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Science 26 February 1988:
Vol. 239. no. 4843, pp. 1024 - 1026
DOI: 10.1126/science.2449730
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Articles

Science, Vol 239, Issue 4843, 1024-1026
Copyright © 1988 by American Association for the Advancement of Science

articles

Voltage-sensitive calcium channels in normal and transformed 3T3 fibroblasts

CF Chen, MJ Corbley, TM Roberts, and P Hess

Department of Physiology, Harvard Medical School, Boston, MA 02115.

Patch clamp recordings of whole-cell and single channel currents revealed the presence of two voltage-sensitive calcium channel types in the membrane of 3T3 fibroblasts. The two calcium channel types were identified by their unitary properties and pharmacological sensitivities. Both calcium channel types were present in all control 3T3 cells, but one type was selectively suppressed in 3T3 cells that had been transformed by activated c-H-ras, EJ-ras, v-fms, or polyoma middle T oncogenes. The presence of voltage-sensitive calcium channels in these nonexcitable cells and the control of their functional expression by transforming oncogenes raises questions about their role in the control of calcium-sensitive processes such as cell motility, cytoskeletal organization, and cell growth.


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