.
J Merriam, M Ashburner, D. Hartl, and F. Kafatos (1991)
Science 254, 221-225
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Targeted gene replacement in Drosophila via P element-induced gap repair.
G. Gloor, N. Nassif, D. Johnson-Schlitz, C. Preston, and W. Engels (1991)
Science 253, 1110-1117
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Functional dissection of the paired segmentation gene in Drosophila embryos..
D Morrissey, D Askew, L Raj, and M Weir (1991)
Genes & Dev. 5, 1684-1696
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Exploring the Role of Drosophila Cyclin A in the Regulation of S Phase.
C.F. Lehner, N. Yakubovich, and P.H. O'Farrell (1991)
Cold Spring Harb Symp Quant Biol 56, 465-475
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bag-of-marbles: a Drosophila gene required to initiate both male and female gametogenesis..
D M McKearin and A C Spradling (1990)
Genes & Dev. 4, 2242-2251
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Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector..
E Bier, H Vaessin, S Shepherd, K Lee, K McCall, S Barbel, L Ackerman, R Carretto, T Uemura, and E Grell (1989)
Genes & Dev. 3, 1273-1287
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P-element-mediated enhancer detection: a versatile method to study development in Drosophila..
H J Bellen, C J O'Kane, C Wilson, U Grossniklaus, R K Pearson, and W J Gehring (1989)
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