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Science 19 February 1988:
Vol. 239. no. 4842, pp. 914 - 916
DOI: 10.1126/science.2829358
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Articles

Science, Vol 239, Issue 4842, 914-916
Copyright © 1988 by American Association for the Advancement of Science

articles

Autocrine stimulation of intracellular PDGF receptors in v-sis-transformed cells

MT Keating and LT Williams

Howard Hughes Medical Institute, San Francisco, CA.

Autocrine activation of platelet-derived growth factor (PDGF) receptors is the mechanism of transformation by the v-sis oncogene. Since the addition of PDGF does not transform normal cells, autocrine mechanisms may involve unique pathways of receptor activation. In this study autocrine stimulation of the PDGF receptor was observed in v-sis-transformed normal rat kidney (NRK) cells. In contrast to receptor activation in normal cells, autocrine activation of PDGF receptors in v-sis-transformed cells occurred in intracellular compartments, disrupting receptor processing and diverting receptors and their precursors to a chloroquine-sensitive degradation pathway. These findings show that intracellular activation of receptors by autocrine mechanisms may play a role in cell transformation.


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